Culture medium and culture method for cultivating Tetraselmis subcordiformis by utilizing winery wastewater

Abstract

The invention discloses a method for cultivating Tetraselmis subcordiformis by utilizing winery wastewater. The technique can treat wine wastewater at low cost, change wastes into valuable substances, and obtain Tetraselmis subcordiformis with important bait value, thereby implementing multiple purposes. The traditional Tetraselmis subcordiformis culture solution has fewer nutrients and uses an open cement track tank for culture, and thus, has the defects of low growth speed, low yield, high investment, high tendency to pollution and low benefit. At present, a water tower made from high-density polyethylene with the volume of 3 cubic meters, which has the advantages of low cost and low tendency to pollution, can be reutilized and is easy to operate, is adopted; and wine wastewater, urea sea mud extracting solution, soil extracting solution, cattle manure leaching solution, L-cysteine, casein peptone, medical stone powder, sodium dihydrogen phosphate, S3 vitamin, human urine and other nutritional ingredients are added into the culture medium, and the organic fertilizer and inorganic fertilizer are mixed for use, so that the nutrition is more comprehensive and balanced, thereby greatly enhancing the growth speed of the Tetraselmis subcordiformis and enhancing the yield by 350%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a culture medium and a cultivation method for cultivating Tetraphyllum subcardiac by utilizing waste water from a winery. Background technique [0002] Wineries discharge large amounts of wastewater every year, especially during the fermentation season. Wastewater mainly comes from equipment (tank, pool, bottle, etc.) washing water, workshop washing water, and grape pomace, grape puree, and wine grains discharged during the production process. At the same time, brandy is the distilled spirit of wine. Many wineries have brandy production workshops, and the waste water mainly comes from distillation raffinate and washing water. During fermentation, the discharged wastewater contains sugar, organic acids, alcohol, and polyphenols. These organic matter decay will consume oxygen in the water. If the wastewater is discharged directly into the surrounding environment, the skin dr...

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Problems solved by technology

It can not only effectively remove suspended solids in wastewater, but also remove some organic matter. The removal rate of COD can reach 40%, and the removal rate of p(SS) can reach 50%. As a fuel, it can make up for part of the expenses required for operation, but there is secondary pollution. For example, a winery in Hebei adopts anaerobic method, with an investment of 600,000 yuan in equipment and infrastructure, a daily treatment capacity of 200 tons, and a COD value of wastewater at the entrance of 50,000mg / L. , but the COD value after treatment is still as high as 2000mg / L, which exceeds the emission standard by 20 times, and a fine of 50,000 yuan is imposed every year. To solve the secondary pollution, biochemical treatment must be carried out. The equipment needs at least 500,000 yuan, which is too much investment.

Some wine manufacturers also add various flocculants such as polyaluminum chloride, polyaluminum ferric chloride, basic aluminum chloride, aluminum sulfate, ferrous sulfate, polyaluminum ferric chloride, ferric chloride, and ferric sulfate to wastewater. , inorganic iron salt, coagulant polyacrylamide, etc., the water purification effect is also good, but the flocculant and water purifier are expensive, the cost is too large, and it is difficult to apply in production practice

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