Preparation method of silk fibroin and sulfated silk fibroin composite tubular scaffold

Abstract

The invention provides a preparation method of a silk fibroin and sulfated silk fibroin composite tubular scaffold. The composite tubular scaffold is characterized in that the composite tubular scaffold is composed of an elastic bonding layer, a fabric reinforcing layer and a compact coating layer, and the fabric reinforcing layer is embedded in the elastic bonding layer, and the outer surface of the compact coating layer is covered with the elastic bonding layer, wherein the compact coating layer and the fabric enhancing layer are made of silk fibroin, and the elastic bonding layer is made a silk fibroin and sulfated silk fibroin mixture. The elastic bonding layer can improve the compliance and the anticoagulation property of the tubular scaffold, the fabric reinforcing layer can improve the mechanical properties of the scaffold, and the compact coating layer can improve the impermeability of the scaffold. The tubular scaffold prepared in the invention has excellent mechanical properties, has good impermeability, cell compatibility and blood compatibility, has a controllable diameter, and can be used for restoring and reconstructing small caliber vessels and constructing a hemodialysis access. The preparation method has the advantages of simple operation, low cost, and commercial production realization.

Description

(1) Technical field [0001] The invention belongs to the field of biomedical materials, in particular to a composite tubular scaffold of silk fibroin and sulfated silk fibroin with excellent mechanical properties, good permeability resistance, cell compatibility and blood compatibility. (2) Background technology [0002] In today's society, cardiovascular diseases seriously endanger human health, and vascular reconstruction plays a very important role in clinical surgery. More than 600,000 people around the world need to undergo various vascular surgeries every year, and most of them need suitable vascular grafts . At present, the vascular grafts used in clinical practice mainly include autologous blood vessels, allogeneic blood vessels and artificial synthetic blood vessels. Due to the limited source of autologous blood vessels and the rejection of allogeneic blood vessels, artificial vascular grafts have attracted much attention. At present, large-caliber artificial blood ...

AI Technical Summary

Problems solved by technology

Heparin is a common anticoagulant protein. Heparin can improve hemorheological properties after simple coating, but the binding strength is not enough, and it is easy to fall off, resulting in too fast release

In recent years, some scholars have used plasma irradiation to change the surface structure of materials, generate reactive groups on the surface of materials, and fix heparin on the surface of materials in the form of covalent bonds, but the anticoagulant effect is not satisfactory.

The scaffold material in the in vivo environment is exposed to various factors such as body fluids, organic macromolecules, enzymes, free radicals, cells, etc., and its biological environment is extremely complex. The method of surface modification can improve the long-term anticoagulant performance of the material Relatively limited, with the degradation of the stent material and the shedding of the surface modification layer, although its anticoagulant performance can meet the needs of large-caliber vessels with high blood flow and low resistance, it still cannot meet the requirements of small-caliber vessels with high anticoagulant performance. blood vessel needs

On the other hand, planting vascular endothelial cells on the inner surface of the scaffold material can form an anti-thrombotic surface layer, which can reduce the activity of prothrombin on the inner surface of tissue engineered blood vessels, thereby improving the hemocompatibility of the material, especially in small-diameter tissue engineered blood vessels. However, the insufficient preservation rate of endothelial cells after being washed by blood flow has always been a major problem for domestic and foreign scholars.

Researchers have published many techniques for preparing silk fibroin tubular scaffolds by braid reinforcement (Chinese Patent Application No. 201010249479.4, 201110135353.9). The mechanical properties of silk fibroin tubular scaffolds prepared by this method are greatly improved, but the blood phase Poor capacitance, which limits its further application

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