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Monimopetalum Chinese extract with anti-inflammatory activity, preparing method and application of extract

A technology of vine and its extract, which is applied in the field of traditional Chinese medicine, can solve problems such as scarcity of vine resources, farmers' reluctance to plant it, and no economic benefits, etc., to reduce composition and side effects, improve drug efficacy, and simple preparation process Effect

Active Publication Date: 2016-04-20
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although forestry and agricultural scholars have found the reason for its low reproductive rate and have technology to improve its reproductive rate, but there is no economic benefit, farmers are unwilling to plant it, and cannot promote it, so the resources of the vine are still scarce.

Method used

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  • Monimopetalum Chinese extract with anti-inflammatory activity, preparing method and application of extract
  • Monimopetalum Chinese extract with anti-inflammatory activity, preparing method and application of extract
  • Monimopetalum Chinese extract with anti-inflammatory activity, preparing method and application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Preparation of the vine extract (total triterpene part).

[0033] Experimental method: 5kg of the stems and leaves of the vine petals, heated and refluxed with 8 times the amount of 80% ethanol at 80°C for 3 times, filtered, concentrated to a small volume, suspended with a small amount of water, and then subjected to D101 macroporous resin column chromatography to elute impurities with water (Fr.1), 20% ethanol elution fraction (Fr.2), 40% ethanol elution fraction (Fr.3) and 60% ethanol elution fraction (Fr.4) were collected separately, 80% ethanol and 100 The fractions eluted with % ethanol were combined (Fr.5 and Fr.6) to obtain the total triterpene fractions after collection, concentration and drying.

[0034] Component analysis of the total triterpene fraction.

[0035] Analysis method: 1260UPLC-6530QTOF liquid mass spectrometer from Agilent Company of the United States; chromatographic column: Agilent ZorbaxSB-C 18 600bar, 1.8μm, 4.6×100mm; mobile phas...

Embodiment 2

[0037] Example 2. Cytotoxicity test (MTT method) of extracts from various parts of Tenghua vine.

[0038] MTT method, also known as MTT colorimetric method, is a method for detecting cell survival and growth. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan (Formazan) and deposit in the cells, while dead cells do not have this function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its light absorption value is measured at a wavelength of 540-720nm with an enzyme-linked immunosorbent detector, which can indirectly reflect the number of living cells. Within a certain cell number range, the amount of MTT crystal formation is proportional to the cell number. This method has been widely used in the activity detection of some biologically active factors, large-scale antitumor drug screening, cytotoxicity test and tumor radiosensitivity determinati...

Embodiment 3

[0043] Example 3. The extracts from various parts of the vine of the vine have effects on the inflammatory factor prostaglandin E 2 (PGE 2 ) inhibition.

[0044] PGE 2 It is an extremely important lipid metabolite, which is released when subjected to various physiological or pathological stimuli, especially harmful stimuli, and plays an important role in fever, inflammation and blood pressure regulation. Establish an in vitro anti-inflammatory model using lipoprotein (LPS)-induced mouse macrophage RAW264.7, and detect PGE by enzyme-linked immunoassay (ELISA) 2 The lower the concentration of inflammatory factors, the higher the inhibition rate and the better the anti-inflammatory activity. The positive control drug was indomethacin (IM).

[0045]

[0046] Experimental results: It can be seen from Table 2 that both the 80% ethanol elution site (Fr.5) and the 100% ethanol elution site (Fr.6) of Echinocarpus can inhibit the PGE induced by LPS 2 produce.

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Abstract

The invention relates to the technical field of traditional Chinese medicine, and discloses monimopetalum Chinese extract with anti-inflammatory activity. The monimopetalum Chinese extract is an effective component formed by pristimerin, wilforol A, polpunonic acid, celastrol, tingenone, friedelin and other triterpene ingredients. A preparing method is characterized by including the steps that stems and leaves of the plant monimopetalum Chinese (Monimopetalum Chinese Rehd.) are used as raw materials and extracted with methyl alcohol, or ethyl alcohol or water-containing alcohol, an extracting solution is filtered and condensed to a small volume, a small amount of water is added to suspend the filtered and condensed extracting solution, then macroporous resin column chromatography is used, and 80-100% of an alcohol elution component is collected to obtain the total triterpene component. The preparing method is simple in process and reasonable in operation, and the extract has an obvious inhibiting effect on inflammatory mediators such as prostaglandin E2 and nitric oxide, has an inhibiting effect on swelling and exudation caused by acute inflammation and granulation hyperplasia caused by chronic inflammation in rat bodies and mice bodies and provides a theoretical and experimental basis for clinical medication of Monimopetalum Chinese for treating rheumatic arthritis.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method for extracting total triterpenoids from natural plant vine vine and its application in anti-inflammatory drugs. Background technique [0002] Monimopetalum Chinese Rehd. is a monotypic plant of the genus Monimopetalum in the family Celastraceae. It is a unique plant in China. The population is very rare, and it is listed as the first batch of second-class protected plants in the country. In 1925, Chinese botanist Qin Renchang collected fruit specimens for the first time in Pankeng, Zongli Village, Qimen County, Anhui Province, and published it in 1926. It was proposed to be named Qiteng at that time. According to the No. 3096 specimen collected by Qin Renchang, American plant taxonomist A. Rehder considers that there are large and persistent petals outside the fruit, which will never wither, which is quite rare in the plant kingdom, so i...

Claims

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Application Information

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IPC IPC(8): A61K36/37A61P29/00A61K127/00A61K135/00
CPCA61K36/37A61K2236/33A61K2236/331A61K2236/51A61K2236/53A61K2236/55
Inventor 印敏管福琴冯煦单宇王奇志陈雨赵友谊王鸣赵兴增
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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