Use of www tripeptide in preparing medicine for treating Alzheimer's disease
A technology for Alzheimer's disease and drugs, applied in the field of medicine, can solve the problems of large drug side effects, ineffective effects, unclear drugs for Alzheimer's disease, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
experiment example 1
[0020] Experimental example 1, a kind of Thioflavin T test of WWW tripeptide for preventing or treating Alzheimer's disease:
[0021] Aβ 1-42 Dissolve in pH7.4, the concentration is 0.01mol / L in the phosphate buffer solution of 0.01mol / L and make 50μmol / L mixed solution, Aβ in this mixed solution 1-42 The initial concentration of the peptide was 50 μmol / L, and until the final concentration was 2.5 μmol / L, it was incubated at 37°C for 48 hours, and then ThT (5 μmol / L dissolved in 50 mmol / L glycine-NaOH solution, pH 8.50) was added for detection Fluorescence at 450nm and 485nm. Each sample was measured 3 times and the fluorescence intensity was recorded.
[0022] combine figure 1 As shown, the results of Example 1 show that: Thioflavin T can rapidly interact with Aβ 1-42 Combined with the polymer fiber, a new light with a wavelength of 450nm is excited, and the light with a wavelength of 482nm is obviously enhanced, while WWW tripeptide and Aβ 1-42 Co-cultured WWW tripeptid...
experiment example 2
[0023]Experimental example 2, a circular dichroism test of WWW tripeptide for preventing or treating Alzheimer's disease:
[0024] Aβ at a concentration of 50 μmol / L 1-42 Dissolve in phosphate buffer solution, and add WWW tripeptide with a concentration of 50μmol / L to the experimental group, then place it at 37°C and incubate for 48h, the Aβ in the mixed solution 1-42 and the final concentration of WWW tripeptide is 5μmol / L, using a container dish with a thickness of 1mm, Aβ 1-42 Monomeric and WWW tripeptide-Aβ 1-42 The complex samples were respectively prevented from detecting the structure in a circular dichroism spectrometer, and the spectra were recorded at 25°C, with a wavelength of 190-260nm and a wave width of 0.5nm.
[0025] combine figure 2 As shown, the results of Example 2 show that the circular dichroism spectrum shows that the β sheet structure in the WWW tripeptide-Aβ complex will decrease, such as figure 2 As shown, circular dichroism detection of Aβ 1-42...
experiment example 3
[0026] Experimental Example 3, a transmission electron microscope (TEM) test of a WWW tripeptide for preventing or treating Alzheimer's disease:
[0027] Aβ with phosphate buffer (PBS, pH7.4) 1-42 The sample was dissolved to a concentration of 1 mg / ml, and then placed in the WWW tripeptide experimental group and the blank control group at a concentration of 25 μmol / L until the final concentration of the mixed solution was 25 μmol / L. 300 wells of Formvar-carbon copper grid, stained with 1% uranyl formate for 1 min, dried in the air, and observed under an electron microscope to detect Aβ 1-42 and WWW Tripeptide-Aβ 1-42 The structure of the complex.
[0028] combine image 3 Shown, embodiment 3 result shows: as image 3 As shown in the electron microscope image, from Aβ 1-42 Monomer, high-density long linear Aβ can be observed in the figure 1-42 fibers, the fibers aggregate into parallel bundles, and the bundles cross each other; however, from WWW tripeptide-Aβ 1-42 Only a...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com