Lactobacillus fermentum Lee capable of preventing gastric ulcers and health-care application of lactobacillus fermentum Lee
A technology of Lactobacillus fermentum and gastric ulcer, applied in the field of microorganisms, to achieve the effect of reducing the area of gastric ulcer, reducing the degree of gastric ulcer, and preventing gastric ulcer
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0051] Example 1: Isolation, purification and preliminary identification of Lactobacillus fermentum Lee.
[0052] This embodiment is carried out according to the following steps:
[0053] (1) Separation and purification of Lactobacillus fermentum Lee.
[0054] Aseptically draw 500 μL of traditional fermented yak yogurt sample and add it to 5 mL sterilized saline to make a uniform dilution of 1:10, and continue to make a certain proportion of dilution. Select the appropriate gradient diluent, draw 100 μL each into the MRS solid plate medium with a sterile pipette tip, and incubate at 30°C for 48-72 hours, observe and record the colony morphology as follows: figure 1 shown.
[0055] Use an inoculation loop (or a sterilized toothpick) to pick different colonies from the surface and interior of the plate and inoculate them in the MRS liquid medium, and culture them on a shaker at 30°C and 300r / min for 24-48h; repeat the above steps for continuous activation for 2 generations bef...
Embodiment 2
[0061] Example 2: In vitro screening of Lactobacillus fermentum Lee.
[0062] (1) Screening of probiotics resistant to pH 3.0 artificial gastric juice.
[0063] Preparation of artificial gastric juice: 0.2% NaCl, 0.35% pepsin, adjust the pH value to 3.0 with 1M HCl, filter and sterilize with a vacuum pump in an aseptic operating table for later use.
[0064] Determination of the tolerance of probiotics to artificial gastric juice: take 5mL of the activated strain culture solution, pour it into a sterilized 10mL centrifuge tube in a sterile operating table, collect the bacteria by centrifugation at 3000r / min for 10min, add 5mL of sterilized Mix normal saline to make bacterial suspension, mix 1mL bacterial suspension with 9mL pH3.0 artificial gastric juice, shake well, place in a constant temperature shaker (37°C, 300r / min), and take samples at 0h and 3h respectively , with MRS agar medium poured 37 ° C for 48h. The number of viable bacteria was determined by plate counting me...
Embodiment 3
[0078] Example 3: Experiment of gastric ulcer induced by reserpine.
[0079] Feed 50 healthy SPF grade Kunming mice, weighing 23±2g, male. After being fed with basic feed for 7 days, the mice were randomly divided into 5 groups according to body weight, namely: normal group, gastric ulcer control group, omeprazole group, Lactobacillusbulgaricus group and LactobacillusfermentumLee group, with 10 mice in each group. Raise them in stainless steel cages, keep room temperature at 24±2°C, relative humidity at 50±10%, and light-dark rotation for 12 hours (lighting at 8:00-20:00). During the whole experimental period, the normal group and the gastric ulcer control group were fed with 0.2mL normal saline every day, and the experimental mice in the Lactobacillusbulgaricus group and the LactobacillusfermentumLee group were fed with 1.0×10 9 CFU / kg gavage 0.2mL of the corresponding bacterial solution. The omeprazole group was orally administered 0.2mL of omeprazole diluent at 30 mg / kg p...
PUM
| Property | Measurement | Unit |
|---|---|---|
| hydrophobicity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More