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Preparation method of pepsin and trypsin treated Zhibai Dihuang composite micro-ecological oral liquid

A technology of compound microecology and trypsin, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, drug combinations, etc., to achieve the effect of reducing side effects and hindering digestion

Inactive Publication Date: 2015-06-03
白杨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned patent can extract and concentrate the medicinal ingredients in Zhibai Dihuang Pills to produce concentrated Zhibai Dihuang Pills, the side effects of Zhibai Dihuang Pills that hinder digestion have not been weakened

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] The preparation steps of the tomato extract are as follows:

[0042] Wash and weigh fresh tomatoes, chop them up, add an equal amount of distilled water, heat in a water bath at 100°C, stir for 90 minutes, then filter with gauze to adjust the pH to 7.0;

[0043] The preparation steps of the liver extract are as follows:

[0044] Weigh 1000g of fresh pork liver, cut into small pieces or mince, add distilled water to 2000mL, mix well, put in the refrigerator overnight, boil for 15min the next day, filter with gauze and squeeze to collect the filtrate, add water to make up to 2000mL.

[0045] The MRS liquid culture medium in the embodiment has every liter of components as follows:

[0046] Peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ] 2.0g, glucose (C 6 h 12 o 6 ·H 2 (O) 20.0g, Tween 801.0mL, sodium acetate (CH 3 COONa·3H 2 O) 5.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0g, magnesium...

Embodiment 1

[0048] A preparation method of Zhibai Dihuang compound microecological oral liquid treated with stomach and trypsin, the steps are as follows:

[0049] (1) Pulverize the Bawei raw material medicine in Zhibai Dihuang Pills to obtain a raw material powder, then add 200 grams of water and refined honey for every 100 grams of raw material powder, and mix evenly to obtain a raw material slurry;

[0050] (2) Add hydrochloric acid to the raw material slurry prepared in step (1) to adjust the pH value to 1.5, add 20 activity units of pepsin per 100 grams of Zhibai Dihuang Wan raw material, and treat for 3 hours at a temperature of 36 ° C, add sodium carbonate Adjust the pH value to 8 to prepare pepsin-treated slurry;

[0051] (3) Add 50 activity units of trypsin per 100 ml to the pepsin-treated slurry prepared in step (2), treat at a temperature of 37° C. for 2 hours, and adjust the pH value to 5.5 with hydrochloric acid to obtain stomach, Trypsinized slurry;

[0052] (4) In the slu...

Embodiment 2

[0056] A preparation method of Zhibai Dihuang compound microecological oral liquid treated with stomach and trypsin, the steps are as follows:

[0057] (1) Pulverize the Bawei raw material in Zhibai Dihuang Pills to obtain a raw material powder, then add 600 grams of water and refined honey for every 100 grams of raw material powder, and mix evenly to obtain a raw material slurry;

[0058] (2) Add acid to the raw material slurry prepared in step (1) to adjust the pH value to 2.2, add 60 activity units of pepsin per 100 grams of Zhibai Dihuang Wan raw material, treat at 38°C for 2 hours, and add sodium carbonate Adjust the pH value to 7.8 to obtain pepsin-treated slurry;

[0059] (3) Add 20 activity units of trypsin per 100 ml to the pepsin-treated slurry prepared in step (2), treat at a temperature of 37° C. for 3 hours, and adjust the pH value to 6 with hydrochloric acid to obtain stomach, Trypsinized slurry;

[0060] (4) In the stomach and trypsin-treated slurry prepared i...

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Abstract

The invention relates to a preparation method of pepsin and trypsin treated Zhibai Dihuang composite micro-ecological oral liquid. The preparation method comprises the following steps: (1) crushing Zhibai Dihuang pill raw materials, adding water, and mixing uniformly to prepare raw material serous fluid; (2) adding acid into the raw material serous fluid to adjust the pH value, and adding pepsin for treatment, thereby preparing pepsin treated serous fluid; (3) adding trypsin into the pepsin treated serous fluid for treatment to prepare the pepsin and trypsin treated serous fluid; and (4) inoculating bifidobacteria and lactobacillus plantarum to obtain the pepsin treated Zhibai Dihuang composite micro-ecological oral liquid. The pepsin and trypsin treated Zhibai Dihuang composite micro-ecological oral liquid prepared by the preparation method provided by the invention is relatively small in side effect, and is palatable in sweet and sour tastes; and by virtue of practical application, after being taken for a long time, the pepsin and trypsin treated Zhibai Dihuang composite micro-ecological oral liquid not only can achieve a treatment effect of Zhibai Dihuang pills but also can greatly reduce the side effects causing stomach cold and digestion obstruction.

Description

technical field [0001] The invention relates to a preparation method of Zhibai Dihuang composite micro-ecological oral liquid treated with stomach and trypsin, in particular to a method for processing the classic Zhibai Dihuang prescription by using biotechnology, which belongs to the technical field of biomedicine. Background technique [0002] Zhibai Dihuang Wan is a commonly used Chinese patent medicine. It is made from Liuwei Dihuang Wan (rehmannia glutinosa, cornus, yam, Alisma Moutan bark and Poria cocos), which is a classic representative prescription for nourishing yin, and Anemarrhena Phellodendron. effect. Traditionally used for symptoms such as yin deficiency and hyperactivity of fire, hot flashes and night sweats, dry mouth and sore throat, tinnitus and nocturnal emission, and short red urine. In recent years, it has been used flexibly after TCM syndrome differentiation, and it also has good curative effect on several diseases such as chronic pharyngitis and acu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61K9/08A61P1/14A61P11/04A61P13/00A61P31/00A61K35/644A61K35/745
Inventor 白杨
Owner 白杨