Intensive octopus variabilis overwintering breeding method
A cultivation method and technology of octopus, which are applied in the fields of climate change adaptation, fish farming, application, etc., can solve the problems of difficulty in obtaining economic benefits, high individual mortality, and erosion of the rear end of the carcass, and improve the survival rate and health. , the effect of high breeding density, reducing dependence and destruction
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Embodiment 1
[0015] Embodiment 1: the screening of flora
[0016] The inventor collects water samples from long octopus overwintering cultivation ponds with low incidence, packs them into sterile sampling bags and takes them back to the laboratory. Put the water sample into the cultivation bucket for continuous culture, and mix it with the pre-prepared beef extract peptone medium added with urea; the formula of the beef extract peptone medium added with urea is as follows: beef extract 0.5g, peptone 1g, sodium chloride 0.5g, urea 0.6g, agar 2g, distilled water 100mL, pH 7.0~7.2. Microscopically check the number of microorganisms every 5 days. When the number of viable bacteria is maintained above 100 / mL, select and acclimatize the dominant bacteria. The first-generation acclimatization system is supplemented with 1 / 4 of the long octopus culture water, 3 / 4 of the beef extract peptone culture solution, and inoculated with flora, and the inoculum amount is 20% (v / v). Shake at 12°C at 180rpm...
Embodiment 2
[0021] (1) Preparation of long octopus nests: In July 2011, 220m of PVC pipes with a diameter of 10cm were purchased at the building material market in Rongcheng City, cut into small sections of 60cm, and polished at both ends, a total of 360 pieces. The PVC pipe section and rice husk-based activated carbon fiber were disinfected with potassium permanganate and rinsed before use.
[0022] (2) The octopus nest of microorganism immobilization is made by the porous rigid polyurethane foam of microorganism immobilization and PVC pipe suit; Its preparation method is as follows:
[0023] Add rice husk-based activated carbon fiber to the base liquid and stir at high speed to make a working liquid, then add PVC pipes to the working liquid, form and age to make octopus nests; add distilled water to the microbial flora to make a bacterial liquid, and then add polyvinyl alcohol Make a cold bacterial suspension, and evenly spray it into the above-mentioned octopus nests, and cross-link wi...
Embodiment 3
[0028] (1) Intensive cultivation facility for long octopus: overwintering cultivation pond for long octopus 5m×3.5m×1.5m (length×width×height), and a total of 360 octopus nests were put in. The 360 octopus nests are divided into 15 piles, each pile is composed of 24 octopus nests, and the size of each pile is 0.6m×0.6m×0.72m (length×width×height). Each pile consists of 6 layers, and the two layers are vertically crossed. The number of each layer of octopus nests is 4, and the distance between two pairs is 4cm. The 15 piles are arranged in 3 rows, the 1st and 3rd rows are close to the 5m wall of the cultivation pool, and the angle between each octopus nest and the wall is 45°C, which can avoid strong stimulation of the long octopus individuals on one side by the water flow. The distance between the second column and the first and third columns is 50cm, which is convenient for managers to observe and clean up residual bait.
[0029] (2) Intensive cultivation and management o...
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