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Composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof

A technology to enhance immunity and syndrome, applied in the direction of application, drug combination, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2015-04-22
XIAMEN HONGJIE ENVIRONMENTAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immune enhancement effect of these substances is the result of investigation in the laboratory, and there is no result of the test in the farm

Method used

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  • Composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof
  • Composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof
  • Composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Investigation of the Immunological Activity of the Sample (DF) and the Palatability of the Sample

[0056] In order to explore natural substances that have an immune-enhancing effect on white spot syndrome infected with prawns, the effects of extracts of cereals, Chinese herbal medicines, and seaweed on the immune activity against prawns were investigated. In this experiment, prawns with an average weight of 10.49±0.63g and a body length of 110.89±2.99mm were put into a fish tank with a circulation filter, 10 each, and fed with feed added with various medicinal material extracts. After 10 days of breeding, The results of the blood collection and NBT (superoxide production) evaluation method, and grains are shown in figure 1 , Chinese herbal medicines are expressed in figure 2 , algae expressed in image 3 .

[0057] Cereals are provided with 9 kinds of extracts such as coix coix and barley, among which 6 substances with good immune effects are expressed i...

Embodiment 2

[0065] Manufacture of Example 2 sample (DF)

[0066]Collect natural substances, 6 kinds of grains: corn, barley, coix, millet, buckwheat, wheat (Zhaoan, Fujian Province), 25 kinds of Chinese herbal medicines: Divine Comedy, malt, rhubarb, Huoxiang, licorice, perilla, Atractylodes macrocephala, Chuanxiong, Scutellaria baicalensis, three white grass, hawthorn, angelica, red ginseng, medlar, berberine, astragalus, schisandra, cassia, artemisia annua, Poria cocos, Chuanpu, dogwood, woody, akebia, forsythia (Zhaoan, Fujian Province ), 4 kinds of seaweed: kelp, sargassum, pine algae, and seaweed (Zhaoan, Fujian Province) are used as objects. After extraction by hot water extraction and organic solvent extraction such as ethanol, feed is added. In order to eliminate heavy metals, the selected 35 dry samples were immersed in 5% natural calcium water for 20 minutes, washed with purified water, 1L alcohol was added to 100g of each sample, and extracted at a temperature of 70-90°C for 2 ...

Embodiment 3

[0069] Embodiment 3 Adding different concentrations of DF feed to induce feeding effect

[0070] In order to investigate the effect of adding different concentrations of DF feed on inducement intake, add DF 0.2g, 0.4g, 0.8g to 1kg of general feed, and when feeding to prawns, the daily feed intake is as follows: Figure 4 shown.

[0071] Feeding the control group without adding DF, the daily feed intake is 0.16±0.02, and feeding the test group with DF 0.2g / kg, the daily feed intake is 0.25±0.02, which is higher than the daily feed intake of the control group. significantly increased (p<0.05). The daily feed intake of the test group fed with DF 0.4g / kg was 0.28±0.03g, which was significantly higher than the daily feed intake of the control group (p<0.01). In the experimental group, there was some increase, but no significant difference was shown. The daily feed intake of the test group fed with DF 0.8g / kg was 0.30±0.03g, which was slightly higher than that of the test group f...

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Abstract

The invention provides a composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof. The composition comprises extractives of the following six cereals: corn, barley, Job's tears, millet, buckwheat and wheat, comprises extractives of the following 25 Chinese herbal medicines: medicated leaven, fructus hordei germinatus, radix et rhizoma rhei, cablin potchouli herb, radix glycyrrhizae, folium perillae, rhizoma atractylodis macrocephalae, rhizoma chuanxiong, radix scutellariae, chinese lizardtail rhiaomeor herb, fructus crataegi, radix angelicae sinensis, radix ginseng rubra, fructus lycii, rhizoma coptidis, radix astragali, fructus schisandrae Chinensis, concha haliotidis, herba artemisiae annuae, poria, cortex magnoliae officinalis, fructus corni, radix aucklandiae, akebia stem and fructus forsythia, comprises the following four algae extractives: laminaria japonica, scagassum, codium fragile and seaweed, and comprises the following three mushroom mycelia: tricholoma gambosum mycelium, lentinus edodes mycelium and cordyceps sinensis mycelium. The composition is applicable as a forage additive. The composition has the advantages of promoting growth of mammalia, poultry, fish, arthropods and most of animals, enhancing resistance and the like, and especially has special efficacy for preventing white spot syndrome of crustacea.

Description

【Technical field】 [0001] The present invention relates to a composition which can enhance immunity or prevent leukoplakia syndrome and its application. 【Background technique】 [0002] According to the degree of infection, 80-100% of the shrimps infected with WSSV died within 3-5 days, and all died within 20 days. White spot syndrome is a fatal disease of arthropods and the biggest problem in shrimp farming (Lightner, 1996). In order to avoid such harm, after 1994, the study of vitiligo syndrome began, the DNA sequence of the causative virus (WSSV) was determined, the PCR inspection method, the histological lesion phenomenon, the infection process and other aspects were studied (Lo et al. al., 1997; Nunam and Lightner, 1997; Supamattaya, et al., 1998; Hossain et al., 2001; Soto et al., 2001). [0003] In order to develop an immunopotentiator against Vibrio and WSSV, various studies have been conducted. For example, glucan extracted from yeast cell wall, peptidoglycan extra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16A61K36/8998A61P31/20A61P37/04
Inventor 赵光善
Owner XIAMEN HONGJIE ENVIRONMENTAL TECH CO LTD
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