Active thermophilic bacterial strain and applications thereof

A technology of thermophilic microorganisms and biological emulsifiers, which is applied to bacteria, mining fluids, microorganisms, etc., can solve the problems such as the inability of microorganisms to grow normally, poor environmental adaptability, and unsatisfactory use effects, and achieve low production costs and adaptability. Strong, enhanced oil recovery effect

Active Publication Date: 2014-06-04
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

Moreover, the current strains of bioemulsifiers have the following problems in the application: 1. The activity is unstable, and most strains with strong activity under laboratory conditions cannot be applied to actual production; 2. The environmental adaptability is poor, and the actual environment is the same as the experiment The room conditions are too different, for exa

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  • Active thermophilic bacterial strain and applications thereof
  • Active thermophilic bacterial strain and applications thereof
  • Active thermophilic bacterial strain and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: the screening of biosurfactant bacterial strain

[0021] The activity characteristics of thermophilic microorganisms depend on the particularity of their growth environment. Oil-contaminated soil samples from the Yaerxia oil reservoir in Yumen Oilfield, Gansu, China were shaken and mixed with 5 times the volume of sterile saline, and 100 μl of soil was suspended. The turbid liquid was coated on a solid LB plate, and cultured in an incubator at 60°C for 3 days. Picked a single clone, and re-streaked on the LB plate for separation and purification until the purified single clone was obtained. It was found by Gram staining that the strain XS2 was Gram The strain XS2 was found to be short rod-shaped with spores by microscope observation; the semi-solid puncture inoculation test showed that the strain XS2 was facultative anaerobic, and the results of nitrate reduction and lipid hydrolysis tests were positive. Cellular DNA was extracted, and the 16S rRNA gene wa...

Embodiment 2

[0022] Example 2: Activity and emulsifier performance of bacterial strain Geobacillus sp.XS2

[0023] 1. Bacterial liquid preparation: LB plate was coated with seed liquid, and LB medium was prepared as follows (w / v): 1% peptone, 0.5% yeast extract, 1% sodium chloride, 2% agar powder added to solid medium, pH7 .5, place it upside down in a constant temperature incubator at 60°C and cultivate overnight. Pick a single colony, streak it onto a fresh LB plate, and repeat 2 to 3 times to obtain pure bacteria. Inoculate the strain in a 500mL Erlenmeyer flask containing 2% (w / v) crude oil inorganic salt medium, 180rpm, 60°C shaking culture, the inorganic salt formula is as follows: glucose 2%, sodium nitrate 0.3%, potassium dihydrogen phosphate 0.042 %, dipotassium hydrogen phosphate 0.12%, ferrous sulfate 0.005%, magnesium sulfate 0.05%, calcium chloride 0.005%, medium pH 7.5. The crude oil culture medium is that the glucose in the above-mentioned inorganic salt culture medium is ...

Embodiment 3

[0030] Embodiment 3: Optimum fermentation condition optimization of bacterial strain Geobacillus sp.XS2

[0031] 1. Screening of carbon and nitrogen sources. Strain XS2 can utilize various carbon sources such as sugars, organic acids, alcohols, lipids, and hydrocarbons for growth. When using glucose as a carbon source, the cell growth rate and emulsifying activity are the highest, and the emulsifier production is also the highest, as follows figure 1 shown. At the same time, the effects of 5 kinds of nitrogen sources on the synthetic emulsifier of the bacteria XS2 were screened as follows figure 2 It was shown that when sodium nitrate was used as nitrogen source, the emulsifying activity of fermentation broth and the output of emulsifier were the highest. Therefore, glucose and sodium nitrate were selected as the best carbon and nitrogen sources for the fermentation production of bioemulsifiers.

[0032] Further studies have proved that under the condition of 2% glucose, wh...

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Abstract

The invention provides a thermophilic microorganism that is bacillus licheniformis XS2 bacterial strain (Geobacillus pallidus). The accession number of the bacterial strain is CMCCC No.8338. The XS2 bacterial strain is used for production of an extracellular bioemulsifier by fermentation. The thermophilic bacterium XS2 has high resistance to pH, temperature, and salt components, and has wide adaptability for the petroleum polluted environment and the oil reservoir environment. The active bioemulsifier produced by the bacterial strain XS2 has advantages of high output, activity stability, high adaptability, relatively low production cost, and the like, can be used for enhancing oil recovery by microorganisms and repairing environment pollution and used for the chemical engineering industry, and has high development potential for broadening the application scope and increasing the efficiency of industrial bioemulsifiers in China.

Description

technical field [0001] The invention belongs to the technical field of functional microorganism screening and application, and specifically relates to a thermophilic bacterial strain and its application, that is, a bacterial strain capable of producing an active biological emulsifier, and the application of the bacterial strain converting glucose to secrete an extracellular biological emulsifier. Background technique [0002] Surfactant is a general term for amphiphilic molecular compounds that contain both hydrophilic and lipophilic groups and can significantly reduce the surface tension of solvents or liquid-liquid interfacial tension at very low concentrations. The structural characteristics determine the various physical and chemical properties of surfactants, such as amphiphilicity, solubility, surface adsorption, etc., and are widely used in petroleum industry, environmental engineering, food industry, leather, construction, cosmetics and other fields. [0003] Many mo...

Claims

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Application Information

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IPC IPC(8): C12N1/20E21B43/22B09C1/10C12R1/10
Inventor 黄志勇王兴彪徐爽王曼曼
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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