Mutagenesis method for tetraploid petunia

A petunia and tetraploid technology, which is applied in the field of tetraploid petunia mutagenesis, can solve the problems of expensive instruments, great influence on plant growth and high mortality of seedlings, and achieves the advantages of reducing the use concentration and improving the induction rate. Effect

Active Publication Date: 2014-11-26
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

[0007] The existing colchicine-induced polyploid method often has high seedling mortality, low induction success rate (usually lower than 10%), and is easy to form chimeric plants
Cytological identification of mutagenic plant ploidy generally takes the root tip as the detection object, a

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  • Mutagenesis method for tetraploid petunia
  • Mutagenesis method for tetraploid petunia
  • Mutagenesis method for tetraploid petunia

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Embodiment 1

[0027] Embodiment 1: a kind of mutagenesis method of tetraploid petunia,

[0028] 1 Materials and methods

[0029] 1.1 Materials Petunia 'Meilin' seeds are from Zhejiang Hongyue Flower Co., Ltd.

[0030] 1.2 Mutagenesis treatment

[0031] (1) Petunia 'Meilin' seeds are sown in plugs, and the matrix in the plugs is peat soil: perlite: pastoral soil mixed in a mass ratio of 6:1:1. When the two cotyledons of the seedlings are fully expanded, the The absorbent cotton is placed between the two cotyledons and is in close contact with the growth point.

[0032](2) Place the tray in the light incubator, set the light intensity to 4000lx, and the temperature is 30°C, and treat it at 8:00 am and 3:00 pm every day, and prepare it with dimethyl sulfoxide (DMSO) with a mass concentration of 1%. Pendimethalin (Pendimethalin) 6-60mg / L and colchicine mass concentration are 0.1%-1% mixed medicinal solution dripped on the absorbent cotton attached between the 2 cotyledons to ensure that the ...

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Abstract

The invention discloses a mutagenesis method for tetraploid petunia. The method comprises the following steps: dripping a mixed liquid medicine of Pendimethalin and colchicine to a growing point attached between two cotyledons in the process of allowing two cotyledons of diploid petunia to completely expand, and performing temperature change treatment; comparing the treated plants with contrast, selecting plants of which multiple agronomic traits such as leaf increase, leaf thickening, stem enlargement, plant height increase and column cap, anther and sepal enlargement change and of which anatomy traits such as leaf back air holes and pollen grains are obviously enlarged as variant plants; and finally, performing cytological identification, collecting small flower buds of the variant plants, observing metaphase of mitosis of pistil column cap body cells, and selecting a plant of which the chromosome number 2n is equal to 28 as a tetraploid plant. The method is short in treatment time, high in mutagenesis efficiency and simple and feasible in operation and has good application prospects, and the requirement of the identification method on instruments is low.

Description

technical field [0001] The invention belongs to the field of horticultural plant breeding, and in particular relates to a method for mutagenesis of tetraploid petunias. Background technique [0002] Plant polyploidy means that there are three or more chromosome groups in somatic cells. It is widely used because of its hugeness, strong stress resistance, low fruitiness and high nutritional content. Polyploidy can not only make plants The gene activity and the difference of enzymes are enhanced, and it also enhances the ecological adaptability of the plant, the resistance to adversity and the photosynthetic efficiency, etc., and promotes the growth of the plant, certain biological output, nutrient content and quality. . The study of polyploidy in plants is of great significance. It not only provides a lot of evidence for plant evolution, but also has wide application value in production practice and has broad prospects in modern breeding. [0003] Many plants can produce tet...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 魏跃嵇怡樊开青刘艳张莹史红林陈啸寅颜志明王全智贾思振董慧
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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