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Streptomyces griseus CCCQ171 and application thereof

A technology of Streptomyces griseus and strains, applied in the application, bacteria, fungicides and other directions, can solve the problems of aggravating the pollution of pesticide environment, the difference in drug resistance of root black rot pathogen strains, and increasing the cost of disease prevention and control.

Active Publication Date: 2014-04-02
GUANGZHOU ZHENGE AGRI SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extensive and long-term continuous use of chemical pesticides in tobacco production and the unreasonable use of technology have led to the resistance of tobacco root black rot pathogens, and there are differences in the resistance of root black rot pathogen strains from different sources
The emergence of drug-resistant strains not only increases the cost of disease control, but also aggravates the pollution of the environment and the destruction of the ecosystem by the use of pesticides, and also directly affects the quality of tobacco leaves

Method used

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  • Streptomyces griseus CCCQ171 and application thereof
  • Streptomyces griseus CCCQ171 and application thereof
  • Streptomyces griseus CCCQ171 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Screening and identification of embodiment 1CCCQ171 bacterial strain

[0020] Seventeen strains of actinomycetes with antibacterial activity were obtained after primary screening and secondary screening of multiple strains isolated from tobacco field soil in Chongqing. The first screening adopts the plate confrontation method, and the second screening adopts the chloroform fumigation method. Finally, through the disease control and control effect test, it is determined that the Streptomyces griseochromogenes CCCQ171 strain of the present invention has a better control effect on tobacco root black rot.

[0021] 1.1 Observation of colony characteristics

[0022] Yeast refined maltose agar medium (ISP-2), oat flour agar medium (ISP-3), inorganic salt starch agar medium (ISP-4), glycerol asparagine agar medium (ISP-5) and potato Soaking juice medium, Czapck's medium and modified Gao's No. 1 medium were used to make solid plate medium. Streak inoculation of the CCCQ171 st...

Embodiment 2

[0036] Embodiment 2CCCQ171 strain bacteriostatic spectrum test

[0037] Using the plate confrontation method, take different bacterial strains for activation, use a sterilized puncher (diameter 6mm) to punch holes in the edge area of ​​the colony to make bacterial slices, transfer them to the center of the PDA plate, and culture them at 28°C for 48 hours. After colonization , inoculate the CCCQ171 strain on both sides at a distance of 30 mm from the center, and each treatment was repeated 3 times. Incubate at a constant temperature of 28°C for 7 days, measure the bacteriostatic bandwidth, measure the colony diameter of the pathogen, and calculate the bacteriostatic rate. The antagonistic effects of the CCCQ171 strain on tobacco pathogens are shown in Table 1.

[0038] Table 1 CCCQ171 strain antimicrobial spectrum screening

[0039]

[0040] Note: "-" in Table 1 means no inhibition zone.

[0041] Determination of the antagonistic activity of CCCQ171 strain against 9 kinds o...

Embodiment 3

[0042] Application of embodiment 3 CCCQ171 bacterial strain

[0043] 3.1 Inhibitory effect of fermentation broth of CCCQ171 strain on spore germination

[0044] Tobacco root black rot fungus was cultured on potato dextrose agar medium for 10 days, and the spores were scraped, and the spore concentration was adjusted to about 1×10 by adding sterile water. 5 pc / mL, spare.

[0045] Prepare the modified Gaoshi No. 1 medium according to the following formula, and then autoclave it for later use: KNO 3 1.0g; K 2 HPO 4 0.5g; MgSO 4 · gS 2 O0.5g; NaCl0.5g; FeSO 4 · eS 2 O0.01g; soluble starch 20.0g; agar 17.0g; pH: 7.2~7.4. CCCQ171 strain was cultured on the modified Gaoshi No. 1 slant medium at 28°C for 7-10 days at a constant temperature. After it produced a sufficient amount of spores, pour sterile water along the slant, just submerged the slant after slanting, and scrape it with a sterile ring. Take surface bacteria to make bacteria suspension. Put 100mL of modified Gaos...

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Abstract

The invention discloses a streptomyces griseus (streptomyces griseochromogenes) CCCQ171 and an application thereof. The preservation number of the streptomyces griseus CCCQ171 is CGMCC No.6227, and the streptomyces griseus is named a Jialing bacterium according to the producing area. The invention also discloses an application of the streptomyces griseus CCCQ171 in an agro-microbial agent and the agro-microbial agent thereof. The agro-microbial agent contains the streptomyces griseus CCCQ171, and is used for preventing and treating tobacco diseases or inducing the tobacco to generate resistance on the tobacco diseases. The streptomyces griseus CCCQ171 is separated from soil of a tobacco field, and has natural harmonious compatibility with natural ecology. Compared with a chemical pesticide, the agro-microbial agent is free of toxic and side effects on the soil ecology, and free of a residue. Therefore, the streptomyces griseus has potential business development and application value in a biological control practice of the diseases.

Description

technical field [0001] The invention relates to the field of tobacco disease prevention and control of microorganisms and their application, in particular to a strain of Streptomyces griseochromogenes and its application. Background technique [0002] In the long-term evolution process of nature, there is a common phenomenon of interaction between plants and surrounding environmental microorganisms. There are two types of beneficial plant-microbe symbiosis: those with a nutritional function, such as nitrogen-fixing bacteria or mycorrhizal fungi, and those with a defensive function, such as endophytes and epiphytes that inhibit pathogens. Both classes of beneficial symbiotic microorganisms can enhance plant fitness. Agrochemicals (fertilizers and pesticides) and "intensive farming" cultivation methods play an important role in agricultural production. Under this cultivation method, the plant-microbe balance system in cultivated crops is broken, and there is a lack of benefi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/545
Inventor 王泊理马冠华温好菊赵勇
Owner GUANGZHOU ZHENGE AGRI SCI & TECH CO LTD
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