Culture solution composition capable of efficiently culturing chlorella

A technology of culture fluid and composition, which is applied in the field of culture fluid composition of chlorella, and can solve problems such as difficulty in cultivating ultra-high cell concentration of chlorella

Active Publication Date: 2016-02-10
福建格林生物科技有限公司 +1
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Problems solved by technology

[0005] The purpose of the present invention is to screen out chlorella species capable of heterotrophic growth, and to determine the optimal culture control conditions for obtaining ultra-high cell concentration ch

Method used

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Examples

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Embodiment 1

[0018] At room temperature (20-25°C), take 1 g of naphthalene acetic acid, 5,290 g of urea, 300 g of sodium nitrate, 295 g of anhydrous calcium chloride, 750 g of magnesium sulfate heptahydrate, 360 g of dipotassium hydrogen phosphate, 60 g of ferric ammonium citrate, and 60 g of citric acid , Anhydrous sodium carbonate 200g, edetate disodium 10g, boric acid 26.6g, manganese chloride tetrahydrate 18.1g, zinc sulfate heptahydrate 2.22g, sodium molybdate dihydrate 3.9g, copper sulfate pentahydrate 0.79g, Cobalt nitrate hexahydrate 0.49g. Pour it into a container filled with 10L of water, stir evenly, then take 1 liter of chlorella species with a concentration of 20-50g / L into the above water container, place it in the open air, and then, after 10 days of ventilation, it is tested that the above water The concentration of chlorella in the container reaches 50-70g / L.

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Abstract

The invention provides a culture solution composition capable of culturing chlorella. The culture solution composition is characterized by being prepared from, by weight, 0.01%-0.02% of naphthalene acetic acid, 70%-75% of urea, 3.5%-4.5% of sodium nitrate, 3.5%-4.5% of anhydrous calcium chloride, 9%-12% of magnesium sulfate heptahydrate, 4%-5% of dipotassium phosphate, 0.8%-0.9% of ammonium ferric citrate, 0.8%-0.9% of citric acid, 2%-3% of anhydrous sodium carbonate, 0.1%-0.2% of ethylenediaminetetraacetic acid disodium, 0.3%-0.4% of boric acid, 0.2%-0.3% of tetrahydrate manganese chloride, 0.02%-0.04% of zinc sulfate heptahydrate, 0.04%-0.06% of sodium molybdate dihydrate, 0.01%-0.02% of copper sulfate pentahydrate and 0.005%-0.007% of cobalt nitrate hexahydrate.

Description

technical field [0001] The invention relates to a chlorella culture medium composition and a preparation method thereof. Background technique [0002] In the current market, there are about 10 species of Chlorella in the genus Chlorella, all of which are rich in protein, unsaturated fatty acids, carotenoids, vitamins and chlorella factors and other important life active substances, and have extremely high nutrition The value and the function of improving immunity have been listed as the health food of human beings in the 21st century. With the improvement of people's living standards, the demand for chlorella will increase. [0003] It is generally believed that chlorella belongs to lower plants, mainly absorbing inorganic carbon and synthesizing organic matter through photosynthesis. However, when the photoautotrophic culture method is adopted, when the concentration of algae cells increases to a certain number, the light must be blocked from entering the culture, so that...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 刘元凯胡征宇
Owner 福建格林生物科技有限公司
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