Method used for simultaneous preparation of phospholipid microtubule and vesicle using point-plane electrode electric field

A point-surface electrode and phospholipid technology, which is applied in the field of simultaneous preparation of phospholipid microtubules and vesicles, can solve the problems of not being able to prepare phospholipid microtubules and vesicles at the same time, and achieve high yield, long length, and mild reaction conditions.

Active Publication Date: 2015-04-01
宜兴环保产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing method cannot prepare phospholipid microtubules and vesicles at the same time, a method for simultaneously preparing phospholipid microtubules and vesicles using the electric field of a point-surface electrode

Method used

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  • Method used for simultaneous preparation of phospholipid microtubule and vesicle using point-plane electrode electric field
  • Method used for simultaneous preparation of phospholipid microtubule and vesicle using point-plane electrode electric field
  • Method used for simultaneous preparation of phospholipid microtubule and vesicle using point-plane electrode electric field

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specific Embodiment approach 1

[0017] Specific implementation mode one: combine figure 1 , this embodiment is a method for simultaneously preparing phospholipid microtubules and vesicles by using the electric field of a point-surface electrode, specifically, it is completed according to the following steps:

[0018] 1. Clean the electrode: use absolute ethanol and distilled water as cleaning agents to clean the ITO electrode in sequence, and then blow it dry with nitrogen to obtain the cleaned ITO electrode; soak the tungsten needle electrode in the chromic acid solution for 3h to 6h, and then Rinse with distilled water for 3 to 6 times, and then blow dry with nitrogen to obtain a cleaned tungsten needle electrode;

[0019] 2. Preparation of phospholipid dry film: Dissolve phospholipids in chloroform to obtain phospholipid / chloroform solution; then evenly coat 1 μL to 10 μL of phospholipid / chloroform solution on the conductive surface of the cleaned ITO electrode, and dry naturally at room temperature for 2...

specific Embodiment approach 2

[0028] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that: in step 1, use absolute ethanol and distilled water as cleaning agents to perform ultrasonic cleaning on the ITO electrode for 5 minutes to 20 minutes, and then dry it with nitrogen to obtain Cleaned ITO electrode. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0029] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the mass fraction of the chromic acid solution described in step 1 is 5-12%. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses to a method used for simultaneous preparation of phospholipid microtubule and vesicle using point-plane electrode electric field. The method is used for solving a problem that existing method is not capable of realizing simultaneous preparation of phospholipid microtubule and vesicle. The method comprises following steps: step 1, electrode cleaning; step 2, preparation of phospholipid dry membrane; step 3, assembling of a sealed preparation apparatus; and step 4, preparation of the phospholipid microtubule and vesicle, and obtaining of the phospholipid microtubule and vesicle. Advantages of the method are that: firstly, the method is capable of realizing simultaneous preparation of the phospholipid microtubule and vesicle using point-plane electrode electric field in a same system, form of the obtained phospholipid microtubule is good, yield is high, and phospholipid microtubule size is relatively large and uniform; and secondly, the length of the obtained phospholipid microtubule ranges from 200 to 1300 mum, and diameter ranges from 1 to 2 mum; and diameter of the obtained phospholipid vesicle ranges from 15 to 120 mum. The method is used for simultaneous preparation of the phospholipid microtubule and vesicle using point-plane electrode electric field.

Description

technical field [0001] The invention relates to a method for simultaneously preparing phospholipid microtubules and vesicles. Background technique [0002] Molecular self-assembly is ubiquitous in biological systems and is the basis for the formation of different complex biological structures. Phospholipid microtubules and phospholipid vesicles, as different forms of self-assembly of phospholipid molecules, have important research and applications in the fields of biology, engineering bionics, pharmacology, medicine, and the development of new materials. At present, the methods for preparing phospholipid microtubules mainly include water dispersion method, direct precipitation method, thermal cycle method and microfluidic method, etc., and the methods for preparing phospholipid vesicles mainly include extrusion method, film dispersion method, organic solvent dispersion method, and electroporation method. etc., but there is no method for the controllable preparation of phosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/08
CPCB01J13/02B01J19/087B01J2219/0803B01J2219/0807
Inventor 韩晓军朱春桃
Owner 宜兴环保产业有限公司
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