Application of WWW tripeptides in preparation of medicines for treating Alzheimer disease
A technology for Alzheimer's disease and drugs, applied in the field of medicine, can solve problems such as ineffective effects, unclear drugs for Alzheimer's disease, and large toxic and side effects of drugs
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experiment example 1
[0020] Experimental example 1, a kind of Thioflavin T test of WWW tripeptide for preventing or treating Alzheimer's disease:
[0021] Aβ 1-42 Dissolve in pH7.4, the concentration is 0.01mol / L in the phosphate buffer solution of 0.01mol / L and make 50μmol / L mixed solution, Aβ in this mixed solution 1-42 The initial concentration of the peptide was 50 μmol / L, until the final concentration was 2.5 μmol / L, placed at 37 ° C for 48 h, then added ThT (5 μmol / L dissolved in 50 mmol / L glycine-NaOH solution, pH 8.50 ) to detect fluorescence at 450 nm and 485 nm. Each sample was measured 3 times and the fluorescence intensity was recorded.
[0022] to combine figure 1 As shown, the results of Example 1 show that: Thioflavin T can rapidly interact with Aβ 1-42 Combined with the polymer fiber, a new light with a wavelength of 450nm is excited, and the light with a wavelength of 482nm is obviously enhanced, while WWW tripeptide and Aβ 1-42 Co-cultured WWW tripeptide-Aβ 1-42 The thiof...
experiment example 2
[0023] Experimental example 2, a circular dichroism test of WWW tripeptide for preventing or treating Alzheimer's disease:
[0024] Aβ at a concentration of 50 μmol / L 1-42 Dissolved in phosphate buffer solution, and added WWW tripeptide at a concentration of 50 μmol / L to the experimental group, and then incubated at 37°C for 48 h, the Aβ in the mixed solution 1-42 and the final concentration of WWW tripeptide is 5μmol / L, using a container dish with a thickness of 1mm, Aβ 1-42 Monomeric and WWW tripeptide-Aβ 1-42 The complex samples were respectively prevented from detecting the structure in a circular dichroism spectrometer, and the spectra were recorded at 25°C with a wavelength of 190-260 nm and a wave width of 0.5 nm.
[0025] to combine figure 2 As shown, the results of Example 2 show that the circular dichroism spectrum shows that the β sheet structure in the WWW tripeptide-Aβ complex will decrease, such as figure 2 As shown, circular dichroism detection of Aβ 1-...
experiment example 3
[0026] Experimental Example 3, a transmission electron microscope (TEM) test of a WWW tripeptide for preventing or treating Alzheimer's disease:
[0027] Aβ with phosphate buffer (PBS, pH7.4) 1-42 The sample was dissolved to a concentration of 1 mg / ml, and then placed in the WWW tripeptide experimental group and the blank control group at a concentration of 25 μmol / L, and incubated for 48 hours until the final concentration of the mixed solution was 25 μmol / L, and 5 μl of the sample was applied Add 1% uranyl formate to the 300-hole Formvar-carbon copper grid, stain for 1 min, dry in the air, and then observe under the electron microscope to detect Aβ 1-42 and WWW Tripeptide-Aβ 1-42 The structure of the complex.
[0028] to combine image 3 Shown, embodiment 3 result shows: as image 3 As shown in the electron microscope image, from Aβ 1-42 Monomer, high-density long linear Aβ can be observed in the figure 1-42 fibers, the fibers aggregate into parallel bundles, and th...
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