Pseudomonas fluorescens and application thereof in aquaculture

A technology of Pseudomonas fluorescens and breeding wastewater, which is applied in the field of Pseudomonas fluorescens, can solve the problems that the results cannot meet the actual needs of industrial production, and achieve remarkable results and broad application prospects

Active Publication Date: 2014-11-26
青岛蔚蓝龙初生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, aerobic denitrification has attracted more and more people's attention, and there are many researches on the screening of ae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: Isolation, screening and identification of strains

[0012] 1. Sample

[0013] Water samples taken from the wastewater treatment tower of salmon recirculating aquaculture in Yantai Oriental Ocean Group;

[0014] 2. Aerobic denitrification medium:

[0015] name

Dosage

KNO 3

1.0g

K H 2 PO 4

1.0g

Fecl 2 ·6H 2 o

0.5g

CaCl 2 2H 2 o

0.2g

MgSO 4 ·7H 2 o

1.0g

sodium succinate

8.5g

Bromothymol blue (1% ethanol solution)

1.0g

agar

20.0g

pure water

Dilute to 1000mL

[0016] 3. Screening method

[0017] Dilute the water sample to an appropriate concentration using the gradient dilution method; take 0.1 mL of the diluted water sample and spread it evenly on the surface of the aerobic denitrification medium, place it in a constant temperature incubator, and cultivate it at 30°C for 2 to 3 days, then use an inoculation loop Pick a si...

Embodiment 2

[0054] Embodiment 2: Pseudomonas fluorescens HF-3 treatment aquaculture water experiment

[0055] Activate Pseudomonas fluorescens HF-3, expand the culture, and make about 10 billion / g bacterial powder through liquid fermentation and spray drying.

[0056] The experiment was carried out in a small shed shrimp culture pond in Rudong, Jiangsu Province. Three culture ponds with similar water quality indicators were selected as three parallels, and the water bodies in the culture ponds were separated by plastic sheets. Half of the water bodies were used as the experimental group, and the other half as the experimental group. control group. Firstly, the NO in the experimental group and the control group were detected respectively. 2 - - the initial concentration of N, and then add 10ppm of the above-mentioned Pseudomonas fluorescens HF-3 bacteria powder to the water body of the experimental group, and the control group does not add it. During the experiment, the aeration was uni...

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PUM

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Abstract

The invention relates to the technical field of functional microbial screening, and in particular provides a Pseudomonas fluorescens HF-3 strain with the preservation number of CCTCC NO:M2014274. The strain has the aerobic denitrification property, and can efficiently degrade nitrite nitrogen in aquaculture sewage, 48h after the treatment, the removal rate of the nitrite nitrogen in the water reaches 94.61%, and the effect is remarkable; the Pseudomonas fluorescens HF-3 strain can be used for directly processing water in a shed shrimp aquaculture pool, and 48h after the treatment, about 94.35% of the nitrite nitrogen can be removed. The Pseudomonas fluorescens HF-3 strain can effectively degrade the nitrite nitrogen in the domestic wastewater, and the removal rate is higher than 90%, so that the Pseudomonas fluorescens HF-3 strain can be developed into aquaculture probiotics and sewage treatment agents, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a Pseudomonas fluorescens with aerobic denitrification function and its application. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly, and the scale of intensive and high-density farming has been expanding day by day. Concentrated feeding has resulted in the accumulation of a large amount of residual bait, feces, and animal and plant corpses in the aquaculture water body, resulting in a serious excess of nitrogen content in the water body and eutrophication. The aggravation of globalization has caused the destruction of the aquaculture ecological environment, frequent occurrence of fish and shrimp diseases, and ultimately caused huge economic losses. The nitrification process converts NO 2 - -N converted to NO 3 - -N is an important part of the nitrogen cycle in nature. With the development of hi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/39
Inventor 武玉强苟万里刘广
Owner 青岛蔚蓝龙初生物科技有限公司
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