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Method for fermenting aquatic product leftovers by mixed culture bacteria

A technology of aquatic product leftovers and mixed strains, applied in the biological field, can solve the problems of single strains and limitations, and achieve the effects of stable quality, increased added value, and good market development prospects

Inactive Publication Date: 2015-05-06
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many studies on the process optimization of microbial fermentation to produce bacterial fertilizer, but the raw materials are mostly limited to animal manure, rice husk, sludge and straw, etc., the use of a single strain of bacteria, process optimization is also mostly carried out by orthogonal experiments, such as Cao Huiling et al. used fresh cow dung and rice husk powder as fermentation raw materials to conduct an aerobic fermentation experiment of cow dung, and explored the optimal process conditions for the production of bio-organic fertilizers with orthogonal experiments.

Method used

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  • Method for fermenting aquatic product leftovers by mixed culture bacteria
  • Method for fermenting aquatic product leftovers by mixed culture bacteria
  • Method for fermenting aquatic product leftovers by mixed culture bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Prepare culture medium

[0034] Strain Activation Medium: Potato Dextrose Agar (PDA) medium, Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd.

[0035] Seed medium: Potato liquid medium (peel and chop 200g potatoes, boil them to obtain potato juice, add 20g glucose or sucrose, add water to 1000mL).

[0036] Fermentation medium: 10% (w / v) minced low-value aquatic products, 90% distilled water.

[0037] 2 Experimental methods

[0038] 2.1 Culture method

[0039] Plate activation culture: culture at 28°C for 3 days.

[0040] Seed culture: put 50mL of seed medium into a 250mL Erlenmeyer flask, use an inoculation loop to take one ring each of the strain a acidovorans, b pseudomonas, c aspergillus niger, and strain d yeast, and insert them into the sterilized The seed medium was sealed with a parafilm, and cultured on a shaker at 28°C and 120 rpm until the logarithmic phase of the strain was taken out.

[0041] Fermentation culture: put 50 mL of fermentati...

Embodiment 2

[0097] The difference from Example 1 is that the seed culture step: put 50 mL of seed medium in a 250 mL Erlenmeyer flask, use an inoculation loop to take one ring each of strain a and strain b, two rings each of strain c and strain d, and insert The sterilized seed medium was sealed with a parafilm, and cultured on a shaker at 30° C. and 203 rpm until the logarithmic phase of the strain was taken out; the seed medium was potato liquid medium.

[0098] Strain a: Candida tropicalis,

[0099] Strain b: Cryptococcus laurentii,

[0100] Strain c: Bacillus subtilis,

[0101] Strain d: Bacillus cereus.

Embodiment 3

[0103] The difference from Example 1 is that the seed culture step: 50 mL of seed culture medium is loaded into a 250 mL Erlenmeyer flask, one ring of strain a, one ring of bacterial strain b and one ring of bacterial strain c, and two rings of bacterial strain d are taken with an inoculation loop, and the sterile The seed culture medium of the bacteria was sealed with a parafilm, and cultured on a shaker at 30° C. and 203 rpm until the logarithmic phase of the bacteria was taken out; the seed culture medium was potato liquid culture medium.

[0104] Strain a: Candida tropicalis,

[0105] Strain b: Cryptococcus laurentii,

[0106] Strain c: Bacillus subtilis,

[0107] Strain d: Bacillus cereus.

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Abstract

The invention discloses a method for fermenting aquatic product leftovers by mixed culture bacteria. The method is characterized by comprising the following step: fermenting the aquatic product leftovers by a combined bacterial strain, wherein the process parameters are as follows: rotation speed of 203 rpm, an inoculum size of 5% and a temperature of 30 DEG C. Under the condition, the content of nitrogen in free amino acid of the fermented liquor is up to 13.865g / L which is increased by 12.13% in comparison with the content (12.365g / L) before optimization, so that the water-soluble amino acid fertilization standards are met. The method is also suitable for preparing a microbial fertilizer.

Description

technical field [0001] The invention relates to biological technology, in particular to a method for fermenting leftovers of aquatic products by using mixed strains. Background technique [0002] As people's demand for health and food nutrition is getting higher and higher, aquatic products are more and more popular due to their low-fat, high-protein, rich in vitamins and minerals, etc., which brings the development of aquatic product processing industry Rapid development and growth, production increased year by year. However, in the process of processing, a large amount of leftovers will be produced, such as fish heads, fish skins, fish fins, fish tails, fish viscera, fish bones, fish gallbladder, fish floats, shrimp shells, crab shells, shellfish and their residual fish meat, Its weight accounts for about 40% to 55% of the raw fish. Most of these leftovers are discarded, which not only pollutes the environment, but also causes a waste of resources. From the perspective ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05C11/00C05F11/08
CPCC05F1/00C05C11/00C05F11/08
Inventor 罗红宇宋敏刘为童晓倩赵玉谨
Owner ZHEJIANG OCEAN UNIV
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