Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for immobilizing urokinase by employing magnetic tragacanth microspheres

A technology of gum tragacanth and urokinase, which is applied in biomedical engineering and biological fields, can solve the problems of poor biocompatibility of magnetic microspheres, unsatisfactory magnetic response, and complicated operation, and achieves uniform particle size and strong magnetic response. , good dispersion effect

Inactive Publication Date: 2014-04-09
INNER MONGOLIA UNIV OF SCI & TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some studies on the use of magnetic polymer microspheres to immobilize enzymes and proteins, such as the use of magnetic agarose microspheres, gelatin microspheres, polyacrylamide microspheres, polysaccharide microspheres, etc. to immobilize proteins and enzymes, but some magnetic microspheres The biocompatibility of the ball is poor, the magnetic response of some is not ideal, and some are expensive and cumbersome to operate
Using tragacanth gum to coat magnetic particles for the immobilization of urokinase is easy to operate, cheap, and has good magnetic responsiveness, dispersibility and biocompatibility. Related technical research has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for immobilizing urokinase by employing magnetic tragacanth microspheres
  • Method for immobilizing urokinase by employing magnetic tragacanth microspheres
  • Method for immobilizing urokinase by employing magnetic tragacanth microspheres

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] ① Preparation of magnetic tragacanth particles

[0019] Dissolve 0.5g of tragacanth in 40ml of re-distilled water, transfer it into a four-necked flask equipped with a stirrer, a condenser and a nitrogen inlet, and add 60mL of 0.15mol / L ferrous chloride solution and 0.010% ferric chloride solution dropwise successively. 10 mL of hydrogen oxide solution, stirred (400 r / min), 40 mL of 3 mol / L potassium hydroxide solution was added dropwise, and at the same time under nitrogen protection, the reaction was carried out in a boiling water bath for 4.5 h.

[0020] ②Fixation of urokinase

[0021] Weigh 0.2g of magnetic tragacanth particles, add 18mg of urokinase, shake at 4°C for 6h, fully wash with re-distilled water, and separate the mixture in a magnetic field with a strength of 100Gs until no enzyme protein is washed out, freeze-vacuum-dry to obtain magnetic Immobilized urokinase.

Embodiment 2

[0023] ① Preparation of magnetic tragacanth particles

[0024] Dissolve 1.2g of tragacanth in 40ml of redistilled water, transfer it into a four-necked flask equipped with a stirrer, a condenser and a nitrogen inlet, and add 0.20mol / L ferrous chloride solution 70mL and 0.012% ferric chloride solution dropwise successively. Hydrogen oxide solution 10mL, stirred (360r / min), dropwise added 45ml of 3.5mol / L sodium hydroxide solution, while nitrogen protection, reacted in a boiling water bath for 3h.

[0025] ②Fixation of urokinase

[0026] Weigh 0.1 g of magnetic tragacanth particles, treated with 0.2% glutaraldehyde for 1 h, and then cross-linked with 7 mg of urokinase in boric acid buffer (pH 8.8), shaken at room temperature for 8 h, and washed with distilled water. Separate in a magnetic field of 120Gs until enzyme-free protein washes out.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for immobilizing urokinase by employing magnetic tragacanth microspheres, and belongs to the technical fields of biomedical engineering and biology. The method comprises the following steps: and forming ultrafine particles from ferrous oxide ions as an oxidant in a polymerization manner by using an emulsifying composite technology under an alkaline condition, and then coating the surfaces of the magnetic ultrafine particles with the urokinase, so as to prepare the Fe3O4-urokinase composite microspheres. The microspheres are uniform in particle size, strong in magnetic responsiveness, and good in dispersity in water. Thus, the urokinase is immobilized by adopting the microspheres as a carrier through a physical adsorption method and a crosslinking method. The magnetic immobilized urokinase has magnetic responsiveness, so that the magnetic immobilized urokinase is guided to move towards a targeted part inside a body and be located and centralized by using an in vitro magnetic field, targeted drug delivery of the urokinase inside the body is achieved, the drug efficiency is improved, and the urokinase has the characteristics of high efficiency and low toxicity.

Description

technical field [0001] The invention relates to a method for immobilizing urokinase by using magnetic tragacanth gum microspheres, belonging to the fields of biomedical engineering and biotechnology. Background technique [0002] Thrombotic disease is a disease with high incidence, death and disability rate worldwide. Although there are many antithrombotic drugs, there are still many shortcomings in the aspects of drug targeting, efficacy and side effects. Therefore, the development of antithrombotic drugs with low price, high efficiency, strong targeting, long in vivo half-life and small side effects has become the direction of domestic and foreign research and development of drugs for the treatment of thrombosis diseases. Urokinase is an antithrombotic drug widely used in clinical practice, but if the dose is too small, the ideal thrombolytic effect cannot be achieved. The thrombolytic effect is a very meaningful issue. [0003] Magnetic-polymer microspheres are a kind ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10
Inventor 巩东辉
Owner INNER MONGOLIA UNIV OF SCI & TECH