Method and device of separating and extracting amino acid products from protein hydrolysates

A protein and amino acid technology, applied in chemical instruments and methods, preparation of organic compounds, preparation of sulfides, etc., can solve the problems of no separation and extraction, and achieve the effect of expanding separation, good separation effect and high yield

Active Publication Date: 2014-05-14
广州博采生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the amino acid molecular composition of any protein, aromatic amino acid substances (phenylalanine, tyrosine, tryptophan) occupy a considerable proportion, but in this patent, there is no separation and extraction of aromatic amino acid substances

Method used

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  • Method and device of separating and extracting amino acid products from protein hydrolysates
  • Method and device of separating and extracting amino acid products from protein hydrolysates
  • Method and device of separating and extracting amino acid products from protein hydrolysates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Put 100 grams of pig blood powder protein into an 800mL round bottom flask, add 300mL of 6mol / L concentrated hydrochloric acid, heat to above 110°C, hydrolyze for 20 hours, collect the protein hydrolyzate, dilute it with water, let it flow through a 10cm, 15cm high, type I activated carbon adsorption chromatographic column with granular activated carbon body inside, then wash the chromatographic column with clean water, and use 0.1mol / L hydrochloric acid to prepare a mixed solution of ethanol hydrochloric acid with 50% ethanol concentration to flow through the column After the chromatographic column, wash the chromatographic column with clear water, combine the washing liquid after washing with the mixed solution of ethanol and hydrochloric acid to obtain the collected liquid, and recover the ethanol by distillation to obtain 150mL protein concentrate and 128mL ethanol with a volume percentage of less than 30%. solution, then use 2mol / L ammonia solution to adjust the pH ...

Embodiment 2

[0036]Put 100 grams of duck feathers in an 800mL round bottom flask, add 200mL of 10mol / L concentrated hydrochloric acid, heat to above 110°C, hydrolyze for 7 hours to collect the protein hydrolyzate, dilute it with water, let it flow through a 10cm diameter, 15cm high, type I activated carbon adsorption chromatographic column with granular activated carbon body inside, then wash the chromatographic column with clean water, and use 0.1mol / L hydrochloric acid to prepare a mixed solution of ethanol hydrochloric acid with 50% ethanol concentration to flow through the chromatographic layer After the column, wash the chromatographic column with water, combine the washing liquid and the washing liquid after washing with the mixed solution of ethanol hydrochloric acid to obtain the collected liquid, and recover the ethanol by distillation to obtain a 150mL protein concentrate, and then adjust the protein with 2mol / L ammonia solution. The pH value of the concentrated solution reaches 5...

Embodiment 3

[0039] Put 100 grams of unpeeled rapeseed cake in an 800mL round bottom flask, add 300mL of water to soak for 6 hours, and grind it into a paste, collect the rapeseed cake slurry, adjust the pH value to 7.0, and add 500 units / mL of bacteria Protease was hydrolyzed at 40°C for 3 hours, heated to 100°C and kept for 0.5 hours to inactivate the enzyme, adjusted the pH to 5.0, added 400 units / mL mycoprotease, hydrolyzed at 32°C for 3 hours, raised to 100°C and maintained for 0.5 hours to inactivate the enzyme; Then add 50 units / mL papain and hydrolyze at 31°C for 3 hours, raise the temperature to 100°C and keep it for 0.5 hours to inactivate the enzyme; then add 400 units / mL mycoprotease and hydrolyze at 32°C for 3 hours, filter with Buchner funnel to completely inactivate the enzyme , to obtain the amino acid mixed solution generated by enzymatic hydrolysis; adjust the pH value to 2.5 with 0.1mol / L hydrochloric acid, let it flow through a type I activated carbon adsorption chromato...

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Abstract

The invention discloses a method and device of separating and extracting amino acid products from protein hydrolysates. The method comprises the following steps that amino acid mixed solutions are attenuated by water, flow through an I-shaped column, and are washed, ethanol hydrochloric acid mixed solutions flow through the chromatographic column, collecting fluids are obtained by the combination of washing liquor and the ethanol hydrochloric acid mixed solutions, the collecting fluid is adjusted at the PH of about 5.0 to about 6.0, tyrosine can be acquired through filtration, a filter liquor is neutralized by alkali at the PH of more than 7.0, the filter liquor flows through a III-shaped column, and phenylalanine, tyrosine and/or tryptophan are acquired through separation; the ethanol hydrochloric acid mixed solutions flowed through the I-shaped column is adjusted at the PH of more than 7.0, the ethanol hydrochloric acid mixed solutions flow through a II-shaped column, and light components separation solutions, pulp components separation solutions and propanal components separation solutions can be acquired by the sequential adoption of washing, hydrochloric acid extrication and collecting the scrubbing solution in segments; A subsequent separation is conducted for the separation solutions and all the amino acid products are acquired. The method can increase variety of species and yield rate of a single amino acid, the water body for separation and extraction can be recycled, and waste water meets the requirements of environmental emissions.

Description

Technical field [0001] The present invention involves a method and equipment that separates amino acid products from protein hydrolysis. Background technique [0002] At present, the method of preparing amino acid products is mainly the hydrolyzed protein method and microbial fermentation method. These two methods of preparing amino acid products are carried out in a large amount of water body. Therefore, if only partial separation of amino acid products is extracted from the protein hydrolyzed solution, it will be brought withThe wastewater with amino acid products will seriously pollute the surrounding environment during the discharge process and cannot meet the requirements of environmental protection emissions. [0003] In addition, because various amino acid products are similar and even equal to the nature of the physiotherapy, they will interfere with each other in the process of separation and extraction, which hinders the process of separating all amino acid products and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C323/58C07C319/28C07C319/20C07C319/22C07C279/14C07C277/08C07D233/64C07C229/08C07C227/40C07C227/18C07D207/16C07C229/26C07C229/24C07C229/22C07C229/36C12P13/22C12P13/06C12P13/12C12P13/24C12P13/10C12P13/14C12P13/20C12P13/08C12P13/04
Inventor 邹学满邹凌邹远飞
Owner 广州博采生物科技有限公司
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