Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing 3-hydroxypropionic acid by taking glycerin as raw material fermentation-biological catalysis coupling synthesis system

A hydroxypropionic acid, biocatalysis technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as unrealized industrial production, achieve simple conversion liquid components, easy separation and purification, energy saving and The effect of production costs

Active Publication Date: 2014-05-14
JIANGNAN UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The synthesis method of 3-hydroxypropionic acid is mainly divided into chemical method and microbial method, but neither of these two methods has realized industrial production at present. Although the production process has been improving, the chemical method has certain shortcomings.
However, since propionic acid and acrylic acid have certain inhibitory effects on bacterial growth, it is particularly important to find new substrates and their transformation strains.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) After Klebsiella pneumoniae is activated, transfer it to the seed medium (yeast extract 5g / L, tryptone 10g / L, NaCl 10g / L) for cultivation, at 37°C, 180r / min, and cultivate for 6-7 hours to obtain seed culture liquid for use.

[0022] (2) After activating Acetobacter sp., move it to the seed medium (glucose 20g / L, yeast extract 5g / L, peptone 2g / L, pH 6.0) and culture it at 30℃, 220rpm for 16h, then add 10% The amount of inoculum was added to the fermentation medium (glucose 20g / L, yeast extract 5g / L, peptone 2g / L, pH6.0), cultured at 30°C and 220rpm for 48h, centrifuged to obtain the bacteria, and the bacteria were washed with pH6. After washing twice with phosphate buffer solution of pH 6.0, the bacterium was suspended with phosphate buffer solution of pH 6.0 to prepare a bacterium suspension with a concentration of 5 g / L of bacterium for subsequent use.

[0023](3) Using the reactor device to couple the enzyme reaction substrate sustainable generation system with ...

Embodiment 2

[0025] The acquisition of Klebsiella pneumoniae seed liquid and Acetobacter sp. bacterial suspension is the same as in Example 1, but the coupling conditions are different. Klebsiella pneumoniae seed liquid was inoculated and fermented with 5% (V / V) inoculum, and the culture conditions were: 5L fermenter, liquid volume 2.5L, 35°C, initial glycerol concentration 18g / L, rotation speed 150r / min, ventilation volume 0.5 L / min, add glycerol from 8h to keep it at about 18g / L, until 22h, use 10mol / L of KOH to control the pH and keep it at 7.8; meanwhile, mix the Klebsiella pneumoniae fermentation broth with the collected 5g / L (dry heavy) Acetobacter sp. bacterial suspension, the reaction conditions are: 32°C, pH6.0, after 48 hours of reaction, the output of 3-hydroxypropionic acid is 45-50g / L.

Embodiment 3

[0027] The acquisition of Klebsiella pneumoniae seed liquid and Acetobacter sp. bacterial suspension is the same as in Example 1, but the coupling conditions are different. Klebsiella pneumoniae seed solution was inoculated and fermented with 5% (V / V) inoculation amount, the culture conditions were: 5L fermenter, liquid volume 2.5L, 35°C, initial glycerol concentration 21g / L, rotation speed 150r / min, ventilation volume 0.5 L / min, add glycerol from 8h to keep it at about 21g / L, until 20h, use 10mol / L of KOH to control the pH and keep it at 8.0; meanwhile, mix the Klebsiella pneumoniae fermentation broth with the collected 5g / L (dry Heavy) Acetobacter sp. bacterial suspension mixed, the reaction conditions are: 35 ℃, pH5.5, after 48 hours of reaction, the output of 3-hydroxypropionic acid is 43-48g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing 3-hydroxypropionic acid by taking glycerin as a raw material fermentation-biological catalysis coupling synthesis system. The method comprises the following steps: fermenting a 1,3-propylene glycol producing strain (such as Klebsiella pnumoniae) and Acetobacter sp.) by taking glycerin as a substrate to produce 1,3-propylene glycol; catalyzing synthesis of 1,3-propylene glycol by taking a strain of selectively-oxidized polyhydric alcohol (such as Acetobacter sp. and gluconobacter oxydans) as a cell catalyst to obtain 3-hydroxypropionic acid; fermenting the Klebsiella pnumoniae by using a coupling reaction device, and performing resting cell catalytic reaction coupling with the Acetobacter sp.. A technology for preparing 3-hydroxypropionic acid by coupling 1,3-propylene glycol in an NADH (Nicotinamide Adenine Dinucleotide Hydrogen) reproducible way can be provided, and the method is very wide in the application prospect.

Description

technical field [0001] The invention relates to a method for microbial synthesis of 3-hydroxypropionic acid, in particular to a method for coupling and catalytically synthesizing 3-hydroxypropionic acid by using interspecies glycerol fermentation to produce 1,3-propanediol, belonging to the technical field of microbial fermentation and the field of biocatalysis . Background technique [0002] 3-Hydroxypropionic acid (3-HP) is an emerging platform compound, listed by the U.S. Department of Energy as one of the 12 most potential chemical products in the world. 3-Hydroxypropionic acid can generate 1,3-propanediol, malonic acid, acrylic acid, acrylonitrile and acrylamide through redox reaction, these compounds are necessary for the production of adhesives, polymer materials, fibers, plastics and resins The poly 3-hydroxypropionic acid produced by the polymerization of 3-hydroxypropionic acid has the advantages of high strength, good stretchability and good biodegradability, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P39/00C12P7/18C12P7/42C12R1/22C12R1/02
Inventor 诸葛斌宗红陆信曜方慧英诸葛健
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com