Medium of composite bacterium for culture treatment coking wastewater, and its application

A coking wastewater and culture medium technology, which is applied in natural water body treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem that the degradation efficiency cannot be further improved, the biophase composition on the surface of the filter material is complex, It is not easy to decompose by microorganisms and other problems, and achieves the effects of strong degradation ability of organic pollutants, good ammonia nitrogen removal effect and high biological activity

Active Publication Date: 2015-02-25
中国中化股份有限公司 +1
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AI Technical Summary

Problems solved by technology

Compared with physical and chemical methods, the biological treatment technology that uses the metabolism of microorganisms to remove organic pollutants in wastewater has the advantages of low cost, high efficiency, easy operation, and no secondary pollution. It is an inevitable trend in future development. There is no comparison at present. Biochemical method is more economical and effective
However, the common feature of coking wastewater is that it contains more biodegradable persistent pollutants, which are not easily decomposed by microorganisms in the existing biological treatment system, resulting in a large amount of accumulation in the environment; in addition, in the application of specific water treatment technologies , the biofilm naturally formed in the conventional process has low activity and quantity of biological flora, and the composition of the biological phase on the surface of the filter material is complex, it is difficult to form a highly active flora, and the degradation efficiency of some toxic and harmful organic substances cannot be further improved

Method used

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  • Medium of composite bacterium for culture treatment coking wastewater, and its application
  • Medium of composite bacterium for culture treatment coking wastewater, and its application
  • Medium of composite bacterium for culture treatment coking wastewater, and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of culture medium:

[0025] Medium Ⅰ: tryptone 10g, yeast extract 5g, NaCl 10g, water 1000mL, pH 7.0, sterilized at 121°C for 20min.

[0026] No. Ⅱ medium: 10g sucrose, 15g soluble starch, 5g glycerol, 2g fish meal, 8g urea, (NH 4 ) 2 SO 4 2g, NaCl3g, K 2 HPO 4 3.5g, KH 2 PO 4 1.5g, 1000mL of water, pH7.0, sterilized at 121°C for 20min.

[0027] The method for preparing the above-mentioned composite microbial agent, its steps are as follows:

[0028] 1) Preparation of composite bacteria: Pseudomonas, Acinetobacter, Bordetella, Klebsiella, Bacillus megaterium and Bacillus subtilis were inoculated in No. Ⅰ medium for cultivation at 120rpm / min Cultivate on a shaking table at 30°C for 24 hours, so that the concentration of each bacteria reaches 10 8 more than one / mL, and then the bacterial strains were mixed according to the percentage of the number, specifically, the percentage of the composite bacteria was calculated according to the percentage of the...

Embodiment 2

[0035] The above-mentioned compound bacterial agent prepared by the culture medium of Example 1 was added to different coking wastewater containing phenols, heterocyclics, and polycyclic aromatic hydrocarbons, and the addition amount was 0.5% of the weight of coking wastewater. The actual treatment effect is shown in The table shows that the removal rate of COD in wastewater can be improved to varying degrees after the addition of bacterial agents.

[0036] Table 2 Treatment effect of composite bacterial agents on actual coking wastewater

[0037]

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Abstract

The invention relates to technical field of microbial inoculant culture, and especially relates to a medium of a composite bacterium for culture treatment coking wastewater, and its application. The medium for enlarged culturing of the composite bacterium for culture treatment coking wastewater is a medium II; and pseudomonades, Acinetobacter, Bordetella sp., Klebsiella pneumoniae, Bacillus megaterium and Bacillus subtilis are cultured in the medium II to prepare a treatment coking wastewater inoculant in order to remove pollutants in the coking wastewater. The quantity of bacteria obtained by culturing the above mixed bacteria in the medium II is 1.5-2.5 times the quantity of bacteria obtained by using a conventional LB medium, and the cost of the medium II only accounts for 50-70% of the cost of the conventional LB medium.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agent cultivation, in particular to a medium for cultivating complex bacteria used for treating coking wastewater and its application. Background technique [0002] At present, China's coking wastewater has a large amount of discharge and high COD, which is a major pollution source causing water environmental pollution. Compared with physical and chemical methods, the biological treatment technology that uses the metabolism of microorganisms to remove organic pollutants in wastewater has the advantages of low cost, high efficiency, easy operation, and no secondary pollution. It is an inevitable trend in future development. There is no comparison at present. Biochemical methods are more economical and effective. However, the common feature of coking wastewater is that it contains more biodegradable persistent pollutants, which are not easily decomposed by microorganisms in the existing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/22C12R1/11C12R1/125C12R1/01
CPCC02F3/34C02F2103/34C12N1/20
Inventor 朱希坤戴速航李小明王芳孙鸿曼
Owner 中国中化股份有限公司
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