A gene chip for detecting marine pathogenic vibrio and its preparation method and detection method

A technology of gene chip and pathogenic Vibrio, which is applied in the field of gene chip for detecting marine pathogenic Vibrio and its preparation field, can solve the problems of tools that are not easy to monitor pathogenic bacteria, increase economic burden, take time and so on, and achieve wide application , Simple operation, good repeatability
CN105133040BActive Publication Date: 2018-01-23浙江正合谷生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
浙江正合谷生物科技有限公司
Publication Date
2018-01-23

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Abstract

The invention discloses a gene chip detecting marine pathogenic vibrios, and a preparation method and a detection method thereof. The gene chip comprises a solid-phase carrier and detection probes fixedly disposed on the solid-phase carrier, and the detection probes comprise vibrio vulnificus gyrB gene probe, vibrio vulnificus virulence gene hemA probe, vibrio splendidus gyrB gene probe, vibrio splendidus virulence gene toxR probe, vibrio harveyi gyrB gene probe, vibrio harveyi virulence gene hemA probe, vibrio parahaemolyticus gyrB gene probe, vibrio parahaemolyticus virulence gene toxS probe, vibrio anguillarum gyrB gene probe and vibrio anguillarum virulence gene hem probe which are respectively shown as SEQ NO 1-10. The gene chip is firstly applied to detection on marine pathogenic vibrios. The gene chip is capable of rapidly sensitively detecting target bacterium infectio, also is good in repeatability and strong in signal, and does not easily cause a nonspecific signal.
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Description

technical field

[0001] The invention belongs to the technical field of gene chip detection, and in particular relates to a gene chip for detecting marine pathogenic vibrio, a preparation method and a detection method thereof. Background technique

[0002] Pathogenic bacterial infection is a thorny problem faced by human beings, which often results in high mortality and disability rates. Early diagnosis and treatment are of great significance. Delayed diagnosis leads to blind application of antibacterial drugs, increases economic burden, and promotes the occurrence of bacterial drug resistance. The culture method is currently the gold standard for the diagnosis of bacterial infection, but it takes a long time and is often diagnosed based on biochemical reactions and other phenotypes. It is easy to misdiagnose and it is not easy to be used as a tool for monitoring pathogenic bacteria.

[0003] Biochip technology is a technology that has developed rapidly in the field of biolo...

Claims

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