A kind of extraction method and application of apple fruit cell extract
An extraction method and extract technology, which are applied in pharmaceutical formulations, cosmetic preparations, preparations for skin care, etc., can solve the problems of high maintenance cost, great influence on extraction effect, etc., and achieve shortened extraction time, good filtering effect, The effect of accelerated crushing
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Embodiment 1
[0029] 1. Preparation of apple ingredients
[0030] 1) Choose fresh apples (you can purchase them in the market), rinse them with tap water and then rinse them with deionized water, and then cut the apples into small pieces of 10×10mm.
[0031] 2) Put the small apple pieces in a blast drying box for air drying treatment at 100°C for 24 hours to remove moisture;
[0032] 3) Crush the air-dried apple pieces with a crusher, and then sieve them with a 60-mesh sieve.
[0033] 2. The method of extracting apple extract with the aid of ultrasonic wave and organic solvent under negative pressure, the apple extract is apple polyphenol.
[0034] 1) Weigh a certain amount of apple powder at room temperature, and add 40% acetone solution and 75% ethanol solution as a solvent in a ratio of 1:50:50 for dissolution.
[0035] 2) Adjust the negative pressure to 0.1mPa in the reaction vessel and stir it fully.
[0036] 3) Carry out ultrasonic treatment under the condition of 800W (preferably 500W) ultrasoni...
Embodiment 2
[0066] The specific extraction method steps are basically the same as in Example 1, except that the apple fruit, acetone, and ethanol are mixed at a solid-to-liquid ratio of 1:60:60; ultrasonic treatment is carried out at a negative pressure of 0.5MPa and 800W; The time for each ultrasonic treatment is 2 min.
[0067] 1. Identification of apple polyphenols:
[0068] The apple polyphenols prepared in Example 2 were identified as follows:
[0069] 1) Identification principle: Thin-layer chromatography (adsorption chromatography). Adsorbent thin-layer chromatography is to spread the adsorbent evenly into a thin layer on a smooth surface, and then spot the sample on it and develop it with mobile phase. The sample components are continuously adsorbed by the adsorbent, and dissolved by the mobile phase, desorbed and moved forward. Because the adsorbent has different adsorption capacities for different components, and the mobile phase also has different desorption capacities, the material...
Embodiment 3
[0072] Example 3 Animal experiment
[0073] The specific extraction method steps are basically the same as in Example 1, except that the apple fruit, acetone, and ethanol are mixed at a solid-liquid ratio of 1:30:30; ultrasonic treatment is carried out at a negative pressure of -0.5MPa and 400W ; The time of each ultrasonic treatment is 2min.
[0074] The apple polyphenols prepared in Example 2 were subjected to the following animal experiments:
[0075] Take 50 Kunming mice (each weighing 20g-25g) and randomly divide them into 5 groups: blank control group (group A), model control group (group B), three high, medium and low dose groups of apple polyphenol (Respectively E, D, C groups). The high-dose, medium-dose, and low-dose groups were given apple polyphenols of 150, 100, and 50 mg / kg body weight, and the control group was given the same volume of distilled water. The animals were sacrificed 6 weeks later, and the local SOD and HYP contents were determined by biochemical method...
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