A bioactive hard tissue engineering scaffold material and preparation method thereof

Abstract

The invention provides a hard tissue engineering scaffold material having a bioactivity and a preparation method of the hard tissue engineering scaffold material. The preparation method comprises the following steps: (1) fully swelling ECM (extracellular matrix), which is subject to antigen extracting treatment, in a Tris or PBS buffer solution, flushing after swelling, and freeze-drying so as to obtain a collagen scaffold; (2) repeatedly soaking the collagen scaffold in a solution containing Ca2+ and a solution containing (PO4)3- in turn and conducting a mineralization reaction, then flushing and freeze-drying so as to obtain a composite scaffold material; and (3) soaking the composite scaffold material in one or more composite solutions of a chitosan solution, a chondroitin sulfate solution, a sodium hyaluronate solution and a skeletal growth factor solution, and freeze-drying so as to obtain the scaffold material having the bioactivity. The hard tissue engineering scaffold material disclosed by the invention is closer to the structure of human bone; and the hard tissue engineering scaffold material is good in bioactivity, simple in preparation process, relatively low in cost and good in application prospect.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a biologically active hard tissue engineering scaffold material and a preparation method thereof. Background technique [0002] Biomedical materials are materials used to combine with biological systems, treat or replace tissues and organs in biological organisms or enhance their functions. As a class of artificial or natural materials, it can be made into finished products alone or together with medicines for the treatment or replacement of tissues or organs, with the ultimate goal of being able to replace or repair human organs and tissues and realize their physiological functions. Biomedical materials are divided into medical metal materials, medical polymer materials, bioceramic materials and biomedical composite materials according to material composition and properties. According to the application, it can be divided into hard tissue repair and replacement materi...

AI Technical Summary

Problems solved by technology

[0009] 1. Simple ceramic substitute materials have high brittleness and low toughness, and cannot be used for the repair of bone defects in load-bearing parts

[0010] 2. The current research mainly focuses on the preparation of collagen scaffolds by extracting collagen and then cross-linking, which is expensive and destroys the natural three-dimensional structure of collagen, making it difficult to achieve the mechanical and biological properties of bone substitute materials

At the same time, during the process of dissolving collagen, the terminal peptide is likely to be destroyed, thereby causing antigenicity of collagen and affecting the growth of tissue cells

[0011] 3. The way of physical mixing of inorganic substances and organic substances is not stable in the body, which is far from the structure of human hard tissues

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