Method for preparing n-butyl alcohol in fermentation mode by hydrolyzing lignocellulose through mixed cellulose crude enzyme

Abstract

The invention relates to a method for preparing n-butyl alcohol, in particular to a method for preparing n-butyl alcohol in a fermentation mode by hydrolyzing lignocellulose through mixed cellulose crude enzymes. According to the method, the problems that in the existing process that the n-butyl alcohol is produced by biological fermentation, a substrate is high in cost, the production cost is increased due to the use of commercial enzymes in the hydrolytic process of the lignocellulose, and the enzyme activity is reduced due to an unbalanced proportion of a cellulose system of the single kind of culture are solved. The method comprises the steps that the preprocessed lignocellulose is applied to the mixed cellulase crude enzymes of trichoderma viride and aspergillus niger and hydrolysis is conducted according to a biological enzyme method; after a nitrogen source, inorganic salt and vitamins are added to obtained liquid glucose, nitrogen is added to the liquid for deoxygenization; a clostridium acetobutylicum seed solution is inoculated for anaerobic fermentation, and therefore the n-butyl alcohol is generated. According to the method, the activity of biological enzymes is improved in the cellulose enzymolysis process, cost of the substrate and the enzymatic hydrolysis in the process that the n-butyl alcohol is produced by biological fermentation is reduced, the problems of device losses and environment pollution are solved, and the saccharity hydrolysis efficiency of the mixed crude enzymes reaches 75%.

Description

technical field [0001] The invention relates to a method for producing n-butanol by hydrolyzing lignocellulose and fermenting mixed cellulase crude enzyme liquid. Background technique [0002] Currently, the world's energy needs are largely met by fossil fuels. However, the depletion of fossil fuel resources has always been an important factor that plagues the sustainable development of human society. With the development of science and technology, human beings are increasingly dependent on energy for production and living activities. The demand for resources is increasing day by day, therefore, it is imperative to develop a new type of energy. As a new type of energy carrier, n-butanol has good miscibility with gasoline and can be directly applied to existing engines. It has the advantages of high energy density, octane number close to gasoline, and poor hydrophilicity, which is convenient for transportation. , known as a new generation of bioenergy. There are many ways ...

AI Technical Summary

Problems solved by technology

[0005] The invention aims to solve the problems of high substrate cost in the existing biological fermentation process of n-butanol production, increased cost caused by the application of commercial enzymes in the lignocellulose hydrolysis process, and reduced enzyme activity caused by the unbalanced ratio of a single strain of cellulosic enzymes.