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Preparation method of high-biocompatibility dual-target modified Fe3O4 nano material

A technology of biocompatibility and nanomaterials, applied in the field of preparation of functional Fe3O4 nanomaterials, can solve problems such as cumbersome synthesis steps and poor target recognition ability, achieve high relaxation rate, good biocompatibility, and facilitate The effect of mass production

Inactive Publication Date: 2015-07-22
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of current tumor targeting Fe 3 o 4 Due to the potential biological toxicity, poor target recognition ability and relatively cumbersome synthesis steps of nanomaterials, the development of Fe-based 3 o 4 Novel targeting molecular probes for nanomaterials providing a biocompatible dual-target modified Fe 3 o 4 Preparation methods of nanomaterials

Method used

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  • Preparation method of high-biocompatibility dual-target modified Fe3O4 nano material
  • Preparation method of high-biocompatibility dual-target modified Fe3O4 nano material
  • Preparation method of high-biocompatibility dual-target modified Fe3O4 nano material

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Effect test

Embodiment 1

[0022] A dual-target modified Fe with good biocompatibility 3 o 4 The preparation method of nanometer material, the steps are as follows:

[0023] 1) In a 100mL three-neck round bottom flask, 0.5g FeCl 3 ·6H 2 O and 0.184 g FeCl 2 4H 2 O dissolved in 20mLH 2 O, dissolved by mechanical stirring, and protected by nitrogen gas. After 15min, adding 7.5mL mass concentration to the above solution was 27%NH 4 OH, then add 5mL hyaluronic acid (molecular weight 6k Da, 40mg / mL), and continue the reaction at room temperature for 30min.

[0024] 2) the obtained hyaluronic acid modified Fe 3 o 4 The nanomaterials were washed four times with high-purity water using an external magnetic field to remove reaction impurities, and then the materials were dissolved in high-purity water and stored at 4°C.

[0025] 3) Take 5ml of the hyaluronic acid-modified Fe obtained in step 2) 3 o 4 For nanomaterials, use a magnet to suck out the nanomaterials, discard the supernatant, redissolve th...

Embodiment 2

[0029] A dual-target modified Fe with good biocompatibility 3 o 4 The preparation method of nanometer material, the steps are as follows:

[0030] 1) In a 100mL three-neck round bottom flask, 0.25g FeCl 3 ·6H 2 O and 0.092 g FeCl 2 4H 2 O dissolved in 20mLH 2 O, dissolved by mechanical stirring, and protected by nitrogen gas. After 15min, adding 5mL mass concentration to the above solution was 27%NH 4 OH, then add 7.5mL hyaluronic acid (molecular weight 3k Da, 40mg / mL), and continue the reaction at room temperature for 30min.

[0031] 2) the obtained hyaluronic acid modified Fe 3 o 4 The nanomaterials were washed four times with high-purity water using an external magnetic field to remove reaction impurities, and then the materials were dissolved in high-purity water and stored at 4°C.

[0032] 3) Take 5ml of the hyaluronic acid-modified Fe obtained in step 2) 3 o 4 For nanomaterials, use a magnet to suck out the nanomaterials, discard the supernatant, redissolve t...

Embodiment 3

[0036] A dual-target modified Fe with good biocompatibility 3 o 4 The preparation method of nanometer material, the steps are as follows:

[0037] 1) In a 100mL three-neck round bottom flask, 1g FeCl 3 ·6H 2 O and 0.368 g FeCl 2 4H 2 O dissolved in 20mLH 2 O, dissolved by mechanical stirring, and protected by nitrogen gas. After 15min, add 10mL mass concentration to the above solution and be 27%NH 4 OH, then add 10mL hyaluronic acid (molecular weight 4k Da, 40mg / mL), and continue the reaction at room temperature for 30min.

[0038] 2) the obtained hyaluronic acid modified Fe 3 o 4 The nanomaterials were washed four times with high-purity water using an external magnetic field to remove reaction impurities, and then the materials were dissolved in high-purity water and stored at 4°C.

[0039] 3) Take 5ml of the hyaluronic acid-modified Fe obtained in step 2) 3 o 4 For nanomaterials, use a magnet to suck out the nanomaterials, discard the supernatant, redissolve the ...

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Abstract

The invention relates to a preparation method of a high-biocompatibility dual-target modified Fe3O4 nano material, which comprises the following steps: dissolving ferric chloride and ferrous chloride ( the mole ratio of Fe<3+> to Fe<2+> is 2:1) in water by stirring, introducing an inert gas protective atmosphere, sequentially adding ammonia water and hyaluronic acid into the solution, reacting at room temperature for some time, carrying out cleaning purification on the obtained Fe3O4 nano material, dissolving the Fe3O4 nano material in a PBS (phosphate buffer solution), sequentially adding EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) and NHS, stirring to react, and adding a targeted protein molecule to continue the reaction; and after the reaction finishes, carrying out cleaning purification on the obtained product again, dissolving the product in the PBS, and preserving at 4 DEG C. The method has the advantages of simple preparation process, environment-friendly raw materials and mild conditions. The obtained product has the advantages of favorable water solubility, favorable nuclear magnetism, high biocompatibility and high tumor targeted recognition capability, is convenient for large-scale production, and has great development potential and application value in the field of biological imaging.

Description

technical field [0001] The present invention relates to functional Fe 3 o 4 The field of preparation of nanomaterials, especially Fe 3 o 4 Preparation methods of nanomaterials. Background technique [0002] Fe 3 o 4 Nanomaterials have been widely used in the fields of magnetic resonance imaging, protein separation, drug delivery, magnetothermal therapy, and photothermal therapy due to their rich physical and chemical properties, excellent magnetic properties, and easy surface modification. Due to Fe 3 o 4 Nanomaterials can significantly shorten the T of surrounding water molecules 2 relaxation time, and has a high relaxation rate, so the nanomaterial has become the most widely used T 2 Negative contrast agents for weighted magnetic resonance imaging play a vital role in basic research and clinical disease diagnosis. [0003] Currently Fe 3 o 4 The preparation methods of nanomaterials include coprecipitation method, hydrothermal method and thermal decomposition me...

Claims

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Application Information

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IPC IPC(8): A61K49/14
Inventor 于春水潘金彬孙少凯王雅琼
Owner TIANJIN MEDICAL UNIV
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