Quantum dot fluorescent aspirin imprinted sensor and its preparation method and use

Abstract

The invention provides a quantum dot fluorescent aspirin imprinted sensor and its preparation method and use and belongs to the technical field of material preparation and drug content detection. The preparation method comprises the following steps of preparing a precursor NaHTe solution from sodium borohydride, tellurium powder and water in an ultrasonic environment, injecting the precursor NaHTe solution into a CdCl2.2.5H2O solution fed with nitrogen for oxygen removal, having a pH value of 10.5-11.5 and containing thioglycollic acid (TGA), carrying out backflow reaction processes in a nitrogen protective atmosphere at a temperature of 100-110 DEG C to obtain quantum dots having different sizes according to different backflow time periods, and synthesizing a fluorescent molecule imprinted polymer from the CdTe quantum dots as fluorescent carriers, aspirin as a template molecule, (3-aminopropyl)triethoxysilane (APTES) as a functional monomer and tetraethyl orthosilicate (TEOS) as a cross-linking agent by a sol-gel method, wherein the fluorescent molecule imprinted polymer can be used for optical detection of aspirin. The fluorescent molecule imprinted polymer has good optical and pH stability and has an aspirin selective-identification function.

Description

technical field

[0001] The invention relates to a quantum dot fluorescent aspirin imprint sensor and a preparation method and application thereof, belonging to the technical field of material preparation and drug content detection. Background technique

[0002] Aspirin (aspirin), also known as acetylsalicylic acid (acetylsalicylic acid), is the earliest, most widely used and most common antipyretic and analgesic antirheumatic drugs. It has various pharmacological effects such as antipyretic, analgesic, anti-inflammatory, anti-rheumatic and anti-platelet aggregation, etc. Arthritis and toothache etc. It has the characteristics of rapid drug effect, stable drug effect, and easy diagnosis and treatment of overdose. However, with the widespread use of aspirin, its adverse reactions are gradually increasing. The more common symptoms are nausea, vomiting, upper abdominal discomfort or pain, etc., so it is very important to monitor the amount of aspirin. At present, chromatograp...

Examples

Embodiment 1

[0031] (1) 30.3 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0032] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 10.5 for nitrogen and deoxygenation 2 In aqueous solution, to which 228.34 mg of CdCl was added 2 2.5H 2 O and 138.5 μL of TGA. Mixed solution under nitrogen protection 100 o C under reflux reaction for 60 hours to obtain red fluorescent quantum dots.

[0033] (3) Mix 3 mL of quantum dot stock solution and 10 mL of twice distilled water into the flask, add 11.7 μL of (3-aminopropyl)triethoxysilane and 4.5 mg of aspirin at the same time, and stir for 20 min. Then 44.8 μ...

Embodiment 2

[0036] (1) 60.6 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0037] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 11.5 for nitrogen and deoxygenation 2 in aqueous solution to which 152.23 mg of CdCl was added 2 2.5H 2 O and 115.44 μL of TGA. The mixed solution was protected under nitrogen at 110 o C under reflux reaction for 60 hours to obtain red fluorescent quantum dots.

[0038] (3) Mix 0.1 mL of quantum dot stock solution and 10 mL of twice distilled water into the flask, add 70.2 μL (3-aminopropyl)triethoxysilane and 4.5 mg of aspirin at the same time, and stir for 30 min. Th...

Embodiment 3

[0041] (1) Add 45.4 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0042] (2) Inject the newly obtained precursor NaHTe into CdCl with thioglycolic acid (TGA) at a pH of 11.2 for nitrogen and deoxygenation 2 in aqueous solution to which 182.672 mg of CdCl was added 2 2.5H 2 O and 133 μL of TGA. Mixed solution under nitrogen protection 105 o C under reflux reaction for 60 hours to obtain red fluorescent quantum dots.

[0043] (3) Mix 2 mL of quantum dot stock solution and 10 mL of twice distilled water into the flask, add 23.4 μL of (3-aminopropyl)triethoxysilane and 4.5 mg of aspirin at the same time, and stir for 25 min. Then 112 μL t...

technical field

[0001] The invention relates to a quantum dot fluorescent aspirin imprint sensor and a preparation method and application thereof, belonging to the technical field of material preparation and drug content detection. Background technique

[0002] Aspirin (aspirin), also known as acetylsalicylic acid (acetylsalicylic acid), is the earliest, most widely used and most common antipyretic and analgesic antirheumatic drugs. It has various pharmacological effects such as antipyretic, analgesic, anti-inflammatory, anti-rheumatic and anti-platelet aggregation, etc. Arthritis and toothache etc. It has the characteristics of rapid drug effect, stable drug effect, and easy diagnosis and treatment of overdose. However, with the widespread use of aspirin, its adverse reactions are gradually increasing. The more common symptoms are nausea, vomiting, upper abdominal discomfort or pain, etc., so it is very important to monitor the amount of aspirin. At present, chromatograp...

Examples

Embodiment 1

[0031] (1) 30.3 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0032] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 10.5 for nitrogen and deoxygenation 2 In aqueous solution, to which 228.34 mg of CdCl was added 2 2.5H 2 O and 138.5 μL of TGA. Mixed solution under nitrogen protection 100 o C under reflux reaction for 60 hours to obtain red fluorescent quantum dots.

[0033] (3) Mix 3 mL of quantum dot stock solution and 10 mL of twice distilled water into the flask, add 11.7 μL of (3-aminopropyl)triethoxysilane and 4.5 mg of aspirin at the same time, and stir for 20 min. Then 44.8 μ...

Embodiment 2

[0036] (1) 60.6 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0037] (2) Inject the newly obtained precursor NaHTe into the CdCl with thioglycolic acid (TGA) at a pH of 11.5 for nitrogen and deoxygenation 2 in aqueous solution to which 152.23 mg of CdCl was added 2 2.5H 2 O and 115.44 μL of TGA. The mixed solution was protected under nitrogen at 110 o C under reflux reaction for 60 hours to obtain red fluorescent quantum dots.

[0038] (3) Mix 0.1 mL of quantum dot stock solution and 10 mL of twice distilled water into the flask, add 70.2 μL (3-aminopropyl)triethoxysilane and 4.5 mg of aspirin at the same time, and stir for 30 min. Th...

Embodiment 3

[0041] (1) Add 45.4 mg sodium borohydride (NaBH 4 ) and 51.04 mg of tellurium powder were added to the centrifuge tube, and then 3.0 mL of twice-distilled water was added to dissolve the solid completely; the centrifuge tube was placed in an ultrasonic machine for ultrasonic reaction, and the mouth of the tube was kept to vent gas, and the final white liquid was the desired The precursor NaHTe solution.

[0042] (2) Inject the newly obtained precursor NaHTe into CdCl with thioglycolic acid (TGA) at a pH of 11.2 for nitrogen and deoxygenation 2 in aqueous solution to which 182.672 mg of CdCl was added 2 2.5H 2 O and 133 μL of TGA. Mixed solution under nitrogen protection 105 o C under reflux reaction for 60 hours to obtain red fluorescent quantum dots.

[0043] (3) Mix 2 mL of quantum dot stock solution and 10 mL of twice distilled water into the flask, add 23.4 μL of (3-aminopropyl)triethoxysilane and 4.5 mg of aspirin at the same time, and stir for 25 min. Then 112 μL t...

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