Device and method for producing butyl alcohol through continuous fermentation of lignocellulose
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A technology for lignocellulose and butanol, which is applied in the directions of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of complicated operation and increase of data detection points, and achieves simple fermentation equipment and improved utilization. The effect of high efficiency and production intensity
Active Publication Date: 2015-09-30
CHINA PETROLEUM & CHEM CORP +1
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It uses fermentation tanks connected in series for continuous fermentation to produce alcohol. However, multiple fermentation tanks are connected in series, and each fermentation tank needs to be equipped with a separate stirring device, and the fermentation tanks need to be connected by pipelines. There are also problems with more data detection points and complicated operations.
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Embodiment 1
[0031] Clostridium acetobutylicum ( Clostridium acetobutylicum ) ATCC 824, purchased from the American Type Culture Collection. The fermentation medium uses the above-mentioned lignocellulose hydrolyzate as the carbon source, the total sugar concentration is 80g / L, and the following nutrients are added in g / L: Yeast 1.0, CH 3 COONH 4 2.2, KH 2 PO 4 0.5,K 2 HPO 4 0.5, MnSO 4 0.01, NaCl 0.01, MgSO 4 ?7H 2 O 0.2, FeSO 4 0.01, p-aminobenzoic acid 0.001, vitamin B 1 0.001, biotin 0.01×10 -3 . The fermented seed solution was obtained by inoculating Clostridium acetobutylicum ATCC 824 into the RCM seed medium and culturing at 37 °C for 20 h under anaerobic environment.
[0032] Using the continuous fermentation operation mode described in the present invention, add the above-mentioned fermentation medium into the first-stage fermenter, the addition amount is half of the volume of the fermenter, and insert the butanol fermenting bacteria seed liquid for cultivation,...
Embodiment 2
[0037] The treatment process and operating conditions are the same as in Example 1, except that Clostridium beijerinckii CM20 is used, the fermentation medium uses the above-mentioned lignocellulose hydrolyzate as a carbon source, the concentration of reducing sugar is 60g / L, and the following nutrients are added, Calculated in g / L: Yeast 1.0, CH 3 COONH 4 2.2, KH 2 PO 4 0.5,K 2 HPO 4 0.5, MnSO 4 0.01, NaCl 0.01, MgSO 4 ?7H 2 O 0.2, FeSO 4 0.01, p-aminobenzoic acid 0.001, vitamin B 1 0.001, biotin 0.01×10 -3 . The fermented seed solution was obtained by inoculating Clostridium beijerinckii CM20 into the RCM seed medium and culturing at 37 °C for 20 h in an anaerobic environment. Control the dilution rate of the primary fermenter at 0.03h -1 , taking the first overflow of the fermentation broth from the secondary fermenter as the starting point of continuous fermentation, the results are shown in Table 2 below.
[0038] The fermentation result of table 2 embo...
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Abstract
The invention discloses a device for producing butyl alcohol through continuous fermentation of lignocellulose. A primary fermentation tank is a stirring kettle type fermentation tank, and a secondary fermentation tank is a cuboid tubular fermentation tank; vertical partition plates are arranged in the tubular fermentation tank to divide the tubular fermentation tank into multiple same reaction units; the lower end of the partition plate is fixedly connected with the bottom of the fermentation tank, and a water channel is reserved at the upper end of the partition plate; each reaction unit is equipped with an inverted T-shaped barrier plate; the T-shaped barrier plate is fixedly connected with the front and back ends of the reaction unit; and the top end of the reaction unit is fixedly connected with the top of the fermentation tank. According to a method for producing butyl alcohol through continuous fermentation using the device, the secondary fermentation tank is an empty tank before fermentation; and the fermentation liquid flows from the primary fermentation tank and through the top of the first reaction unit of the secondary fermentation tank by use of liquid level difference, overflows to each reaction unit step by step and finally flows out of the last reaction unit. By adopting the device and method disclosed by the invention, continuous production of butyl alcohol can be realized, the operation process is simple, the equipment utilization rate is high, and relatively high total solvent yield and solvent productivity can be obtained at the same time.
Description
technical field [0001] The invention belongs to the technical field of biomass energy, and relates to a device and method for producing butanol by fermenting lignocellulose, in particular to a device and method for continuously fermenting and producing bio-butanol by using lignocellulosic biomass enzymatic hydrolysis liquid. Background technique [0002] Butanol is an important chemical raw material widely used in the manufacture of various plastic and rubber products and in the synthesis of chemicals such as butyraldehyde, butyric acid, butylamine and butyl lactate. In recent years, with the development of downstream industries, the market demand has risen sharply. In 2013, the import volume of butanol in my country was about 400,000 tons; butanol is also a new type of biofuel with great potential after fuel ethanol. , The octane number is equivalent to that of gasoline, the vapor pressure is low, and it is miscible with gasoline in any ratio. It has high safety performance ...
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