A kind of preparation method of the phospholipid mainly composed of phosphatidylethanolamine

Abstract

The invention discloses a preparation method of phospholipid using phosphatidyl ethanolamine as a major ingredient. The method comprises the following steps of using soybean phospholipid powder or deoiling yolk powder as a raw material; performing extraction and aluminum oxide treatment to obtain aluminum oxide adsorbed with phosphatidyl ethanolamine; then, adding a mixed solvent consisting of organic solvent and organic alkali into the aluminum oxide adsorbed with phosphatidyl ethanolamine; performing desorption to obtain a solution containing phosphatidyl ethanolamine; obtaining the phospholipid using phosphatidyl ethanolamine as the major ingredient through concentration and drying. The content of the phosphatidyl ethanolamine in the obtained phospholipid product is not lower than 50 percent.

Description

technical field [0001] The invention relates to a method for preparing phospholipids with phosphatidylethanolamine as the main component, and belongs to the technical field of pharmaceutical engineering. [0002] technical background [0003] Phosphatidylethanolamine (referred to as PE), also known as ethanolamine phosphoglyceride and cephalin, is a good nutritional supplement, emulsifier, antioxidant, and can be used clinically as a hemostatic drug and a reagent for liver function tests. Recent studies have shown that oral administration of phosphatidylethanolamine can be directly utilized by the human brain to repair nerve cell membranes and restore normal metabolism of neurons. Phosphatidylethanolamine is also a natural emulsifier, which is widely used in the food industry and cosmetic industry. Phospholipids with different phosphatidylethanolamine contents have different emulsifying properties. In addition, it is also an important liposome auxiliary material such as synth...

AI Technical Summary

Problems solved by technology

[0007] There are few patents and documents on obtaining phosphatidylethanolamine by desorption from alumina. One of them, Japanese Patent JP10265485A, discloses that mixed phospholipids are used as raw materials, and ethanol containing ammonia is used as an extractant to obtain phosphatidylethanolamine content of 64.5%. , the method selects ethanol and ammonia water as solvents. During the desorption process, phosphatidylethanolamine is prone to hydrolysis and makes the product unstable.

At the same time, "Process Optimization of Separation and Purification of Phospholipids by Alumina Column Chromatography" (Master's Thesis of Zhejiang University, 2006) published by Liu Ye also reported that chloroform-methanol-ammonia water and chloroform-methanol-triethylamine were used for washing. The method of deoxidizing phosphatidylethanolamine in alumina, the research results show that adding amines in the elution solvent is beneficial to the elution of phosphatidylethanolamine in alumina, but its research also shows that phosphatidylethanolamine is in the chloroform-methanol-ammonia Or decomposition under the chloroform-methanol-triethylamine system leads to a significant decrease in PE content, which is not conducive to industrial production

[0008] Chinese Patent Grant No. CN100336818C discloses that passivated alumina is used as a filler, and methanol is used as an eluent for chromatographic separation to obtain more than 70% of phosphatidylethanolamine. After passivation, the adsorption capacity of phosphatidylethanolamine is weak, and the ability of passivated alumina to distinguish phosphatidylethanolamine and phosphatidylcholine is poor. This method does not use large-scale enrichment to purify phosphatidylethanolamine.

[0009] The above-mentioned several patents and literature reports show that the existing technology is not ideal for the large-scale enrichment and purification of phosphatidylethanolamine, and it is difficult to guarantee the yield and quality of the product.