Method for producing 3-hydroxypropionic acid by taking glycerin as raw material fermentation-biological catalysis coupling synthesis system

Abstract

The invention discloses a method for producing 3-hydroxypropionic acid by taking glycerin as a raw material fermentation-biological catalysis coupling synthesis system. The method comprises the following steps: fermenting a 1,3-propylene glycol producing strain (such as Klebsiella pnumoniae) and Acetobacter sp.) by taking glycerin as a substrate to produce 1,3-propylene glycol; catalyzing synthesis of 1,3-propylene glycol by taking a strain of selectively-oxidized polyhydric alcohol (such as Acetobacter sp. and gluconobacter oxydans) as a cell catalyst to obtain 3-hydroxypropionic acid; fermenting the Klebsiella pnumoniae by using a coupling reaction device, and performing resting cell catalytic reaction coupling with the Acetobacter sp.. A technology for preparing 3-hydroxypropionic acid by coupling 1,3-propylene glycol in an NADH (Nicotinamide Adenine Dinucleotide Hydrogen) reproducible way can be provided, and the method is very wide in the application prospect.

Description

technical field

[0001] The invention relates to a method for microbial synthesis of 3-hydroxypropionic acid, in particular to a method for coupling and catalytically synthesizing 3-hydroxypropionic acid by using interspecies glycerol fermentation to produce 1,3-propanediol, belonging to the technical field of microbial fermentation and the field of biocatalysis . Background technique

[0002] 3-Hydroxypropionic acid (3-HP) is an emerging platform compound, listed by the U.S. Department of Energy as one of the 12 most potential chemical products in the world. 3-Hydroxypropionic acid can generate 1,3-propanediol, malonic acid, acrylic acid, acrylonitrile and acrylamide through redox reaction, these compounds are necessary for the production of adhesives, polymer materials, fibers, plastics and resins The poly 3-hydroxypropionic acid produced by the polymerization of 3-hydroxypropionic acid has the advantages of high strength, good stretchability and good biodegradability, and...