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A kind of decellularized anti-calcification heart patch and preparation method thereof

A decellularized and cardiac technology, applied in the field of biomaterials of tissue engineering, can solve the problems of prone to calcification, affect safety, prolong material degradation time, etc., to achieve beneficial growth, reliable safety, and reduce the risk of calcification. Effect

Active Publication Date: 2018-01-23
SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, both similar heart patch products and pericardial materials treated by doctors before surgery are treated with glutaraldehyde to reduce the immunogenicity of raw materials, but glutaraldehyde has high toxicity, and the pericardium treated with glutaraldehyde During the degradation process of the material in vivo, glutaraldehyde molecules will be released when the collagen cross-linked by glutaraldehyde in the material, which affects the safety of the operation; at the same time, the glutaraldehyde-treated pericardial material has Changes in the collagen structure can easily lead to the formation of scar tissue after implantation, and the degradation time of the material is prolonged, making it easier to calcify

Method used

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  • A kind of decellularized anti-calcification heart patch and preparation method thereof
  • A kind of decellularized anti-calcification heart patch and preparation method thereof
  • A kind of decellularized anti-calcification heart patch and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of acellular and anti-calcified bovine pericardial heart patch

[0032] Step 1. Pretreatment of pericardial material: After removing blood stains and fatty tissue on the surface of fresh bovine pericardial material, add isopropanol at a mass ratio of 1:10, shake at 120 rpm for 12 hours, and rinse with purified water.

[0033] Step 2. Decellularization and anti-calcification treatment: Place the bovine pericardium obtained in Step 1 in a 3M sodium chloride mixed solution of 0.5% SDS and 0.75% sodium hydroxide, and shake treatment at 120 rpm for 10 minutes at 37°C, and then subject it to The purified water was oscillated at 120 rpm for 90 minutes, which was used as a cycle for a total of 3 cycles, and finally washed with purified water to neutrality.

[0034] Step 3. Terminal sterilization: use 15KGy of Co 60 Irradiate the bovine pericardium obtained in step two.

[0035] The bovine pericardium material has a denser structure. In this example, a higher conc...

Embodiment 2

[0036] Example 2: Preparation of acellular anti-calcification porcine pericardial heart patch

[0037] Step 1. Pretreatment of pericardial material: After removing blood stains and fatty tissue on the surface of fresh porcine pericardial material, add acetone at a mass ratio of 1:15, shake at 120rpm for 12 hours, and wash with purified water.

[0038] Step 2. Decellularization and anti-calcification treatment: Put the porcine pericardium obtained in step 1 in a 1.5M sodium chloride mixed solution of 0.3% SDS and 0.5% sodium hydroxide. At 37°C, shake at 100 rpm for 10 minutes, and then The purified water was oscillated at 100 rpm for 60 minutes, which was used as a cycle, and the total cycle treatment was 2 times, and finally the purified water was washed to neutrality.

[0039] Step three, terminal sterilization: use 100keV electron beam to process the porcine pericardium obtained in step two.

[0040] Porcine pericardium has a looser structure and higher fat content than bovine peric...

Embodiment 3

[0042] Step 1. Pretreatment of pericardial material: After removing blood stains and fatty tissue on the surface of fresh bovine pericardial material, add acetone at a mass ratio of 1:5, shake at 120rpm for 16 hours, and wash with purified water.

[0043] Step 2. Decellularization and anti-calcification treatment: Place the bovine pericardium obtained in step 1 in a 2.5M sodium chloride mixed solution of 0.2% SDS and 0.4% potassium hydroxide, and shake for 20 minutes at 130 rpm at 37°C, and then The purified water was oscillated at 130 rpm for 90 minutes, which was used as a cycle, and a total of 2 cycles were performed, and finally the purified water was washed to neutrality.

[0044] Step three, terminal sterilization: use ethylene oxide to sterilize the bovine pericardium material obtained in step two for 8 hours.

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Abstract

The invention relates to a preparation method of a decellularized anti-calcification heart patch. It mainly includes that raw pericardial tissue is degreased with organic reagents, treated with a mixed solution of high salt, surfactant and alkali, and finally sterilized to obtain a decellularized anti-calcification heart patch. The heart patch prepared by the present invention retains the three-dimensional pore structure of the collagen fibers of the raw material due to its special decellularization and anti-calcification process, and at the same time effectively removes the cells contained in the material itself, reducing immunogenicity; the material can guide autologous Cells grow in, reduce the formation of scar tissue, and have good anti-calcification ability; have excellent mechanical properties, can resist the arteriovenous pressure difference between the ventricles, and ensure the repair effect. The invention is suitable for atrial septal defect, ventricular septal defect and aortic stenosis caused by congenital heart disease.

Description

Technical field [0001] The invention belongs to the biomaterial technology of tissue engineering, and specifically relates to a decellularized anti-calcification heart patch and a preparation method thereof. Background technique [0002] According to statistics, there are more than 2 million patients with congenital heart disease in my country, and about 160,000 children with congenital heart disease are born every year, and 80% of the children need to use a patch for heart repair surgery. [0003] Since the heart defects caused by congenital heart disease are mainly symptoms of atrial septal defect, ventricular septal defect and patent ductus arteriosus, the heart repair material has certain performance requirements: 1) Good mechanical properties to resist the atrial or ventricular 2) Degradability. Because the heart is rich in blood, the patch will complete the fiber wrapping about 1 week after the patch is placed in the repair site. At this time, the patch has completed the prev...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 耿阳雪
Owner SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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