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A yeast-like fungus for suppressing postharvest diseases of fresh fruit and its application

A technology of yeast and fungi, applied in the direction of application, fungi, chemicals for biological control, etc., can solve problems such as shedding, peach damage, and economic loss of fruit farmers

Inactive Publication Date: 2016-10-05
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 2000, peach brown rot has increased in a large area in Beijing, and it has become more serious year by year. Among them, in the peach orchard with severe disease in 2005, peach fruits were damaged and a large number of peaches fell off. Fruits without obvious symptoms were picked. More than 50% of the fruits were picked 2-3 days after picking. of the fruits are rotted due to brown rot infection, which causes serious economic losses to the fruit growers
[0010] Pseudozyma fusiformata is widely distributed in nature, but there is no report on the control of Pseudozyma fusiformata in China at present

Method used

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  • A yeast-like fungus for suppressing postharvest diseases of fresh fruit and its application
  • A yeast-like fungus for suppressing postharvest diseases of fresh fruit and its application
  • A yeast-like fungus for suppressing postharvest diseases of fresh fruit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Isolation and strain identification of yeast-like fungi (Pseudozyma fusiformata) SF6 of the present invention

[0047] 1. Sample collection

[0048] Apples are collected from organic orchards in northern China.

[0049] 2. Isolation and screening of strains and antagonistic screening

[0050] Isolate strains from organic fruits, use conventional gradient dilution coating to isolate, use two kinds of media, PDA (containing streptomycin sulfate) and YPD agar (containing streptomycin sulfate), to culture at 28°C respectively, and pick colonies Bacteria with large morphological differences and can be purely cultured were purified and preserved on YPD agar medium, and the primary screening and multiple re-screening of antagonistic bacteria were carried out with Peach brown rot as the target pathogen, and finally a strain with strong antibacterial activity was obtained. Saccharomyces-like strain, named as SF6.

[0051] 3. Identification of strains

[0052] The ...

Embodiment 2

[0054] Physiological and biochemical characteristics of SF6: glucose can be fermented, raffinose and lactose can not be fermented; carbon source assimilation: sorbose, sucrose, maltose, trehalose, D-mannitol are positive, galactose, lactose, rhamnose , ribitol, and erythritol were negative. SF6 can grow normally in the medium that contains 5% (mass content) sodium chloride, can not grow in the medium that contains 10% (mass content) sodium chloride, can grow in the medium of pH4.0-5.5 It can grow normally, but the growth potential is weak when the ambient temperature is 4°C, and it cannot grow above 40°C; the optimum growth temperature is 25-30°C. The 5.8S rDNA gene, ITS1 / ITS2 sequence and 26S rDNA D1 / D2 nucleotide sequence of strain SF6 are shown in SEQ ID No.1 and No.2. Embodiment 2, the mensuration of class yeast fungus (Pseudozyma fusiformata) SF6 antibacterial spectrum of the present invention

[0055] Pathogens tested:

[0056] Peach brown rot fungus - Monilinia fruct...

Embodiment 3

[0065] Example 3 Inhibition of yeast fungus (Pseudozyma fusiformata) SF6 on rot lesions caused by two kinds of plant pathogenic fungi tested.

[0066] After the peaches were picked, they were passed through the SF6 bacterial solution (the concentration of the bacterial solution was 10 7 cfu / ml, 10 8 cfu / ml, 10 9 cfu / ml) for 30-60 seconds, then air-dried at room temperature, and then stored in the dark at a temperature of 1°C and a relative humidity of 95%. After storage for 21 days, compared with the control group, when the bacterial solution concentration was 10 7 cfu / ml, its control effect is 50%, when the concentration of bacteria solution reaches 10 8 When cfu / ml, its control effect is 60%, when the concentration of bacteria solution reaches 10 9 When cfu / ml, its control effect reaches 70%.

[0067] After the tomatoes were picked, the SF6 bacterial liquid (the concentration of the bacterial liquid was 10 7 cfu / ml, 10 8 cfu / ml, 10 9 cfu / ml) soaked for 30-60 seconds,...

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Abstract

The invention discloses pseudozyma fusiformata for inhibiting diseases after fresh fruit collection and application thereof, belonging to the technical field of microorganism application. The pseudozyma fusiformata SF6 disclosed by the invention has the preservation number of CGMCC No.8577, has inhibiting effects on monilinia after peach collection and botrytis cinerea after tomato collection, has inhibiting effects on the brown rot after peach collection and the grey mould after tomato collection, and has the most obvious inhibiting effect on the monilia, and the diameter of the inhibition zone is more than 15mm. The pseudozyma fusiformata has stable, efficient and broad-spectrum antimicrobial properties, and can be applied to prevention and treatment of the germs or diseases.

Description

technical field [0001] The invention relates to a yeast-like fungus for suppressing postharvest diseases of fresh fruits and an application thereof, belonging to the technical field of microbial application. Background technique [0002] Postharvest fungal diseases of fruits are an important class of diseases, which cause a large reduction in fruit yield, seriously affect the edible value and market value of fruits, and lead to a decrease in the economic income of fruit farmers. In developed countries, the losses caused by post-harvest diseases account for more than 15% of the total amount of fruits. In developing countries, due to the lack of necessary prevention and control technology and equipment, the losses caused by post-harvest diseases account for more than 30% of the total. . The occurrence of postharvest fruit diseases has seriously affected modern agricultural production, especially the production and processing of high-end fruit and vegetable products. [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 张殿朋刘伟成卢彩鸽刘霆刘德文张涛涛董丹吴慧玲裘季燕
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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