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Application of lachnum water-soluble melanin SLIM404 in raising immunity

A technology of Trichomyces granulosa and melanin, which is applied in the field of water-soluble Trichomyces granulosa melanin SLIM404, to achieve the effects of improving immune drugs, improving immunity, and good economies of scale

Inactive Publication Date: 2015-12-23
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no public documents at home and abroad that mention the use of Trichomonas melanin as an application of immunomodulatory drugs

Method used

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  • Application of lachnum water-soluble melanin SLIM404 in raising immunity
  • Application of lachnum water-soluble melanin SLIM404 in raising immunity
  • Application of lachnum water-soluble melanin SLIM404 in raising immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of intracellular melanin (LIM) of Trichomonas granulosa:

[0022] Trichodia granulosa YM404 (the strain number is derived from the literature Purification, structure and anti-radiation activity of melanin from Lachnum YM404. International journal of biological macromolecules, 2014, 63:170-176. Periodical ISSN: 0141-8130) was inoculated in a 100L fermenter, and the medium formula was: tyrosine Concentration is 1.0mmol / L, copper ion concentration is 1.1mmol / L, sucrose mass is 25.5g / L, yeast extract is 5.0g / L, peptone is 5.0g / L, pH8.0, loading coefficient volume ratio is 0.7, inoculation The amount is 5% (v / v), the culture temperature is 27°C, the ventilation rate is 1.0L / min, the stirring speed is 180r / min, and the fermentation time is 12d; dry, according to the mass of mycelium (g) and the concentration of 1mol / L sodium hydroxide (mL) ratio of 1:100, mix well, stand at 30°C for 12h, and suction filter to obtain the extract; Add 1 mol / L HCl to adjust the p...

Embodiment 2

[0085] Processing of water-soluble melanin preparation from Trichomonas granulosa

[0086] 1. Tablet processing

[0087] Mix 200-400g of Trichodia melanin with 200-250g of pharmaceutical excipient diluent and 20-60g of glidant, and then mix with 250-500mL of ethanol to obtain soft material, and sieve the soft material to obtain wet granules. After drying, granulate, and finally add 5-10g of lubricant, mix well, and then compress into tablets to make 1000 tablets. The pharmaceutical excipient diluent is dextrin or dry starch; the glidant is talcum powder or micropowder silica gel or microcrystalline cellulose; the lubricant is magnesium stearate or zinc stearate.

[0088] The preferred example of tablet processing recommended in this embodiment is: take 500g of the above-mentioned Trichomonas melanin, 250g of diluent dextrin, and 60g of glidant talcum powder that have passed through a 80-100 mesh sieve, mix well and add 500mL of ethanol to mix well Prepare the soft material, ...

Embodiment 3

[0094] First prepare and obtain the intracellular melanin of Trichodia granulosus according to the following conditions and methods: Inoculate Trichodia granulosa YM404 in a 100L fermenter, the medium formula is: tyrosine concentration is 1.2mmol / L, copper ion concentration is 1.2mmol / L L, sucrose quality is 30g / L, yeast extract 5.0g / L, peptone 5.0g / L, pH8.0, loading factor volume ratio is 0.7, inoculum size is 7% (volume ratio), culture temperature is 25 ℃, The ventilation rate is 1.2L / min, the stirring speed is 180r / min, and the fermentation time is 15 days; the fermentation broth is suction-filtered, the mycelium is collected, dried at 60°C, and the mass (g) and concentration of the mycelium are 1.5mol The ratio of sodium hydroxide (mL) per L is 1:150, mixed evenly, leached in a water bath at 30°C for 12 hours, and suction filtered to obtain the extract; adjust the pH value of the above extract to 2 with 1mol / L HCl, and let it stand for 20 hours to obtain Melanin suspension...

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Abstract

An application of lachnum water-soluble melanin SLIM404 in raising immunity. The invention belongs to the field of immunity in medicine and provides lachnum melanin SLIM404 and specifically relates to an application of the melanin in raising immunity and in preparing medicines for raising immunity. The melanin is intracellular melanin of lachnum YM404 and is a water-soluble lachnum melanin obtained after sulfur trioxide-pyridine modification. The melanin has an effect of raising immunologic function, and raises immunity by promoting thymus organ development, raising phagocytosis percentage and phagocytic index of macrophage, enhancing B cell-mediated humoral immunity, promoting a T cell-mediated immune response, increasing activity of an in-vivo antioxidant enzymatic system and removing excess free radicals and lipid peroxide in the immunocompromised body and the like. A medicine prepared from the melanin has various routes of administration, is safe and nontoxic, is easy to prepare and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention belongs to the field of medicine and immunity, and relates to a water-soluble trichomona melanin SLIM404, in particular to the application of the melanin in improving immunity and preparing immunity-improving medicines. Background technique [0002] The immune system is the body's response to foreign species, foreign bodies and its own substances, and the tissue system that exerts immune functions. Structurally, the immune system can be divided into immune organs, immune factors and immune cells, and functionally, the immune system can be divided into non-specific immunity and specific immunity. A series of life processes such as embryonic development, disease occurrence, and aging in the human body are closely related to the immune system. The body's self-protection depends on this perfect immune system. A healthy human body needs to maintain a reasonable and balanced immune system. [0003] Melanin generally exists in animals, plants a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/06A61P37/04
Inventor 叶明宋升李兰徐灿李盛兰杨柳
Owner HEFEI UNIV OF TECH
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