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Cortex lycii active site and preparation method and application thereof

A technology of the active part and Digupi, which is applied in the field of preparation of the active part of traditional Chinese medicine, can solve the problem of insufficient stability of curative effect, and achieve the effect of clear mechanism of action, definite curative effect and low cost

Inactive Publication Date: 2014-04-02
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Digupi is often used in the treatment of diabetes in single herbs or compound prescriptions. There are many studies on the mechanism of pharmacological action, but most of them use the crude extract as the research object and treatment material, so the curative effect is not stable and very optimistic. However, if its extract The refining of the main active ingredients in the drug will be of great benefit to the determination of its curative effect and preliminary qualitative and quantitative methods

Method used

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  • Cortex lycii active site and preparation method and application thereof
  • Cortex lycii active site and preparation method and application thereof
  • Cortex lycii active site and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. The preparation method of the active site of Cortex Digustifolia

[0029] S1. After crushing Digupi, use 10 times the amount of water to heat and reflux at 100°C for 2 extractions, each time for 120 minutes, then concentrate the extract to 1 / 4 of its original volume by suction filtration, and add 95% ethanol for alcohol extraction. The final ethanol concentration is about 75%, and the supernatant is concentrated under pressure to obtain a stock solution.

[0030] S2. Pass the stock solution through the macroporous adsorption resin, the ratio (weight) of Digupi original medicinal material to resin is 1:3, wash the resin column with deionized water 3 times the volume of the resin bed, collect the eluate, and concentrate under reduced pressure to obtain Sample 1.

[0031] S3. Exchange sample 1 with a strong-acid cation-exchange resin (cross-linked polystyrene-based strong acid resin with a model number of 001×7), rinse the resin column with deionized water 3 ...

Embodiment 2

[0033] Example 2. A liquid phase analysis method for the active part of Digustifolia

[0034] FMOC-Cl (fluorenylmethoxycarbonyl chloride) is a relatively new amino derivatization reagent, which is mainly suitable for the derivatization of primary and secondary amines, and the derivatives after the reaction have UV absorption. The anti-diabetic active molecule of Digupi has a secondary amine structure, so FMOC-Cl can be used as a derivatization reagent for derivatization. Take 50 μl of sample 3 solution in Example 1 in a 1.5ml centrifuge tube, add 200 μl of 0.4mol / L potassium borate buffer solution, then add 250 μl of 5 mmol / L FMOC-Cl (50% acetonitrile configuration), shake well, and place at room temperature After 30 minutes, add 25 μl of 1mol / L glycine (Gly) to stop the reaction, then add 80 μl of acetic acid solution with a volume fraction of 1%, add water to make up to 1000 μl, and finally filter the solution with an organic membrane to obtain the test solution. HP...

Embodiment 3

[0035] Example 3 Inhibition of α-glucosidase activity by the active site of Cortex Dictica

[0036] Inhibition of α-glucosidase activity by the active part of Digustifolia (prepared in Example 1): Take 1.85 ml of pH6.8 phosphate buffer solution, add 0.1 U·ml -1 50 μL of α-glucosidase, different concentrations of Digustifolia active site solution (0, 1, 2, 3, 4, 5, 6, 7 mg·ml -1 ) 50 μL, 0.0116 mol L -1 pNPG 50 μL, after incubating the above reaction system in a constant temperature water bath at 37 °C for 30 min, immediately add 0.1 mol L -1 Na 2 CO 3 8 ml of the solution was used to stop the reaction, and three replicates were performed for each concentration, and the average value was taken.

[0037] According to the above-mentioned method and calculation formula, obtain the inhibitory action figure of Digupi active site to α-glucosidase, such as figure 2 shown. It can be clearly seen from the figure that the active part of Digupi has a strong inhibitory effect...

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Abstract

The invention relates to the field of traditional Chinese medicine active site preparation, and discloses a cortex lycii active site, a preparation method and an application thereof. The preparation method comprises the following steps: S1, crushing cortex lycii, extracting with water, concentrating the extract, adding ethanol for alcohol precipitation, concentrating the supernatant to obtain a stock solution; S2, allowing the stock solution to pass through macroporous adsorption resin, collecting the eluate, drying by distillation, and concentrating to obtain a sample 1; S3, adsorbing the sample 1 by strong acid cation exchange resin, washing the resin column with water, then eluting with ammonia water, collecting the ammonia water eluate, concentrating to obtain a sample 2; S4, adsorbing the sample 2 by strong base anion exchange resin, collecting water eluate, concentrating and drying to obtain the cortex lycii active site. The cortex lycii active site prepared by the invention has a high content of alkaloid active components, and is applicable to prevention and treatment of diseases of diabetes, obesity, hyperlipidemia, and the like.

Description

technical field [0001] The invention relates to the field of preparation of active parts of traditional Chinese medicines, and more specifically relates to an active part of Digustifolia and its preparation method and application. Background technique [0002] The fourth edition of Diabetes Map data released in October 2009 showed that there were as many as 43.2 million diabetics in China, but the latest epidemiological survey results published in the "New England Journal" on March 24, 2010 showed that China has replaced India has become the country with the most diabetic patients in the world. The latest study reflects the rapid increase in diabetes prevalence in China in recent years. Based on this, IDF predicts that the number of people with diabetes in the world will approach 500 million by 2030. Drugs for treating diabetes at home and abroad include oral hypoglycemic agents, insulin, exogenous GLP-1 receptor agonists, GLP-1 analogs, etc. Although western medicines hav...

Claims

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Application Information

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IPC IPC(8): A61K36/815A61P3/10A61P3/04A61P3/06
Inventor 周光雄焦呈亮
Owner JINAN UNIVERSITY
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