Method for producing 3-hydroxypropionic acid by taking glycerin as raw material fermentation-biological catalysis coupling synthesis system
A hydroxypropionic acid, biocatalysis technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as unrealized industrial production, achieve simple conversion liquid components, easy separation and purification, energy saving and The effect of production costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] (1) After Klebsiella pneumoniae is activated, transfer it to the seed medium (yeast extract 5g / L, tryptone 10g / L, NaCl 10g / L) for cultivation, at 37°C, 180r / min, and cultivate for 6-7 hours to obtain seed culture liquid for use.
[0022] (2) After activating Acetobacter sp., move it to the seed medium (glucose 20g / L, yeast extract 5g / L, peptone 2g / L, pH 6.0) and culture it at 30℃, 220rpm for 16h, then add 10% The amount of inoculum was added to the fermentation medium (glucose 20g / L, yeast extract 5g / L, peptone 2g / L, pH6.0), cultured at 30°C and 220rpm for 48h, centrifuged to obtain the bacteria, and the bacteria were washed with pH6. After washing twice with phosphate buffer solution of pH 6.0, the bacterium was suspended with phosphate buffer solution of pH 6.0 to prepare a bacterium suspension with a concentration of 5 g / L of bacterium for subsequent use.
[0023](3) Using the reactor device to couple the enzyme reaction substrate sustainable g...
Embodiment 2
[0025] The acquisition of Klebsiella pneumoniae seed liquid and Acetobacter sp. bacterial suspension is the same as in Example 1, but the coupling conditions are different. Klebsiella pneumoniae seed liquid was inoculated and fermented with 5% (V / V) inoculum, and the culture conditions were: 5L fermenter, liquid volume 2.5L, 35°C, initial glycerol concentration 18g / L, rotation speed 150r / min, ventilation volume 0.5 L / min, add glycerol from 8h to keep it at about 18g / L, until 22h, use 10mol / L of KOH to control the pH and keep it at 7.8; meanwhile, mix the Klebsiella pneumoniae fermentation broth with the collected 5g / L (dry heavy) Acetobacter sp. bacterial suspension, the reaction conditions are: 32°C, pH6.0, after 48 hours of reaction, the output of 3-hydroxypropionic acid is 45-50g / L.
Embodiment 3
[0027] The acquisition of Klebsiella pneumoniae seed liquid and Acetobacter sp. bacterial suspension is the same as in Example 1, but the coupling conditions are different. Klebsiella pneumoniae seed solution was inoculated and fermented with 5% (V / V) inoculation amount, the culture conditions were: 5L fermenter, liquid volume 2.5L, 35°C, initial glycerol concentration 21g / L, rotation speed 150r / min, ventilation volume 0.5 L / min, add glycerol from 8h to keep it at about 21g / L, until 20h, use 10mol / L of KOH to control the pH and keep it at 8.0; meanwhile, mix the Klebsiella pneumoniae fermentation broth with the collected 5g / L (dry Heavy) Acetobacter sp. bacterial suspension mixed, the reaction conditions are: 35 ℃, pH5.5, after 48 hours of reaction, the output of 3-hydroxypropionic acid is 43-48g / L.
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap