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A method for the synthesis of structured lipids based on enzymatic acid hydrolysis of palmitic triglycerides

A palmitic acid triglyceride, structured lipid technology, applied in the fields of edible oil/fat, food science, application, etc., can solve the problems of waste of resources, the effect of the preparation process is not achieved, the preparation process is cumbersome, etc., and can extend the industrial chain. , the effect of improving resource utilization

Active Publication Date: 2016-09-14
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, due to the differences in the geographical distribution of raw materials and the huge differences in the distribution and content of each fatty acid in oil triglycerides, as well as the limitations of factors such as religious beliefs, cultural differences, and dietary preferences, and the cumbersome preparation process, the effect of the preparation process is not good. Meet people's expectations, resulting in a waste of resources

Method used

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  • A method for the synthesis of structured lipids based on enzymatic acid hydrolysis of palmitic triglycerides
  • A method for the synthesis of structured lipids based on enzymatic acid hydrolysis of palmitic triglycerides
  • A method for the synthesis of structured lipids based on enzymatic acid hydrolysis of palmitic triglycerides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This example illustrates the process of extracting free fatty acids from different raw oils and fats, and the obtained free fatty acids are used in subsequent examples.

[0037] Alkaline hydrolysis is adopted; the free fatty acid extraction method is: dissolve the oil in 95vt.% ethanol of 0.006g / mL NaOH, the oil concentration is 0.25g / mL, saponify and reflux in nitrogen (65°C, 6h), and recover by rotary evaporation Ethanol, add 50mL of distilled water and adjust the pH to 3~4 with HCl, let it stand for 1h, add 50mL of petroleum ether after removing water, then add 50mL of distilled water and wash until the oil phase is neutral, take the petroleum ether layer and dehydrate with anhydrous magnesium sulfate After filtration and rotary evaporation, free fatty acids were obtained. After methyl esterification, the types and contents of fatty acids in each component were detected by gas chromatography. The results are shown in Table 1.

[0038] Table 1. Types and contents of...

Embodiment 2

[0041] This example illustrates the reaction process of enzymatic acid hydrolysis of free fatty acid and palmitic acid triglyceride.

[0042] Silkworm chrysalis oil was hydrolyzed with alkali to obtain free fatty acids, and enzymatic acid hydrolysis of silkworm chrysalis oil free fatty acids and palmitic acid triglycerides prepared sn-2 structural lipids rich in palmitic acid. Among them, the content of unsaturated fatty acid in silkworm chrysalis oil is more than 80%, and the content of oleic acid, linoleic acid and alpha-linolenic acid is more than 80%. Reaction conditions: substrate ratio is silkworm chrysalis oil free fatty acid (mol): palmitic acid triglyceride (mol)=12:1; Lipozyme RM IM enzyme amount: 10% (by substrate weight, wt%); Temperature: 60 ℃ ;Reaction time: 24h. After the reaction, samples were collected, and after methyl esterification, gas chromatography was used to determine the fatty acid species, content and distribution of the product triglyceride. As de...

Embodiment 3

[0046] This example illustrates the reaction process of enzymatic acid hydrolysis of free fatty acid and palmitic acid triglyceride.

[0047] Silkworm chrysalis oil was hydrolyzed with alkali to obtain free fatty acids, and enzymatic acid hydrolysis of silkworm chrysalis oil free fatty acids and palmitic acid triglycerides prepared sn-2 structural lipids rich in palmitic acid. Among them, the content of unsaturated fatty acid in silkworm chrysalis oil is more than 80%, and the content of oleic acid, linoleic acid and alpha-linolenic acid is more than 80%. Reaction conditions: substrate ratio is silkworm chrysalis oil free fatty acid (mol): palmitic acid triglyceride (mol)=7: 1; Lipozyme RM IM enzyme amount: 7% (by substrate weight, wt%); Temperature: 40 ℃ ; Reaction time: 48h. After the reaction, samples were collected, and after methyl esterification, gas chromatography was used to determine the fatty acid species, content and distribution of the product triglyceride. As de...

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Abstract

Based on the method of enzymatic acid hydrolysis of palmitic triglycerides to synthesize structural lipids, saponify and reflux the oil raw materials and solvents in nitrogen. After the hydrolysis is complete, the solvent is recovered by rotary evaporation, the pH value of the hydrolysis mixture is adjusted, and the free fatty acids are extracted after washing with water. After removing water from magnesium sulfate, filter, take the filtrate and spin steam to obtain free fatty acids; mix at least one free fatty acid with palmitic triglyceride, and prepare structural lipids under the action of sn‑1, 3 specific lipase . The invention uses sn-1, 3-position specific lipase to catalyze palmitic acid triglyceride and fatty acid to synthesize structural lipids. MLM-type structured lipids were prepared by enzymatic acid hydrolysis of short- and medium-chain saturated fatty acids and palmitic acid triglycerides, which are easy to digest and absorb. At the same time, LPL-type structured lipids are prepared by enzymatic acid hydrolysis of human essential long-chain unsaturated fatty acids and palmitic acid triglycerides. Different types of structured lipids can be configured according to the different nutritional needs of different groups of people.

Description

technical field [0001] The invention belongs to the technical field of oil bioprocessing, and in particular relates to a method for synthesizing structural lipids based on enzymatic acidolysis of palmitic acid triglyceride. Background technique [0002] As we all know, structured lipids (Structured lipids, SLs) are modified natural lipids, directional addition of short carbon chain fatty acids, medium carbon chain fatty acids, and long carbon chain unsaturated fatty acids, because of their special fatty acid composition and fatty acid in glycerol The specific position in the triester makes it have special physiological function and nutritional value. In recent years, there has been a strong link between high fat and coronary heart disease, obesity, and certain cancers. Fat plays a unique and important role in various foods: providing energy, providing essential fatty acids and promoting the absorption of fat-soluble vitamins and calcium (Journal of Agricultural and Food Che...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23D9/013A23D9/04
Inventor 王俊王旭东庞娜王超吴福安
Owner JIANGSU UNIV OF SCI & TECH
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