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Application of Fuzhuan tea in regulation of animal intestinal flora

A technology of intestinal flora and fucha, which can be applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, drug combinations, etc. Effects without toxic side effects

Inactive Publication Date: 2015-07-01
BGI SHENZHEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current research on regulating the gut microbiota in animals still needs to be in-depth

Method used

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  • Application of Fuzhuan tea in regulation of animal intestinal flora
  • Application of Fuzhuan tea in regulation of animal intestinal flora
  • Application of Fuzhuan tea in regulation of animal intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of fu tea

[0034] 1. Preparation of spore suspension of S. coronis

[0035] Use a sterile inoculation needle to pick out the yellow obturator shells on Fuzhuan brick tea cubes and inoculate them on Chapei's medium, and cultivate them at 28°C. When cultured for 72 hours, pick a small amount of yellow closed cysts and separate them by streaking on a plate. After 2 to 3 streaks on a plate, the bacteria has been preliminarily purified. When the initially purified bacterial colony is in the vigorous growth period, use the inoculation loop to scrape the yellow occiput shell into a sterile vial filled with 10ml of sterile water, shake for 20min to make a uniform spore suspension and increase it by 10 times diluted to make 10 -3 ~10 -5 Diluted spore suspension.

[0036]Use a sterile pipette to draw 0.1ml of the spore suspension of each dilution and spread it on the blank Chapeau medium, and then culture it at 28°C. Select a plate with only 1 ...

Embodiment 2

[0044] Example 2: Function experiment of Fu tea regulating human intestinal flora

[0045] According to the Ministry of Health "Technical Specifications for Testing and Evaluation of Health Drinks (2003)", the method for evaluating the function of intestinal flora in human body is tested and evaluated for the Fucha prepared in Example 1, as follows:

[0046] 1. Drinking experiment group

[0047] Table 1 Grouping table of drinking experiment

[0048]

[0049] 2. Human body drinking test method

[0050] The subjects were randomly divided into two groups, 20 people in each group. Before the subjects tried to drink, the feces of the subjects were collected aseptically, and the intestinal flora was tested by 16S rDNA sequencing as a reference for the background level.

[0051] According to the grouping situation in Table 1, group 1 took the control group and group 2 took the experimental group. After 4 weeks, the feces of the subjects were collected, and 16SrDNA sequencing was...

Embodiment 3

[0060] Example 3: Fu tea lowering blood fat human body food trial experiment

[0061] According to the Ministry of Health's "Health Food Inspection and Evaluation Technical Specifications (2003)", the method for evaluating the blood fat-lowering function human body test experiment evaluation method is used to evaluate the Fu tea prepared in Example 1, as follows:

[0062] 1. Human body test food and test method:

[0063] 20 cases of type II hyperlipidemia were selected on a voluntary basis, including 16 males and 4 females, aged from 43 to 75 years old, without serious complications such as heart, liver and kidney, and a comparative design was adopted before, during, and after eating.

[0064] The subjects were randomly divided into 2 groups, 10 people in each group. The types of therapeutic drugs, diet control and activities used by the subjects were unchanged. The experimental group took the Fu tea of ​​the present invention, and the control group took normal saline. The dos...

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Abstract

The invention discloses application of Fuzhuan tea in regulation of animal intestinal flora. The Fuzhuan tea can be used for obviously increasing the number of such probiotics in animal intestines as lactobacillus acetotoleran, butyrivibrio, Luosibairui Escherichia, Roseburia Stanton and Savage and the like and significantly regulating the animal intestinal flora, so as to achieve an effect of reducing blood fat through the functions of the intestinal flora.

Description

technical field [0001] The invention relates to a new application of Fucha, in particular to the application of Fucha in regulating intestinal flora of animals. Background technique [0002] There are approximately 104 normal flora inhabiting the human gastrointestinal tract, the total amount of which is similar to that of the liver. Gut microbes contain a wide variety of enzyme systems, which can participate in a series of physiological processes such as host energy, material and genetic information transfer. Many studies have proved that intestinal microorganisms can form a biological barrier in the intestinal tract, and inhibit the excessive growth of opportunistic pathogenic bacteria and foreign pathogenic bacteria through space occupation effect, nutrient competition and various metabolites and bacteriocins secreted invasion. More and more evidence shows that the intestinal flora with structural imbalance plays a very important role in the occurrence and development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/10A61K36/82A61P1/00A61P3/06
Inventor 朱倩张海峰丁淑娟朱江阳朱端清张珊珊过敏张亮波刘天翔张耕耘金桃
Owner BGI SHENZHEN CO LTD
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