A kind of ochratoxin up-conversion fluorescent sensing material and preparation method thereof
An ochratoxin and sensing material technology, which is applied to the field of ochratoxin up-conversion fluorescent sensing materials and their preparation, can solve the problems of inability to identify targets, lack of specificity of UCNPs, etc., and achieves easy popularization, convenient material preparation, low cost effect
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Embodiment 1
[0023] The invention provides a preparation method of ochratoxin up-conversion nano-fluorescence sensing material, the specific preparation method is as follows:
[0024] (1) Weigh 700mg of sodium hydroxide and dissolve it in 5mL of water and 8mL of ethanol, dissolve completely at room temperature, then add 8mL of oleic acid to form a slightly yellow clear solution; add 2.5mL of yttrium nitrate, ytterbium nitrate and nitric acid to the above solution Erbium mixture (0.4mol L -1 ), to obtain a milky white thickened solution; after 10min, 247mg NH was added dropwise 4 20mL of ethanol + water (1+1, v / v) in F was stirred for 10min and ultrasonicated; the solution was transferred to a polytetrafluoroethylene-lined high-temperature reactor, reacted at 220°C for 12h, cooled to room temperature, and centrifuged. , washed with ethanol;
[0025] (2) Weigh 100 mg of the up-converting nanoparticles prepared above and dissolve in 100 mL Triton X-100+H 2 O(1+4, v / v), react for 8 hours, c...
Embodiment 2
[0030] The adsorption performance of the sensing material was studied. Add 1mg of UCNPs@MIP or UCNPs@NIP into a 4mL centrifuge tube, then add 2mL with concentrations of 0.2, 0.4, 0.6, 0.8, 1.0, 2.0, 2.5, 4.0, 8.0, 10.0mg L -1 ochratoxin standard solution. The mixture was shaken at room temperature for a period of time, and then its fluorescence value was measured with a fluorescence spectrophotometer. As the concentration of ochratoxin increased, the fluorescence intensity weakened, and at the same concentration, the fluorescence quenching of UCNPs@MIP was greater than that of UCNPs@NIP.
[0031] Example 2
[0032] The dynamic performance of the sensing material was studied. Weigh 1 mg of UCNPs@MIP prepared in Example 1 and place it in a 4 mL centrifuge tube, then add 2 mL of 1 ppm ochratoxin A, shake at room temperature for 0.5, 1, 1.5, 2, 2.5, 2, 2.5, 4, After 4.5 hours, measure the fluorescence value with a fluorescence spectrophotometer connected with an external 980nm...
Embodiment 4
[0034] Select Aflatoxin B 1 (AFB 1 ) is a competitor of ochratoxin A (OTA), and the selectivity of the sensing material is studied. Prepare the concentration of 1.0mg L respectively -1 AFB 1Single standard solution with OTA and mixed solution of both. Take 2 mL of the prepared solution and add it to a centrifuge tube, add 1 mg of UCNPs@MIP and UCNPs@NIP respectively, shake at room temperature for 2 hours, and then measure the fluorescence value with a fluorescence spectrophotometer connected to an external 980nm exciter. It shows that UCNPs@MIP has selective recognition for the target toxin. OTA has a significant quenching effect on the fluorescent imprinted polymer material UCNPs@MIP, while the competitor AFB 1 The quenching effect on it is relatively weak; OTA and AFB 1 The degree of quenching of the fluorescent non-imprinted polymeric material UCNPs@NIP is very small. In the presence of AFB1, the degree of quenching of UCNPs@MIP by OTA was comparable to that of OTA a...
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