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Acinetobacter strain for producing biosurfactant and application of acinetobacter strain

A surfactant and biological surface technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., to achieve good industrial application value, avoid secondary environmental pollution, and fast metabolism

Active Publication Date: 2016-04-20
SHANDONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And it has been submitted to the China General Microorganism Culture Collection Management Center for strain preservation

Method used

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  • Acinetobacter strain for producing biosurfactant and application of acinetobacter strain
  • Acinetobacter strain for producing biosurfactant and application of acinetobacter strain
  • Acinetobacter strain for producing biosurfactant and application of acinetobacter strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening of Acinetobacter sp. XH-2 strains producing biological surfactants.

[0021] Sampling from the soil contaminated by oil, the sample used in this embodiment is the soil sample polluted by crude oil at the Shengli Oil Field, Dongying, Shandong Province. Take 5g soil sample in 50mL enrichment medium (w / v, %: magnesium chloride 2, Dipotassium hydrogen phosphate 6, potassium dihydrogen phosphate 3.8, ferric chloride 0.5, ammonium chloride 10, additional preservative oil as the sole carbon source) for culture, take out 1mL of the culture medium and transfer it to the enrichment medium, transfer After inoculating for 3 times; dilute and spread on the blood plate, and streak and purify the strains with transparent circles on LB solid medium.

[0022] The purified single colony was inoculated in 5 mL of LB liquid medium, activated for 12 hours and then transferred to an unoptimized fermentation medium (w / v, %: glucose 1, peptone 1, potassium dihydrogen phosph...

Embodiment 2

[0025] Example 2 Fermentation process optimization of bacterial strain XH-2.

[0026] The fermentation medium and fermentation conditions of strain XH-2 were optimized based on surface tension and oil removal time.

[0027] Pick a single colony of strain XH-2 from the LB solid plate and inoculate it in 5 mL of LB liquid medium. After activating for 12 hours, transfer it to the fermentation medium.

[0028] Glucose, sucrose, lactose, whey powder, glycerin, olive oil, and fried dough stick waste oil were used as carbon sources, and peptone, sodium nitrate, ammonium sulfate, yeast extract (fermentation), and soybean meal were used as nitrogen sources. After 2 days of fermentation, Carry out the mensuration of surface tension and degreasing time, the results are shown in Table 1 and 2, mainly determine the most suitable carbon and nitrogen source according to degreasing time; Then carry out concentration optimization again, carbon source concentration is set to (w / v, %)0.1, 0.5 ,...

Embodiment 3

[0036] Example 3 Biosurfactant produced by fermentation of strain XH-2 was used to prepare biodegreaser.

[0037] Pick a single colony of the bacterial strain XH-2 on the LB solid plate and activate it with 5mL LB liquid medium for 12h, then transfer it to the fermentation medium optimized in Example 2 according to the inoculum size of 4%, and ferment and cultivate it on a shaker at 30°C and 160rpm for 2d After centrifuging the fermented liquid, take the supernatant and place it in a petri dish, place a 4cm long, 2cm wide, and 0.3cm high steel sheet in it, observe and record the time required for degreasing. see attached results image 3 , the picture A shows that the surface of the steel sheet is covered with oil film, the picture B shows that the oil film on the surface of the steel sheet has been removed, and the shortest degreasing time is 16 minutes, and the picture C shows that the steel sheet is left for a period of time after degreasing Finally, rust appears, indicati...

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Abstract

The invention provides an acinetobacter strain for producing a biosurfactant and an application of the acinetobacter strain. The acinetobacter strain is obtained from a petroleum foul land through enrichment culture, dilution coating, screening and purification and is authenticated as acinetobacter. The invention discloses an enrichment culture medium and a fermentation culture medium for screening the strain, and the properties of the biosurfactant produced from the strain are analyzed. A carbon source used in the culture media is whey powder which is leftover remained after the extraction of cheese and is low in price and cost; the strain is high in breeding speed and metabolism speed, the biosurfactant produced through the metabolism can act as a biological degreaser at the room temperature, and the strain is environmentally friendly, toxic-free, pollution-free and good in biodegradability; a high-temperature strong-alkali environment required by a traditional chemical method oil removing is avoided, the energy consumption and the operation danger coefficient are decreased, and the secondary environmental pollution is avoided; and the strain is stable in performance and has good industrial application value.

Description

technical field [0001] The invention relates to the application of Acinetobacter in the field of biotechnology, in particular to an Acinetobacter strain producing biosurfactant and its application. Background technique [0002] Biosurfactants are a class of microorganisms secreted during metabolism that contain hydrophilic groups (such as amino acids or polypeptides, disaccharides, oligosaccharides, or polysaccharides, etc.) and lipophilic groups (such as saturated or unsaturated fatty alcohols or fatty acids). etc.) amphiphilic compounds. In addition to the characteristics of chemical surfactants, biosurfactants also have the following characteristics: (1) high surface activity at the oil-water interface; (2) non-toxic, safe, easily biodegradable, and environmentally friendly; ( 3) It has strong oil emulsifying ability; (4) The reaction product can introduce new types of chemical groups, some of which are difficult to synthesize by chemical methods, and have various types ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C25D5/34C12R1/01
CPCC12N1/20C12P1/04C25D5/34C12N1/205C12R2001/01
Inventor 李慧娟孙云鹏丁瑞葛圣松崔志芳朱孔福李誉琦
Owner SHANDONG UNIV OF SCI & TECH
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