Colchicine sublingual tablet, preparation method and application thereof
A technology of colchicine and sublingual tablet, applied in the field of colchicine, can solve the problem that the sublingual tablet does not have universal applicability and the like, and achieve the effects of avoiding liver first-pass effect, fast onset of action and reducing toxicity
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[0048] The present invention provides a kind of preparation method of colchicine sublingual tablet described in above-mentioned technical scheme, comprises the following steps:
[0049] Inclusion of colchicine with cyclodextrin to obtain an inclusion compound;
[0050] Mixing the clathrate, mannitol, lactose and hydroxypropyl cellulose in an equal-volume ascending method to obtain a mixture;
[0051] mixing the mixture with hydroxypropylmethylcellulose and water, followed by granulation and drying in sequence to obtain an intermediate product;
[0052] The intermediate product is mixed with magnesium stearate and compressed into tablets to obtain colchicine sublingual tablets.
[0053] In the present invention, colchicine is clathrated with cyclodextrin to obtain clathrate; the specific steps of clathrate preferably include:
[0054] dissolving colchicine in an alcoholic solvent to obtain a colchicine solution;
[0055] The colchicine solution and cyclodextrin aqueous solut...
Embodiment 1
[0074] Embodiment 1: Colchicine relative bioavailability research
[0075] Test drug:
[0076] Name: Colchicine Sublingual Tablets, Specification: 0.5mg; Provider: Kunming Pharmaceutical Group Co., Ltd.; Batch Number: 20140616;
[0077] Reference preparation: colchicine tablets, specification: 1mg; provider: Kunming Pharmaceutical Group Co., Ltd.; batch number: 20140320;
[0078] animal:
[0079] Source, species, strain: Beagle, provided by the Experimental Animal Center of the Academy of Military Medical Sciences, body weight: 10-13kg, sex: ♂, total number of animals: 8.
[0080] Method and Results:
[0081] Chromatographic conditions:
[0082] Chromatographic column: Kromasil C 18 (200mm×4.6mm, 5μm);
[0083] Mobile phase: formic acid: 10mmol / L ammonium acetate: methanol (1:44:55, V:V:V);
[0084] Flow rate: 1.0mL / min;
[0085] Injection volume: 20μL;
[0086] Detection wavelength: 245nm;
[0087] Column temperature: 30°C;
[0088] Internal standard: carbamazepine...
Embodiment 2
[0101] Dissolve the prescribed amount of colchicine in the ratio of 1:1mL in ethanol with a mass fraction of 60%, and add a 20% mass fraction of hydroxypropyl-β-cyclodextrin aqueous solution, colchicine and hydroxypropyl-β - The mass ratio of cyclodextrin is 1:5, stir for 2 hours, heat the solution in a water bath to 60°C, remove the solvent under reduced pressure, scrape out the clathrate, and dry in an oven at 60±2°C At constant weight, the inclusion compound was passed through an 80-mesh sieve to obtain the colchicine hydroxypropyl-β-cyclodextrin inclusion compound;
[0102] Colchicine Sublingual Tablets 1000 Tablets Prescription Size:
[0103]
[0104] Note: 6.7g of colchicine hydroxypropyl-β-cyclodextrin inclusion compound contains 0.5g of colchicine;
[0105] Process: mix colchicine hydroxypropyl-β-cyclodextrin inclusion compound, mannitol, lactose and HPC in an equal-volume ascending method, and pass through a 20-mesh sieve for 3 times to obtain the mixture;
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