Holothurian culture water body modifier capable of relieving environmental stress reaction on holothurians, and preparation method and application thereof

A technology for stress response and aquaculture water, which is applied in the field of aquaculture and marine microorganisms. It can solve the problems of excessive chemical residues and antibiotic residues, pollution of aquaculture water, high mortality of sea cucumbers, etc., and achieve good suspension and controllability of sinking and floating. Avoid acidification and odor, reduce the effect of stress response

Inactive Publication Date: 2014-04-02
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the current situation in the prior art, such as serious pollution of aquaculture water quality, frequent occurrence of diseases, high mortality rate of sea cucumbers, excessive heavy metals, chemical residues...

Method used

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  • Holothurian culture water body modifier capable of relieving environmental stress reaction on holothurians, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of probiotic stock solution

[0037] Add Bacillus subtilis YB-1CGMCCNo.5116, Bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, which grow well on LB agar slant medium, respectively to 2116E medium, pH7.0~7.5 , shake culture at 37°C for 12-16 hours, and then mix it at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×10 8 cfu / ml.

[0038] 2. Liquid fermentation culture

[0039] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0040] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0041] 3. Spray drying treatment

[0042] The composite ads...

Embodiment 2

[0045] 1. Preparation of probiotic stock solution

[0046] Add Bacillus subtilis YB-1CGMCCNo.5116, Bacillus cereus YB-2CGMCCNo.5117 and Bacillus flexus XH-1CGMCCNo.5115, which grow well on LB agar slant medium, respectively to 2116E medium, pH7.0~7.5 , shake culture at 37°C for 12-16 hours, and then mix them at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0047] 2. Liquid fermentation culture

[0048] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0049] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0050] 3. Spray drying treatment

[0051] The adsorption car...

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Abstract

The invention relates to a holothurian culture water body modifier capable of relieving environmental stress reaction on holothurians, and a preparation method and application thereof. The preparation method comprises the following steps: through a marine microorganism technology, based on physiological habits and culture characteristics of holothurians, separating and screening Bacillus flexus CGMCC No.5115, Bacillus subtilis CGMCC No.5116 and Bacillus cereus CGMCC No.5117 from a holothurian culture environment; selecting corn flour and bean pulp flour as main culture raw materials, and fermenting at 28-37 DEG C for 24-48h; and selecting zeolite, meerschaum and rice hull as adsorption and suspension compound carriers, and processing to prepare the holothurian culture water body modifier capable of relieving environmental stress reaction on holothurians. The product has high tolerance to the water body environment and has favorable floatability, suspensibility and floating/sinking controllability, thereby being beneficial to three-dimensional stereoscopical purifying effect of a marine culture water body; and harmful substances such as Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+>, Cu<2+>, other heavy metal ions and the like in the water body can be adsorbed, thus greatly reducing the content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, preventing occurrence of holothurian disease and increasing the culture output and quality of holothurians.

Description

technical field [0001] The invention relates to the technical field of aquaculture and marine microbes, and in particular relates to a sea cucumber culture water improver capable of alleviating the stress response of the environment to sea cucumbers, as well as its preparation method and application. Background technique [0002] Seawater is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects the growth status of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people's demand for sea cucumbers is increasing day by day. Under the circumstances that natural fishing cannot meet the market demand, the sea cucumber farming industry has transformed from the traditional extensive management and resource-dependent farming methods to the current intensive and factory farming methods...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/02C02F3/34C12R1/125C12R1/085C12R1/07
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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