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Extraction method of lincomycin

A technology of lincomycin and extraction method, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low purity, large solvent consumption, low yield of lincomycin, etc. The effect of low impurity content, improved extraction efficiency and high product yield

Active Publication Date: 2014-04-16
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the problems of low lincomycin yield, low purity and large solvent consumption in the prior art, provide a kind of solvent loss, easy to operate, and the product lincomycin purity can reach more than 98.5%. Lincomycin Extraction Method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 150 kg of lincomycin fermentation broth, add oxalic acid to adjust the pH to 3, and then filter through the first ceramic ultrafiltration membrane, the ceramic membrane has a pore size of 0.05 μm, a pressure of 0.2 MPa, and a membrane surface flow rate of 2 m / s. Take the filtered acidification solution and use concentrated alkali to adjust the pH to 11, then pass the alkalization solution through the second ceramic ultrafiltration membrane again, the ceramic membrane has a pore size of 0.05 μm, a pressure of 0.1 MPa, and a membrane surface flow rate of 2 m / s. Add 2-octanol to the alkalization solution, the dosage is 1 / 10 of the volume of the second ceramic membrane clear solution, stir at room temperature for 5 minutes and let it stand for stratification. The stratification process is faster, the interface is clear, and there is no emulsification phenomenon. Secondary octanol was added to the waste liquid after the first extraction, the dosage was 1 / 10 of the volume...

Embodiment 2

[0037] Regarding the selection of the extractant, in the present embodiment, some common extractants are used in the extraction step. The difference from Example 1 is that the extractants shown in Table 1 are used respectively. The extraction method is the same, and it is obtained The final product testing results are shown in Table 1.

[0038] Table 1 Effect of different extractants on product results

[0039] Extracting agent 2-octyl alcohol Butanol Isobutanol Isoamyl alcohol Second-pentanol Content % 97.9 95.1 96.8 95.7 96.1 Impurity B % 0.43 0.75 0.87 0.75 0.71 Yield % 95.2 92.5 90.2 91.3 88.4

[0040] As can be seen from the table, with secondary octanol as the extractant, the content, yield and other indicators of the product obtained are better than other extractants.

[0041]

Embodiment 3

[0043] Take 150kg of lincomycin fermentation broth, add oxalic acid to adjust the pH to 4, and then filter through the first ceramic ultrafiltration membrane. The ceramic membrane has a pore size of 0.02 μm, a pressure of 0.1 MPa, and a membrane surface flow velocity of 5 m / s. Take the filtered acidification solution and use concentrated alkali to adjust the pH to 11, then pass the alkalization solution through the second ceramic ultrafiltration membrane again. Add 2-octanol to the alkaline solution, the dosage is 1 / 8 of the volume of the second ceramic membrane clear solution, stir at room temperature for 5 minutes and let it stand for stratification. The stratification process is fast, the interface is clear, and no emulsification occurs. Add secondary octanol to the waste liquid after the first extraction, the dosage is 1 / 8 of the volume of the second ceramic membrane liquid, stir at room temperature for 5 minutes and let stand to separate. Combine the organic phases and ad...

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Abstract

The invention provides an extraction method of lincomycin. The extraction method mainly comprises the following steps: adjusting acidity of fermentation liquor, filtering by using a ceramic ultrafiltration membrane, then adjusting alkalinity, then filtering by using a ceramic ultrafiltration membrane again, then carrying out primary sec-octyl alcohol extraction and hydrochloric acid back-extraction, adjusting alkalinity of the primary back extraction liquor and then filtering by using a ceramic ultrafiltration membrane again; carrying out secondary sec-octyl alcohol extraction and hydrochloric acid back-extraction and adding acetone to the secondary back extraction liquor to carry out evaporative crystallization. According to the extraction method provided by the invention, the yield of the lincomycin is high, the color grade of the lincomycin can be improved, and the B component content in the lincomycin is reduced. According to the extraction method of the lincomycin, the feed liquid quality of other extraction technologies can be improved by a membrane treatment process, the integral extraction efficiency is further improved, the residual volume of sec-octyl in raffinate in the extraction procedure is reduced, the product quality is improved, and meanwhile, the COD (Chemical Oxygen Demand) in produced wastewater is reduced. The extraction method has certain economic benefit and environmental benefit.

Description

[0001] technical field [0002] The invention relates to a method for extracting lincomycin, in particular to a method for extracting lincomycin by combining membrane filtration and extraction. [0003] Background technique [0004] Lincomycin, the English name is Lincomycin. It acts on the 50S subunit of the ribosome of sensitive bacteria to prevent the extension of the pus chain, thereby inhibiting the protein synthesis of bacterial cells. Common name: lincomycin, Chinese aliases: lincomycin, lincomycin, lincomycin; chemical name: 6-(1-methyl-trans-4-propyl-L-2-pyrrolidine carboxamide )-1-thio-6,8-dideoxy-D-erythro-α-D-galactopyranoside hydrochloride monohydrate. Molecular formula: C 18 h 34 N 2 o 6 S·HCl·H 2 O, molecular weight: 461.02, this product is white crystalline powder; slightly or special odor; bitter taste. This product is easily soluble in water or methanol, slightly soluble in ethanol. [0005] At present, the production process of lincomycin is rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/16C07H1/08
Inventor 秦泗光熊福军陈磊王贝贝彭文博张宏
Owner JIANGSU JIUWU HITECH
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