A bioactive hard tissue engineering scaffold material and preparation method thereof

A bioactive scaffold material technology, applied in the field of hard tissue engineering scaffold materials and its preparation, can solve the problems of not being able to repair bone defects in weight-bearing parts, destroying the natural three-dimensional structure, and being expensive, and achieving good biocompatibility and degradation Sexuality, good application prospects, and high biological safety

Active Publication Date: 2018-09-11
BEIJING DATSING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1. Simple ceramic substitute materials have high brittleness and low toughness, and cannot be used for the repair of bone defects in load-bearing parts
[0010] 2. The current research mainly focuses on the preparation of collagen scaffolds by extracting collagen and then cross-linking, which is expensive and destroys the natural three-dimensional structure of collagen, making it difficult to achieve the mechanical and biological properties of bone substitute materials
At the same time, during the process of dissolving collagen, the terminal peptide is likely to be destroyed, thereby causing antigenicity of collagen and affecting the growth of tissue cells
[0011] 3. The way of physical mixing of inorganic substances and organic substances is not stable in the body, which is far from the structure of human hard tissues

Method used

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  • A bioactive hard tissue engineering scaffold material and preparation method thereof
  • A bioactive hard tissue engineering scaffold material and preparation method thereof
  • A bioactive hard tissue engineering scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 support material

[0037] Preparation of porcine small intestinal submucosa: the fresh porcine small intestinal submucosa was disinfected with peracetic acid or alcohol, after disinfection, the porcine small intestinal submucosa was sequentially immersed in sodium hydroxide and EDTA, and then washed repeatedly with deionized water to obtain the ECM material.

[0038] Soak the prepared ECM material in Tris buffer solution with pH = 8.8, put it in a shaker at 37°C to make it fully swell for 12 hours, take it out and rinse it with deionized water for 3 times, each time for 10 minutes; then dry it with filter paper The surface moisture was quickly frozen in liquid nitrogen for 2 hours, and vacuum freeze-dried at -80°C for 8 hours to obtain a fully expanded collagen scaffold. The collagen scaffold: a) Soak in 25ml 0.2M CaCl 2 solution, and shake it at a speed of 120 rpm in a shaker at 20°C for 1 hour, take it out and wash it with deionized wa...

Embodiment 2

[0040] The preparation of embodiment 2 support material

[0041] Preparation of bovine pericardium matrix: fresh bovine pericardium was sterilized with peracetic acid or alcohol, after disinfection, the bovine pericardium was immersed in sodium hydroxide and EDTA in sequence, and then washed with deionized water repeatedly. Get the ECM material.

[0042] Soak the prepared ECM material in Tris buffer solution with pH=8.8 (preparation method is to add 121.1g Tris to 800ml purified water, add concentrated HCL to adjust the pH value to 8.8, continue to add water to 1 liter), shake at 37°C Let it fully swell in the bed for 24 hours, take it out and wash it with deionized water for 3 times, 10 minutes each time; then dry the surface moisture with filter paper, put it into liquid nitrogen and freeze it for 2 hours, and vacuum freeze-dry it at -80°C for 8 hours , to obtain fully expanded collagen scaffolds. The collagen scaffold: a) soaked in 25ml 0.1M CaCl 2 solution, and shake it...

Embodiment 3

[0043] The preparation of embodiment 3 support material

[0044] Preparation of bovine leather matrix: fresh bovine leather is sterilized with peracetic acid or alcohol, after disinfection, the bovine leather is immersed in petroleum ether and sodium hydroxide in sequence, and then washed with deionized water repeatedly. Get the ECM material.

[0045] Soak the prepared ECM material in PBS buffer solution with pH = 8.8, place it in a shaker at 37°C to fully swell for 36 hours, take it out and wash it with normal saline 10 times, 10 minutes each time; then dry the surface moisture with filter paper , placed in -40°C for rapid freezing for 12 hours, and vacuum freeze-dried at -80°C for 8 hours to obtain fully expanded collagen scaffolds. The collagen scaffold: a) Soak in 25ml 0.3M CaCl 2 solution, and shake it at a speed of 120 rpm for 4 hours in a shaker at 25°C, take it out and wash it with normal saline for 5 times, each time for 5 minutes; b) soak it in 25ml of 0.5M (NH 4 ...

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Abstract

The invention provides a hard tissue engineering scaffold material having a bioactivity and a preparation method of the hard tissue engineering scaffold material. The preparation method comprises the following steps: (1) fully swelling ECM (extracellular matrix), which is subject to antigen extracting treatment, in a Tris or PBS buffer solution, flushing after swelling, and freeze-drying so as to obtain a collagen scaffold; (2) repeatedly soaking the collagen scaffold in a solution containing Ca2+ and a solution containing (PO4)3- in turn and conducting a mineralization reaction, then flushing and freeze-drying so as to obtain a composite scaffold material; and (3) soaking the composite scaffold material in one or more composite solutions of a chitosan solution, a chondroitin sulfate solution, a sodium hyaluronate solution and a skeletal growth factor solution, and freeze-drying so as to obtain the scaffold material having the bioactivity. The hard tissue engineering scaffold material disclosed by the invention is closer to the structure of human bone; and the hard tissue engineering scaffold material is good in bioactivity, simple in preparation process, relatively low in cost and good in application prospect.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a biologically active hard tissue engineering scaffold material and a preparation method thereof. Background technique [0002] Biomedical materials are materials used to combine with biological systems, treat or replace tissues and organs in biological organisms or enhance their functions. As a class of artificial or natural materials, it can be made into finished products alone or together with medicines for the treatment or replacement of tissues or organs, with the ultimate goal of being able to replace or repair human organs and tissues and realize their physiological functions. Biomedical materials are divided into medical metal materials, medical polymer materials, bioceramic materials and biomedical composite materials according to material composition and properties. According to the application, it can be divided into hard tissue repair and replacement materi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/12A61L27/20A61L27/22A61L27/54A61L27/58
Inventor 代金明马淑金丁力李湘杰牛睿李次会
Owner BEIJING DATSING BIO TECH
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