Pesticide for preventing and treating corm rottenness pathogen of saffron crocus as well as preparation and using methods of pesticide

A technology for bulb decay and saffron, which is applied in the directions of botanical equipment and methods, chemicals for biological control, horticultural methods, etc., can solve problems such as decreased control effect, environmental harm, ecological environment damage, etc., and achieves improved control effect, The effect of reducing pollution and high safety

Inactive Publication Date: 2015-05-27
倪春仙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the disease bulb rate of saffron is about 30%. When the storage is improper or the rainy season of the year is long, the disease bulb rate can be as high as 50%. Seriously affect the yield and quality of saffron and the source of bulbs of saffron expansion reproduction
[0004] According to existing literature reports, according to the identification of morphological characteristics of pathogenic bacteria, it is shown that saffron bulb rot is mainly caused by Fusarium oxysporum and anthracnose. The morphological, physiological and biochemical characteristics of genera tend to be similar, and it is difficult to accurately identify the species of pathogenic bacteria only by conventional morphological characteristics
At present, the medicaments used to prevent and treat saffron bulb rot are mainly designed for Fusarium oxysporum and anthracnose, such as carbendazim, chlorothalonil, hymexazol, dexazone and mancozeb, etc. These chemicals Pesticides generally have problems such as limited sterilization, large dosage, and poor control effect, which cannot effectively alleviate the condition of saffron bulb rot; and frequent use of people can easily lead to drug resistance of pathogenic bacteria, resulting in a decline in control effect year by year. The cost of prevention and control is too high, and it also causes great harm to the environment, causing environmental pollution and the increasing destruction of the ecological environment.

Method used

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  • Pesticide for preventing and treating corm rottenness pathogen of saffron crocus as well as preparation and using methods of pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A pesticide for preventing and treating saffron bulb rot, which is prepared from the following raw medicinal materials in parts by weight: 10 parts of Sophora flavescens, 20 parts of Huanan Yunshi, and 20 parts of Western Ivy.

[0023] The preparation method of the pesticide used for saffron bulb rot, the specific preparation steps are as follows: (1) dry Sophora flavescens, South China yunshi and foreign ivy, cut into granules, and pass through a 60-mesh sieve; (2) According to the ratio of material to liquid 1: Add ethanol with a volume fraction of 95% at 6g / ml and mix well; (3) Reflux the mixture twice at 80°C, and combine the filtrate to obtain the extract; (4) Add additives and water to the extract for dilution An aqueous solution with a concentration of 500 ppm is obtained, wherein the additives are antifreeze and dispersant.

Embodiment 2

[0025] A pesticide used for preventing and treating saffron bulb rot is prepared from the following raw medicinal materials in parts by weight: 20 parts of Sophora flavescens, 20 parts of Huanan Yunshi, and 20 parts of Western ivy.

[0026] The preparation method of the pesticide used for saffron bulb rot is characterized in that the specific preparation steps are as follows: (1) Dried Sophora flavescens, South China Yunshi and Ivy, cut into granules, and pass through a 60-mesh sieve; (2) According to the solid-liquid ratio of 1:8g / ml, add ethanol with a volume fraction of 95% and mix well; (3) Reflux the mixture twice at 80°C, and combine the filtrate to obtain the extract; (4) Add additives to the extract Dilute with water to obtain a concentration of 500ppm aqueous solution, wherein the additives are antifreeze, emulsifier and dispersant.

[0027] Wherein, the antifreeze agent is at least one of glycerin, propylene glycol, ethylene glycol, sodium chloride, calcium chloride,...

Embodiment 3

[0031] Embodiment 3: laboratory antibacterial test

[0032] The present embodiment adopts indoor tube-and-dish method to respectively measure the effect of extracting pesticide aqueous solution on saffron bulb rot disease pathogenic bacteria (Serratia marcescens, Serratia rubus, Cypripedium Erwinia) and fungi (Fusarium oxysporum, Trichoderma fungus) , Plexus erythrosum) antibacterial rate.

[0033] (1) Bacteriostasis experiment on pathogenic bacteria of saffron bulb rot

[0034] Prepare potato dextrose agar medium (PDA medium: potato 200g, glucose 20g, agar 18g, water 1000mL, natural pH, 121°C, high-pressure steam sterilization) on the ultra-clean workbench, and then add the concentration before the PDA medium solidifies Extract the pesticide aqueous solution at 500ppm and mix it evenly; after activating the strains of Serratia marcescens, Serratia rubella, and Erwinia cypriphytum respectively, pick an appropriate amount of single colony cells and prepare them with sterile ph...

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Abstract

The invention discloses a pesticide for preventing and treating corm rottenness pathogen of saffron crocus as well as a preparation and a using method of the pesticide. The pesticide is prepared from radix sophorae flavescentis, caesalpinia crista linn and hedera helix. The using method comprises the following steps: drying radix sophorae flavescentis, caesalpinia crista linn and hedera helix in sun, cutting into particles, screening, performing reflux extraction by use of an organic solvent, and diluting to obtain an extracted pesticide aqueous solution; and then, frequently spraying the extracted pesticide aqueous solution to leaves and corm of the saffron crocus at the seedling stage and the flowering stage of the saffron crocus, thereby effectively treating corm rottenness pathogen of saffron crocus. The extracted pesticide disclosed by the invention can prevent and treat the corm rottenness pathogen of saffron crocus with an effective rate of 92.7% and 92.4%, and can effectively relieve the corm rottenness pathogen of saffron crocus. Moreover, the extracted pesticide is extracted from natural plants, and does not generate pesticide residue to the saffron crocus, so that the pollution to the environment can be reduced, the safety is high, and the cost is low.

Description

technical field [0001] The invention relates to the field of plant disease prevention and control, in particular to a pesticide for preventing and treating saffron bulb rot and its preparation and use methods. Background technique [0002] Saffron is native to southern Europe and Iran, and is also cultivated in Shanghai, Zhejiang, Jiangsu, Shandong, Beijing and other places in my country. The stigma of saffron is its main medicinal part, which has the effects of promoting blood circulation and dispelling fatigue, cooling blood and detoxification, relieving stagnation and calming nerves, can inhibit hepatitis virus, improve immunity, and has anticancer activity, known as "plant gold". In addition, saffron can also be used as a precious flavoring agent, natural color, spice and dye. [0003] Due to changes in farmland ecological pathways and increased resistance to plant diseases, saffron bulb rot has become the most serious disease in saffron production in recent years. From...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/20A01P3/00A01P1/00A01G7/06A01G13/00
Inventor 倪春仙
Owner 倪春仙
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