Drug composition containing glutathione and application of drug composition

A technology of glutathione and composition, applied in the field of medicine and biology, can solve the problems of poor implant restoration, high initial osseointegration, implant failure, etc.

Active Publication Date: 2015-04-29
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The implant restoration of diabetic patients often has poor initial osseointegration and high failure rate. Diabetic patients whose condition is not well controlled are prone to implant failure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 The effect of the pharmaceutical composition comprising GSH on the differentiation and proliferation of human osteoblast-like MG-63 cells under high glucose

[0025] MG-63 cells were placed in culture flasks and cultured with MEM / EBSS medium (containing 10% fetal bovine serum (FBS) and non-essential amino acids (NEAA)). Passage when the cells adhere to the wall and reach about 80-90% confluence in the culture flask. MG-63 cells were inoculated at 2000 cells / well in a 96-well plate and cultured for 24 hours. After 12 hours of serum starvation, the medium was replaced with one of the following: (a) MEM / EBSS (containing NEAA, 5% FBS and 1.0 g / L glucose (physiological sugar concentration, NG)); (b) MEM / EBSS (containing NEAA, 5% FBS and 4.5g / L glucose (high sugar concentration, HG)); adding medium to add GSH and containing GSH at the same time For the culture medium solution of the pharmaceutical composition, in each group, the control group was added with medium...

Embodiment 2

[0036] The preparation of embodiment 2 sustained-release microspheres

[0037] 100mg PLA was dissolved in 1ml of dichloromethane to make the organic phase, then 0.1ml of the drug solution (30mg / ml) was added to the organic phase, ultrasonic (190kW) formed colostrum; colostrum was poured into 8ml concentration In 1% PVA aqueous solution, stir at 1500rpm to form double emulsion, then pour 7ml of PVA aqueous solution, place it on a mechanical stirrer and stir at 600rpm for 6-8 hours, after dichloromethane volatilizes completely, centrifuge to collect the obtained The microspheres are washed with distilled water several times, then collected by centrifugation, placed in a Brookfield hole for suction filtration, and freeze-dried to obtain the sustained-release microspheres.

[0038] Through the above-mentioned method, the present invention has prepared 5 groups of microspheres respectively, and the medicine composition weight ratio of each group is as follows:

[0039] we...

Embodiment 3

[0040] Example 3 Study on the Peri-implant Osseointegration Level of Slow-release Microspheres in Type 2 Diabetic Rats

[0041] Eight-week-old GK rats were fed adaptively for one week, and fed with high-fat and high-sugar diet for 4 weeks, and the rats with blood sugar 18.0mmol / L were eliminated by monitoring the blood sugar of the rats, so as to reduce type 2 DM The mixed bias of rat blood glucose values ​​for this experiment ensures that the experiment will run smoothly.

[0042] Rats were anesthetized with 2% sodium pentobarbital for successful modeling. After anesthesia, the right lower limb of the rat was skin-prepared. A longitudinal incision about 2 cm long was made at the femoral head of the rat’s right lower limb along the direction of the tibia, and the muscle tissue was bluntly separated. Tendons, periosteum, and tibial epiphysis were exposed, and ring dental drills with diameters of 2.2mm and 2.8mm were used to prepare the implant socket under normal saline flushin...

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Abstract

The invention belongs to the field of medicine bioengineering and particularly relates to a drug composition containing glutathione (GSH) and an application of the drug composition. More particularly, the invention provides an application of reduced GSH to preparation of drugs for promoting diabetic implant bone integration, and further provides the drug composition containing GSH. The drug composition can promote diabetic implant bone integration and comprises GSH and other drugs, wherein the other drugs are preferably selected from resveratrol and/or insulin. Furthermore, the invention provides a drug preparation containing the drug composition and a preparation method of the drug preparation; the drug preparation is preferably selected from sustained-release microspheres.

Description

technical field [0001] The invention belongs to the field of medicine and biology, and in particular relates to a pharmaceutical composition containing glutathione and its application. Background technique [0002] Diabetes mellitus is a common endocrine and metabolic disease. It is a systemic chronic metabolic syndrome characterized by elevated blood sugar and urine sugar caused by absolute or relative insulin deficiency and leading to sugar, protein and fat metabolism disorders. At present, the number of diabetic patients in China has reached 50 million, accounting for 1 / 5 of the world's total diabetic population. The prevalence rate ranks second in the world after India, and the number is increasing at a rate of at least 3,000 per day, with an annual increase of more than 1.2 million. Type 2 diabetes mellitus (type 2diabetes mellitus, T2DM) is the main type of diabetes, accounting for more than 90% of all diabetic patients. With economic development and social aging, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/06A61K38/28A61K9/16A61K47/34A61P19/08A61K31/05
Inventor 张静莹
Owner DALIAN UNIV
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