Inscribe chondroitin sulfate/dermatan sulfate 4-O-sulfatase as well as encoding gene and application of inscribe chondroitin sulfate/dermatan sulfate 4-O-sulfatase

A technology of sulfatase and encoding gene, which is applied in the field of genetic engineering to achieve the effects of repairing brain nerve damage, high endosulfatase activity and regulating metabolism
CN103952384AActive Publication Date: 2014-07-30SHANDONG UNIV
1 Cites 8 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2014-07-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to inscribe chondroitin sulfate / dermatan sulfate 4-O-sulfatase as well as an encoding gene and an application of the inscribe chondroitin sulfate / dermatan sulfate 4-O-sulfatase. The invention firstly discloses an amino acid sequence of the inscribe chondroitin sulfate / dermatan sulfate 4-O-sulfatase and a nucleotide sequence of the encoding gene of the inscribe chondroitin sulfate / dermatan sulfate 4-O-sulfatase. Found through detection, the enzyme has relatively high inscribe sulfatase activity, plays a role in removing sulfate radicals at the terminal and the inside of polysaccharide and can be effectively acted on polysaccharide, used for modifying and regulating the structure inside polysaccharide and applied to preparation of drugs for restoring cranial nerve involvement, regulating metabolism and treating inflammation and cancer.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to an endo-type chondroitin sulfate / dermatan sulfate 4-O-sulfatase and its coding gene and application, belonging to the technical field of genetic engineering. Background technique

[0002] Chondroitin Sulfate (CS) is a straight disaccharide unit composed of D-glucuronic acid and N-acetylgalactosamine via α-1,3-glycosidic bonds and repeated β-1,4-glycosidic bonds. chain polysaccharides. In the process of sugar chain synthesis, the D-glucuronic acid of chondroitin sulfate is usually converted into L-iduronic acid under the action of C5-epimerase, thus forming dermatan sulfate (Dermatan Sulfate, DS ), so chondroitin sulfate and dermatan sulfate regions often exist alternately in the sugar chain, forming a complex hybrid structure CS / DS. One of the main reasons for the highly complex structure of CS / DS is due to the sulfation of hydroxyl groups (-OH) at different positions in the sugar chain. CS synthesized in organisms in nature ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More