Method for increasing methane yield by preprocessing rice straw through rumen microorganisms
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A technology for rumen microorganisms and rice straw, which is applied in the directions of biosynthesis, waste fuel, fermentation, etc., can solve problems such as increasing methane production, and achieve the effects of increasing methane production, alleviating the pressure of discharging wastewater, and being convenient to operate.
Inactive Publication Date: 2016-04-20
HUNAN UNIV
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There is currently no method for pretreating ric...
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Embodiment 1
[0018] A method for improving methane production by pretreating rice straw with rumen microorganisms. The specific operation method is as follows:
[0019] (1) Filter the fresh rumen fluid with four layers of gauze, and store the filtrate in a thermos cup at 39°C;
[0020] (2) Mix the rice straw powder with the nutrient solution, the mixing ratio of the rice straw powder and the nutrient solution is 1.0g (dry weight): 50ml, insert the rumen fluid described in step (1), the volume of the rumen fluid and the nutrient solution The ratio is 0.5:1;
[0021] (3) Place the mixed solution in step (2) at 39° C. and culture it in a constant temperature oscillating water bath at 120 r / min for 120 h, adjust the initial pH to 7.0, and perform rumen microbial pretreatment;
[0022] (4) After the pretreatment, insert the anaerobic digestion inoculation sludge containing methanogens into the fermented liquid described in step (3), and the inoculation ratio of anaerobic inoculation sludge to...
Embodiment 2
[0025] A method for improving methane production by pretreating rice straw with rumen microorganisms. The specific operation method is as follows:
[0026] (1) Filter the fresh rumen fluid with four layers of gauze, and store the filtrate in a thermos cup at 39°C;
[0027] (2) Mix the rice straw powder with the nutrient solution, the mixing ratio of the rice straw powder and the nutrient solution is 5g (dry weight): 50ml, insert the rumen solution described in step (1), the volume ratio of the rumen solution and the nutrient solution 1:1;
[0028] (3) Place the mixed solution in step (2) at 39° C. and culture it in a constant temperature oscillating water bath at 120 r / min for 120 h, adjust the initial pH to 7.0, and perform rumen microbial pretreatment;
[0029] (4) After the pretreatment, insert the anaerobic digestion inoculation sludge containing methanogens into the fermented liquid described in step (3), and the inoculation ratio of the anaerobic inoculation sludge to ...
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Abstract
The invention discloses a method for increasing methane yield by preprocessing rice straw through rumen microorganisms, and belongs to the fields of biotechnologies and renewable energy. The method includes the steps of filtering fresh rumen fluid, storing filtrate at a certain temperature, mixing rice straw powder with a nutrient solution according to a certain proportion, inoculating rumen fluid of a certain proportion, placing the mixed fluid in a constant-temperature oscillation water bath kettle, conducting anaerobic cultivation at a certain temperature and a certain rotating speed, conducting rumen microorganism preprocessing, inoculating a certain proportion of anaerobic digestion inoculated sludge containing methanogens in the fermentation liquid after preprocessing ends, and conducting anaerobic digestion methanogenesis at a certain anaerobic digestion temperature and within the initial pH range. The rice straw biomass material is preprocessed through rumen microorganisms, hydrolytic acidification of the raw material is promoted, the high methane yield is obtained, and the reaction time is shortened; meanwhile, pressure for processing wastewater of slaughter houses on sewage treatment plants is relieved. Compared with the contrast group (not preprocessed), the methane yield is increased by 55.4-87.3%, the anaerobic digestion time is shortened by 3-10 days, the degradation rate of cellulose is increased by 20.3-33.6%, and the degradation rate of semi-cellulose is increased by 15.6-25.7%.
Description
technical field [0001] The invention belongs to the field of biotechnology renewable energy, and in particular relates to a method for improving methane production by using rumen microorganisms to pretreat rice straw. Background technique [0002] In recent years, on a global scale, with the increase of the population and the improvement of the quality of life, the demand for energy has risen sharply. More than 84% of the world's total energy comes from non-renewable fossil energy, such as coal, oil, and natural gas. When these fossil energy materials are burned, they inevitably emit greenhouse gases into the atmosphere and pollute the environment. Biogas (methane), produced by anaerobic digestion of renewable resources (lignocellulose), is considered an ideal energy source that can replace fossil fuels. Anaerobic digestion can generally be divided into four stages: hydrolysis, acidification, acetic acidification, and methanation. The microorganisms involved in these four...
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