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Inflammatory factor adsorbing agent for blood perfusion and preparation method

A technology of inflammatory factors and adsorbents, which is applied in the field of inflammatory factor adsorbents and preparations, can solve the problems of general removal of inflammatory factors, poor blood compatibility, and human hazards of coating materials, so as to improve hydrophilicity , Improve skin symptoms, good blood compatibility

Active Publication Date: 2015-10-07
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are reports on the clinical application of continuous blood purification in China, but its effect on removing inflammatory factors is mediocre.
[0007] Most of the existing adsorbents use polystyrene divinylbenzene, which has poor blood compatibility, and the adsorbent needs to be coated. The commonly used coating materials include collodion, polyvinyl alcohol and other hydrophilic materials.
However, there is a potential harm to the human body caused by the shedding of the coating material

Method used

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  • Inflammatory factor adsorbing agent for blood perfusion and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] polymethyl methacrylate adsorbent

[0036] 1-1 Amination reaction

[0037] Take polymethyl methacrylate (PMMA) resin microspheres (pore diameter 5-50nm, specific surface area greater than 500m 2 / g) Add 10mL of dichloroethane to 10g and soak overnight, then add 10mL of 50% hexamethylenediamine in dimethylformamide solution, stir and react at 130°C for 10 hours at 200rpm. After the reaction was completed, the resin microspheres were washed with alcohol and purified water respectively.

[0038] 1-2 Condensation reaction with cysteine

[0039] Take the resin microspheres obtained in 1-1, add 20mL of 1% cysteine ​​solution, adjust the pH to 4.8, add 0.8g NHS (N-hydroxysuccinimide), and then slowly add 0.4g 1-(3 -Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), shaking and reacting at 25°C for 4 hours. After the reaction was completed, the resin was washed with alcohol and purified water respectively.

[0040] 1-3 Acetylation reaction

[0041] Take the r...

Embodiment 2

[0043] Polystyrene divinylbenzene resin adsorbent

[0044] 2-1 Epoxidation pretreatment

[0045] Take 10g of polystyrene divinylbenzene resin microspheres (pore diameter 5-50nm, specific surface area not less than 500m 2 / g, cross-linking agent divinylbenzene purity 80%) add 40mL dichloroethane to soak overnight, add 20mL 1% m-chloroperoxybenzoic acid acetone solution drop by drop, place in an ice-water bath (temperature controlled at 2°C ) stirring reaction for 24 hours at a speed of 200 rpm. After the reaction was completed, the resin microspheres were washed with alcohol and purified water respectively.

[0046] 2-2 Amination reaction

[0047] Take 2-1 resin, add 30mL of 2% hexamethylenediamine aqueous solution, shake and react at 60°C for 24 hours. After the reaction was completed, the resin microspheres were washed with alcohol and purified water respectively.

[0048] 2-3 Condensation reaction with cysteine

[0049]Take the resin obtained in 2-2, add 20mL of 1.2%...

Embodiment 3

[0053] Cellulose microsphere carrier adsorbent

[0054] Take 10mL of cellulose microspheres, add 20mL of 5% epichlorohydrin, and shake at 40°C for 4 hours. After washing, add 30mL of 2% ethylenediamine aqueous solution, and shake and react at 60°C for 24 hours. After the reaction was completed, the cellulose microspheres were washed with alcohol and purified water respectively. Next, add 20mL of 1.2% cysteine ​​solution, adjust the pH to 4.8, add 0.5g NHS (N-hydroxysuccinimide), and then slowly add 0.4g 1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide hydrochloride (EDC), shaking reaction at 25°C for 5 hours. After the reaction was completed, the cellulose microspheres were washed with alcohol and purified water respectively. After drying, 20 mL of 6% acetyl chloride and triethylamine in dimethylformamide were added dropwise and reacted with shaking at 30°C for 2 hours. After the reaction was completed, the cellulose microspheres were washed with alcohol and purified water...

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Abstract

The invention discloses an inflammatory factor adsorbing agent for blood perfusion and a preparation method. A framework of the inflammatory factor adsorbing agent is a carrier; and the surface of the carrier is in coupling with acetylated cysteine through an amidogen connecting arm. The carrier and the aglucon of the adsorbing agent can adsorb and remove inflammatory factors. Compared with a common adsorbing agent adsorbing the inflammatory factors by a carrier only, the inflammatory factor adsorbing agent has high removing ability. By the adsorbing agent, the inflammatory factors can be adsorbed from blood selectively, and the removing rate of the inflammatory factor adsorbing agent on IL-6 and TNF-alpha is much greater than that of a common poly (styrene-co-divinyl benzene) adsorbing agent. The adsorbing agent has high blood compatibility and can be used for whole blood perfusion.

Description

technical field [0001] The invention relates to polymer biomedical materials, in particular to an inflammatory factor adsorbent for hemoperfusion and its preparation technology. Background technique [0002] Inflammatory cytokines are a general term for a group of polypeptide cell regulatory substances, including interleukins, interferons, growth factors, cell stimulating factors, tumor necrosis factors, etc. Many disease states manifest in the presence of high concentrations of inflammatory factors in the bloodstream. Inflammatory cytokines help stimulate and regulate the immune system to help the body fight infection, but if overproduced in the body, autoimmune diseases can result. [0003] Inflammatory factor storm (cytokine storm) refers to a variety of inflammatory factors in body fluids such as TNF-α, IL-1, IL-6, IL-12, IFN-α, IFN-β, IFN-γ after the body is infected with microorganisms , MCP-1 and IL-8, etc. are produced rapidly and in large quantities. Inflammatory...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/26B01J20/30A61M1/36
Inventor 姜建明李设桥
Owner 佛山市博新生物科技有限公司
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