Agrobacterium with free living nitrogen fixation, phosphate dissolution and potassium dissolution capacity and application of agrobacterium

A technology of Agrobacterium and ability, applied in the field of agricultural microorganisms, can solve problems such as short validity period, low nitrogen fixation amount, unstable application effect, etc.

Inactive Publication Date: 2014-05-14
NORTHWEST UNIV
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the nitrogen-fixing bacteria strains used in production in my country have low nitrogen-fixing capacity and unstable application effects, and most of the wild nitrogen-fixing bacteria isolated from nature have s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Agrobacterium with free living nitrogen fixation, phosphate dissolution and potassium dissolution capacity and application of agrobacterium
  • Agrobacterium with free living nitrogen fixation, phosphate dissolution and potassium dissolution capacity and application of agrobacterium
  • Agrobacterium with free living nitrogen fixation, phosphate dissolution and potassium dissolution capacity and application of agrobacterium

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] The nitrogen-fixing strain of the present invention is obtained by an ultraviolet-plasma compound mutation breeding method, and the specific steps are as follows:

[0049] 1) Take the Agrobacterium SGD06 screened from the plant rhizosphere in the laboratory as the starting strain;

[0050] 2) Mutagenesis and breeding

[0051] (1) Prepare a single cell suspension of the starting strain SGD06

[0052] The starting strain SGD06 was inoculated in liquid medium A, cultured at 28-32°C, 120-150 rpm for 15-20 hours, centrifuged, washed with sterile saline, placed in a triangular flask containing glass beads, and shaken to make Bacteria suspension dispersed into single cells;

[0053] The composition of the medium A is: 5-10g yeast powder, 2-5g NaCl, 5-10g peptone, FeSO 4 ·7H 2 O 0.005-0.01g, Na 2 MoO 4 ·2H 2 O 0.0025-0.005 g, distilled water 1000ml, pH 7.0~7.2.

[0054] (2) UV mutagenesis

[0055] Adjust the concentration of the bacterial suspension obtained in step (1...

Example Embodiment

[0077] Example 2 SGD06-8-33 Determination of indole acetic acid (IAA) production capacity

[0078] Add 200 μg·ml to a 250 ml Erlenmeyer flask containing 100 ml liquid medium A -1 Of sterile L-tryptophan, inoculate SGD06-8-33 in 50ml liquid medium A, culture at 28℃, 150rpm for 20 hours, take 100 μl (dilute the bacterial solution with medium A to make OD 600 0.7) The bacterial solution was inoculated in the above liquid medium A containing L-tryptophan, cultured at 28°C, 150 rpm for 72 hours, 4000 g, centrifuged for 10 min, and the concentration of IAA in the supernatant was determined by spectrophotometry. The experimental results show that the concentration of IAA produced by SGD06-8-33 is 43.72μg / ml.

Example Embodiment

[0079] Example 3 SGD06-8-33 Determination of Iron Carrier Ability

[0080] Divide the liquid medium A into 250ml Erlenmeyer flasks according to 50ml per bottle, and sterilize them for later use. SGD06-8-33 was inoculated into 50ml liquid medium A, cultured at 28℃, 150rpm for 20 hours, and then 100 μl (dilute the bacterial solution with medium A to make OD 600 0.7) The bacteria solution was inoculated into the above liquid medium A, cultured at 28°C, 150 rpm for 48 hours, the bacteria solution cultured on the above shaker was taken, centrifuged at 3500 rpm for 15 minutes, the supernatant was taken, and the upper was quantitatively determined by the relative absorbance method. The content of siderophores in the clear liquid, A / A r=0.406, indicates that SGD06-8-33 has a strong ability to produce siderophores.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an agrobacterium with free living nitrogen fixation, phosphate dissolution and potassium dissolution capacity. The agrobacterium is classified and named as agrobacterium ((i)Agrobacterium(/i)(i)rsp.(/i))X.L.Zhu.SGD06-8-33 and collected in the China General Microbiological Culture Collection Center, and the collection number is CGMCC No.5457. The strain disclosed by the invention is high in nitrogenase activity, has high phosphate dissolution and potassium dissolution capacity, can generate a certain amount of indoleacetic acid and siderophores without ACC desaminase activity. If crops, such as corns, rapes and leaf mustard, are inoculated by using liquid or a solid microbial inoculum which contains the strain, the growth of the crops can be obviously promoted, and the output of the crops can be increased. The method is simple and convenient in process, rich in raw material source and suitable for industrialized production.

Description

technical field [0001] The invention relates to an Agrobacterium strain capable of autogenous nitrogen fixation, phosphorus and potassium dissolution, and application thereof. The strain is obtained by ultraviolet-plasma compound mutagenesis, can promote the growth of crops, and belongs to the technical field of agricultural microorganisms. technical background [0002] Nitrogen is one of the macroelements necessary for crop growth and development, and contributes 40%-50% to the final yield of crops. Crops take away a large amount of nitrogen from the soil every year. In order to supplement the loss of soil nitrogen, industrial chemical nitrogen fertilizers are still an important source of human nitrogen supply to the soil. Although chemical fertilizers have played a huge role in promoting crop growth and increasing food production, long-term excessive use of chemical fertilizers will cause a series of problems, such as acid deposition, soil compaction, water eutrophication,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N15/01C05G3/00C12R1/01
Inventor 朱晓丽朱燕云董立平
Owner NORTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap