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Bacillus amyloliquefaciens for controlling tomato gray mould and application of bacillus amyloliquefaciens

A technology for dissolving starch spores and tomato botrytis, which is applied in the field of agricultural biological control and can solve problems such as environmental pollution and harmful pests.

Active Publication Date: 2015-12-30
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical control has the advantages of quick effect and high efficiency, it is gradually eliminated due to the disadvantages of environmental pollution and natural enemies of pests caused by long-term use; the second is biological control, which has high efficacy, long duration and is not easy to control. The advantages of drug resistance and environmental friendliness are increasingly favored by people

Method used

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  • Bacillus amyloliquefaciens for controlling tomato gray mould and application of bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens for controlling tomato gray mould and application of bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens for controlling tomato gray mould and application of bacillus amyloliquefaciens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The preparation of embodiment 1HMB27633 microbial inoculum

[0055] Follow these steps:

[0056] (1) Activation of strains: The strain HMB27633 (Bacillus amyloliquefaciens strain HMB27633) stored at -80°C was deposited in the General Microbiology Center of the China Microorganism Culture Collection Management Committee on September 29, 2015 (preservation address: Chaoyang, Beijing District Beichen West Road No. 1, No. 3, Institute of Microbiology, Chinese Academy of Sciences), the preservation number is CGMCCNo.11454) in LB plate medium (its composition and its weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), carry out activation (30 ℃), pick single colony LB slant medium (its composition and its weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, 15 g of agar powder, 1000 mL of water) and cultivated at 30° C. for 12 hours to obtain activated strains;

[0057] (2) Preparation of seed liquid: make L...

Embodiment 2

[0060] Example 2 Antagonism test of Bacillus amyloliquefaciens HMB27633 on tomato Botrytis cinerea

[0061] (1) Test time and place: In early August 2013, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry.

[0062] (2) Test method:

[0063] (1) Source of tomato gray mold for the test: Tomato gray mold BC-1 strain was collected from tomato diseased fruit in Dongniu Dongzhuang Village, Rongcheng County, Baoding City, Hebei Province. The plant pathology department of the college identified it as Botrytis cinerea, and the pathogenicity test showed that it was highly pathogenic.

[0064] (2) Plate confrontation experiment:

[0065] First, tomato Botrytis cinerea BC-1 was activated on PDA plates, and after 4 days of culture, in the edge area of ​​the colony, a hole punch was used. Punch holes to make bacterial slices, transfer tomato Botrytis cinerea slices in the center of anothe...

Embodiment 3

[0069] Example 3 Comparative test of control effect of Bacillus amyloliquefaciens HMB27633 on tomato gray mold

[0070] (1) Test treatment:

[0071] (1) Microbial inoculum: HMB27633 liquid preparation prepared in Example 1; diluted 50 times with water.

[0072] (2) Chemical agent: azoxystrobin suspending agent (250 g / L) (Syngenta Ltd., UK); diluted 500 times with water.

[0073] (3) Blank control: clear water

[0074](2) Test method: This test was carried out in the Plant Disease Biocontrol Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry. Tomato seedlings (tomato L-402, produced by Liaoning Horticultural Seedling Company, Liaoning Academy of Agricultural Sciences, Institute of Horticulture, Liaoning Academy of Agricultural Sciences) were cultivated in a 50-hole seedling tray, and transplanted into flowerpots with a diameter of 20 cm when 2 compound leaves grew. Pot 1 seedling. When the plant grows to 7-8 compound leaves, select le...

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Abstract

The invention belongs to the field of agricultural biological control and particularly discloses a bacillus amyloliquefaciens strain HMB27633 which is used for controlling tomato gray mould and has the preservation number of CGMCC No.11454. The invention further discloses a microbial agent prepared from bacillus amyloliquefaciens and an application of the bacillus amyloliquefaciens to control of the tomato gray mould. The bacillus amyloliquefaciens HMB27633 is high in bacterium inhibiting activity, has the good control effect on the tomato gray mould and has the lasting and stable antibacterial effect; secondarily, the microbial agent is safe to humans and livestock and is environment-friendly; besides, drug resistance is not produced easily when the tomato gray mould is controlled with the method; in addition, the bacillus amyloliquefaciens HMB27633 is high in stress resistance, the preparation method is simple, the cost is low, and the bacillus amyloliquefaciens HMB27633 is convenient to use.

Description

technical field [0001] The invention belongs to the field of agricultural biological control, in particular to a Bacillus amyloliquefaciens for preventing and treating tomato gray mold, a microbial inoculum containing the Bacillus amyloliquefaciens, and their use in preventing and controlling tomato gray mold. Background technique [0002] Tomato gray mold is an important disease caused by Botrytis cinerea Pers. The disease mainly damages the fruit, causing the fruit to rot, and also damages the leaves, stems and flowers. The tomato yield can be reduced by 20% to 50%, and the loss is serious. The pathogen has a wide range of hosts. In addition to infecting Solanaceae vegetables, it can also infect crops such as melons, cabbage, kidney beans, lettuce, onions, and apples. [0003] At present, the main control methods for tomato gray mold are as follows: First, chemical control. Although chemical control has the advantages of quick effect and high efficiency, it is gradually ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12Q1/68C12Q1/04C12R1/07
Inventor 鹿秀云马平李社增郭庆港张晓云商俊燕
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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