Preparation method of porous bioceramic microsphere bone scaffold grafted with bone cells on surface

Abstract

The invention discloses a preparation method of a porous bioceramic microsphere artificial bone scaffold grafted with bone cells on a surface, belonging to the technical field of biological tissue engineering. The preparation method comprises the following steps: firstly, establishing a CAD model according to the individual characteristics of a patient, importing the model into a 3D printer, and spraying a biological adhesive by the 3D printer to bond porous bioceramic microspheres, thereby realizing the preparation of an artificial bone scaffold substrate; next, performing multiple subculture on osteoblasts taken from the patient to reach the quantity demanded, and then mixing the osteoblasts with a DMEM cell culture medium, a bone growth factor and a biocompatible hydrogel into a specific mixture; and finally, printing the mixture on the prepared porous bioceramic artificial bone scaffold by use of a self-made biological forming machine, thereby obtaining the porous bioceramic microsphere artificial bone scaffold grafted with bone cells on the surface. The method is capable of realizing the customizability of the bone scaffold, the bone scaffold has better biocompatibility, and after the bone scaffold is transplanted into the body of the patient, the wound is easier to heal and the pain of the patient can be relieved.

Description

Technical field [0001] The invention relates to a method for preparing a porous bioceramic microsphere artificial bone scaffold with bone cells inoculated on the surface, and belongs to the technical field of biological tissue engineering. Background technique [0002] Bone tissue is the hardest connective tissue in the human body and plays an irreplaceable role in maintaining body functions. As the stent of the human body, bones are responsible for the functions of support, maintenance, weight bearing, hematopoiesis, calcium storage and metabolism. The treatment of bone defects has been a major problem that plagued humans for a long time. It has been clinically proven that autologous bone transplantation is the best way to treat bone defects, but at the expense of healthy tissue. Allogeneic bone transplantation has superior histological characteristics, but it has immune rejection, is susceptible to virus infection, and the cost of sample preparation, processing and storage is ...

AI Technical Summary

Problems solved by technology

However, this method still has the following problems: the organic binder for the preparation of the scaffold needs to be burned at high temperature, which prolongs the preparation time of the scaffold, increases the preparation cost, and increases the probability of contamination of the bioceramic particles. Precisely control the porosity of the skeleton

Its main disadvantage is that it is difficult to control the spraying amount of the binder, and it cannot achieve precise and controllable mechanical properties; secondly, this method is more focused on achieving controllable mechanical properties, and also does not focus on the compatibility of the support. question

[0007] Through the analysis of the above patents, we can see that the current preparation methods of artificial bone scaffolds are still relatively traditional, and only focus on a certain aspect of research, which cannot greatly meet the requirements of the human body for bone scaffolds. There are certain deficiencies, especially in the compatibility of stents