Method for preparing diosgenin by virtue of homogenization process synergetic enzymatic hydrolysis

A technology of diosgenin and homogenate method, which is applied in the fields of steroids, fermentation, organic chemistry, etc., can solve the problems of high energy consumption, long production cycle, low extraction rate, etc., to increase the diffusion area and improve the yield , the effect of reducing resistance

Inactive Publication Date: 2014-10-01
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method requires repeated extraction and concentration, high energy consumption, long production cycle, low extraction rate, and serious pollution to the environment; while supercritical fluid extraction and ultrasonic extraction are effective, but There is a shortcoming of high one-time inve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0010] Example 1:

[0011] The impurity-removing slices of Panzanosaurus were dried, dried and crushed, passed through a 100-mesh sieve, 100g was taken, 50% ethanol was added 5 times (weight ratio) of the raw materials, and the powder was treated with a high-speed homogenizer at a power of 200W. Treated for 8 minutes to obtain the dioscin extract, and then add 3% (weight ratio) of the compound enzyme of the raw material (the ratio of cellulase, hemicellulase and amylase is 0.4:0.2:0.4), adjust the pH to 3.5 by acetic acid / sodium acetate , Stir evenly, enzymatically digest at 50°C for 2h, inactivate at 80°C for 15min, adjust the pH to neutral with sodium hydroxide, centrifuge, concentrate, and recrystallize with petroleum ether to obtain 1.92g diosgenin. After testing, the yield of diosgenin The rate is 1.92%.

Example Embodiment

[0012] Example 2:

[0013] The Ptereophyllum slicing slices were dried, dried and crushed, passed through a 100-mesh sieve, 100g was taken, and 70% ethanol of 7 times (weight ratio) of the raw material was added. A high-speed homogenizer was used to process the powder of Ptereophyllum at a power of 200W. Treated for 10 minutes to obtain the dioscin extract, and then add 4% (weight ratio) of the compound enzyme of the raw material (the ratio of cellulase, hemicellulase and amylase is 0.4:0.2:0.4), adjust the pH to 4.5 with acetic acid / sodium acetate , Stir evenly, enzymatically digest at 55°C for 1.5h, inactivate at 80°C for 15min, adjust the pH to neutral with sodium hydroxide, centrifuge, concentrate and recrystallize with ethanol to obtain 2.05g of diosgenin. After testing, the yield of diosgenin The rate is 2.05%.

Example Embodiment

[0014] Example 3:

[0015] The impurity-removing slices of Panzanosaurus were dried, dried and crushed, passed through a 100-mesh sieve, 100g was taken, 65% ethanol was added 8 times (weight ratio) of the raw materials, and the powder was treated with a high-speed homogenizer at a power of 200W. After 9 minutes of treatment, the diosgenin extract is obtained, and 3.5% (weight ratio) of the compound enzyme of the raw material (the ratio of cellulase, hemicellulase and amylase is 0.4:0.2:0.4) is added, and the pH of acetic acid / sodium acetate is adjusted to 4.0 , Stir evenly, enzymatically digest at 60°C for 1 hour, inactivate at 80°C for 15 minutes, adjust the pH to neutral with sodium hydroxide, centrifuge, concentrate, and recrystallize with methanol to obtain 1.97 g of diosgenin. After testing, the yield of diosgenin It is 1.97%.

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Abstract

The invention relates to a method for extracting diosgenin by virtue of homogenization process synergetic enzymatic hydrolysis. The method comprises the following treatment steps: removing impurities of ningpo yam rhizome, cutting into slices, drying, baking and crushing, screening by using a 100-mesh sieve, adding 50-70% ethanol which accounts for 5-8 times of the weight of a raw material, using a high-speed pulp refiner, treating ningpo yam rhizome powder for 8-10 minutes at a power of 200W to obtain a dioscin extracting solution, and then performing enzymatic hydrolysis, inactivation, centrifugation and re-crystallization to obtain diosgenin. The method disclosed by the invention is used for extracting diosgenin by adopting homogenization process synergetic enzymatic hydrolysis, is simple in process, short in extraction time and high in extraction rate, does not need expensive instruments, is low in production cost, and can be used for industrial production.

Description

technical field [0001] The invention belongs to the field of separation of effective components of natural products, and in particular relates to a method for extracting diosgenin by means of a homogenate method and cooperative enzymatic hydrolysis. Background technique [0002] Pangolin is the dried rhizome of Dioscorea pangolin, which contains diosgenin as the main component. There are more than 60 species of Dioscorea plant resources in my country, most of which contain a large amount of diosgenin. Diosgenin is hydrolyzed by acid or enzyme to generate diosgenin (commonly known as diosgenin), 1 molecule of glucose and 2 molecules of rhamnose. Diosgenin is a white crystal with a melting point of 204-207°C. It is soluble in commonly used organic solvents such as ether, petroleum ether, gasoline and acetic acid, but insoluble in water. It is an important synthetic raw material for the synthesis of various steroid drugs. [0003] At present, there are many patents and litera...

Claims

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Application Information

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IPC IPC(8): C12P33/20C07J71/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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