A novel enzymatic method for extracting seaweed intermediates

A technology of enzymatic extraction and intermediates, applied in the direction of organic fertilizers, etc., can solve the problems of undiscovered industrialization, and no report on the complete process research of compound enzyme extraction method, etc., to achieve the effect of improved product effect and efficient preparation method

Active Publication Date: 2018-03-16
山东筱萤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After careful investigation by our side, although there are such literature reports in China, they are all at the level of theoretical research, and most of them are phased research results. Some companies have industrialized this technology, so this project has a demonstration role in the same industry and can bring huge economic benefits and industry influence

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  • A novel enzymatic method for extracting seaweed intermediates
  • A novel enzymatic method for extracting seaweed intermediates
  • A novel enzymatic method for extracting seaweed intermediates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 A kind of novel enzymatic method extracts the method for seaweed intermediate, comprises the following steps:

[0039] (1) Weigh 500 parts of dried kelp, wash it with water and soak it to fully absorb water to 1500 parts;

[0040] (2) Weigh 100 parts of water, add 3 parts of phosphoric acid therein, and mix well to obtain a phosphoric acid solution;

[0041] (3) 1500 parts of kelp obtained in step (1) are added to the phosphoric acid solution in step (2) to obtain mixture A;

[0042] (4) Take by weighing 10 parts of compound acid enzyme and dissolve in 500 parts of water, mix uniformly and add in the mixture A obtained in step (3), mix uniformly to obtain mixture B;

[0043] The compound acid enzyme is a mixture of acid xylanase, acid lipase, acid pepsin and acid exonuclease, and its mass ratio is 3:2:2:1;

[0044] (5) Stir the mixture B for 15-90min at a temperature of 40°C;

[0045] (6) Dissolve 65 parts of potassium hydroxide and 15 parts of compound ...

Embodiment 2

[0050] Embodiment 2 A kind of novel enzymatic method extracts the method for seaweed intermediate, comprises the following steps:

[0051] (1) Take 150 parts of dried sea-tangle, wash it with water and soak it to fully absorb water to 1000 parts;

[0052] (2) 200 parts of water are weighed, 3.5 parts of acetic acid are added thereto, and mixed to obtain an acetic acid solution;

[0053] (3) 1000 parts of kelp obtained in step (1) are added to the acetic acid solution of step (2) to obtain mixture A;

[0054] (4) 6 parts of compound acid enzymes are taken and dissolved in 350 parts of water, mixed uniformly and added to the mixture A obtained in step (3), mixed uniformly to obtain mixture B;

[0055] The compound acid enzyme is a mixture of acid chitosanase, acid xylanase, acid lipase, acid pepsin and exonuclease, and its mass ratio is 3:3:2:1:1;

[0056] (5) Stir the mixture B for 20 minutes at a temperature of 40°C;

[0057] (6) Dissolve 10 parts of inorganic basic compoun...

Embodiment 3

[0063] Embodiment 3 A kind of novel enzymatic method extracts the method for seaweed intermediate, comprises the following steps:

[0064] (1) Weigh 400 parts of dried kelp, wash it with water and soak it to fully absorb water to 2000 parts;

[0065] (2) Take by weighing 480 parts of water, add 1.5 parts of sulfuric acid to it, mix well, get sulfuric acid solution;

[0066] (3) 2000 parts of kelp obtained in step (1) are added to the sulfuric acid solution in step (2) to obtain mixture A;

[0067] (4) Take by weighing 13 parts of compound acid enzymes and dissolve in 580 parts of water, mix uniformly and add in the mixture A obtained in step (3), mix uniformly to obtain mixture B;

[0068] Described composite acid enzyme is the mixture of acid carboxylesterase, acid dextrin hydrolase, acid lipase, elastase, chitinase and exonuclease, and its mass ratio is 4:2:3:3: 1:1;

[0069] (5) Stir the mixture B for 30 minutes at a temperature of 55°C;

[0070] (6) Dissolve 70 parts o...

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Abstract

The invention relates to a novel enzymatic method for extracting seaweed intermediates. The steps are as follows: fully wash and soak dried kelp, add it to an acid solution, and stir evenly to obtain a mixture A; dissolve the compound acid enzyme in water and add it to the mixture A for the second step. After one round of stirring and extraction, mixture B is obtained; the inorganic basic compound and compound alkaline enzyme are dissolved in water and added to mixture B for the second round of stirring and extraction to obtain mixture C; the preservative is dissolved in water and added to mixture C , and stir evenly to obtain the seaweed intermediate. The preparation of the seaweed intermediate by the present invention is efficient, rapid, mild, comprehensive, safe and harmless. Compared with the traditional acid-base extraction process, the present invention has an extraction efficiency increased by more than 50%, a seaweed polysaccharide content increased by 50-100%, and alginic acid When the content is between 3.5-4.0%, the effect of the seaweed intermediate product is significantly improved, and all the active ingredients in the seaweed are retained in the product, truly realizing full-effect addition.

Description

technical field [0001] The invention relates to a novel enzymatic method for extracting seaweed intermediates, which belongs to the field of biological resource extraction. Background technique [0002] The ocean area accounts for 70% of the earth's surface. The seaweed that grows in seawater is a large family of marine biological resources. There are 250 genera and more than 1,500 species. Seaweed is rich in various nutrients, including vitamins, polysaccharides, alginic acid, and nectar. Alcohol, betaine, highly unsaturated fatty acids, antibiotics and a variety of natural plant hormones. Seaweed has a simple biological structure, which is conducive to processing and extracting active substances, and has been widely used in medicine, food, industry, agriculture and other fields. Seaweed fertilizer refers to the biological fertilizer produced from seaweed or seaweed extract through fermentation, acid-base process or fertilizer mixing process. The fertilizer has been clear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/10
Inventor 李昆仑赵林许士明张娟孙欣
Owner 山东筱萤生物科技有限公司
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