Intensive octopus variabilis overwintering breeding method
A cultivation method and technology of octopus, which are applied in the fields of climate change adaptation, fish farming, application, etc., can solve the problems of difficulty in obtaining economic benefits, high individual mortality, and erosion of the rear end of the carcass, and improve the survival rate and health. , the effect of high breeding density, reducing dependence and destruction
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[0015] Example 1: Screening of flora
[0016] The inventor collected water samples from the octopus overwintering cultivation tank with low incidence, packed them into sterile sampling bags and brought them back to the laboratory. Put the water sample into the cultivation tank for continuous cultivation, and mix it with the pre-prepared beef extract peptone medium supplemented with urea; the formula of the beef extract peptone medium supplemented with urea is as follows: beef extract 0.5g, peptone 1g, sodium chloride 0.5g, 0.6g urea, 2g agar, 100mL distilled water, pH 7.0~7.2. The number of microorganisms was microscopically checked every 5 days. When the number of viable bacteria was maintained above 100 / mL, the selection and domestication of dominant bacteria were carried out. The first-generation domestication system added 1 / 4 of the octopus aquaculture water, supplemented with 3 / 4 of the beef extract peptone culture medium, and inoculated the flora, and the inoculation am...
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[0020] Example 2
[0021] (1) Preparation of octopus nests: In July 2011, 220m PVC pipes with a diameter of 10cm were purchased from the Rongcheng building materials market, cut into small sections of 60cm, and polished at both ends, totaling 360 pieces. The PVC pipe section and the rice husk-based activated carbon fiber are sterilized with potassium permanganate and rinsed before use.
[0022] (2) Microorganism-immobilized octopus nest is made of microbe-immobilized porous rigid polyurethane foam and PVC pipe suit; its preparation method is as follows:
[0023] The rice husk-based activated carbon fiber is added to the base solution and stirred at high speed to make the working solution, then the PVC pipe is added to the working solution, and it is formed and aged to make octopus nests; the microbial flora is added with distilled water to make the bacteria solution, and then polyvinyl alcohol is added. A cold bacterial suspension was prepared, and sprayed evenly into the abo...
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[0027] Example 3:
[0028] (1) Intensive cultivation facilities for octopus: the octopus overwintering cultivation pool is 5m×3.5m×1.5m (length×width×height), and the total number of octopus nests is 360. The 360 octopus nests were divided into 15 piles, each pile consisted of 24 octopus nests, and the size of each pile was 0.6m×0.6m×0.72m (length×width×height). Each stack consists of 6 layers, and the two layers are arranged in a vertical cross. The number of octopus nests in each layer is 4, and the spacing between them is 4cm. The 15 piles were arranged in 3 rows, the first and third rows were close to the 5m wall of the cultivation pool, and the angle between each octopus nest and the pool wall was 45°C, so as to avoid the strong stimulation of the long octopus on one side by the water flow. The distance between the second column and the first and third columns is 50cm, which is convenient for managers to observe and clean up residual bait.
[0029] (2) Intensive cult...
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