Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method

A technology of photothermal therapy and photoacoustic imaging, which is applied in the field of medicine, can solve the problems of low yield, complicated preparation process, and low biocompatibility, and achieve the effects of low energy consumption, simple preparation method, and strong near-infrared absorption

Inactive Publication Date: 2018-10-19
TIANJIN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported polyaniline nanoparticles used for photothermal therapy use surfactants such as poloxamer, polyethylene glycol, etc. as stabilizers, which have low biocompatibility, and the preparation process is complicated and the yield is low.
In addition, there are few reports about polyaniline nanomaterials as contrast agents for photoacoustic imaging.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method
  • Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method
  • Phospholipid-polyaniline nanoparticles for photoacoustic imaging and/or photothermal therapy and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of intrinsic state polyaniline: (adopt oxidation polymerization method, this method is reported in the literature)

[0038] Add 0.2mol aniline monomer and 0.3mol concentrated hydrochloric acid to 300mL water to obtain solution a; add 0.1mol ammonium persulfate and 0.2mol concentrated hydrochloric acid to 200ml water to obtain solution b; under stirring, add solution b dropwise to the solution In a, stir at 4°C for 6 hours to obtain a polymer salt solution, which is filtered; disperse all the obtained green precipitates into 500 ml of 1M aqueous sodium hydroxide solution for dedoping reaction, filter, and remove all the obtained purple The precipitate was dispersed into 500 ml of acetone, filtered, and dried under vacuum for 20 hours to obtain purple intrinsic polyaniline powder, which is called EB PANI for short. Gel chromatographic column detection molecular weight 5000.

Embodiment 2

[0040] A method for preparing phospholipid-polyaniline nanoparticles for photoacoustic imaging and / or photothermal therapy, comprising the steps of:

[0041] (1) the intrinsic state polyaniline (the intrinsic state polyaniline is called for short EB PANI) that molecular weight is 5000 is prepared by oxidative polymerization method (see embodiment 1);

[0042] (2) Synthesis of polyaniline nanoparticles wrapped in phospholipids: 5 mg of the intrinsic polyaniline obtained in step (1) and 50 mg of sodium dodecylbenzenesulfonate were dissolved in 8 mL of chloroform; stirred at room temperature for 18 hours to obtain a green Conductive polyaniline solution (conductive polyaniline referred to as ES PANI), 8mL conductive polyaniline solution, 20mL chloroform, 35.2mgDPPC, 5mgDSPE-PEG2000 and 1.4mgFA-DSPE-PEG2000 were added to the container, ultrasonically dispersed for 4 minutes; Evaporate under reduced pressure at 50°C and 120rpm for 25 minutes to remove chloroform, and stand in vacuu...

Embodiment 3

[0044] A method for preparing phospholipid-polyaniline nanoparticles for photoacoustic imaging and / or photothermal therapy, comprising the steps of:

[0045] (1) the intrinsic state polyaniline that molecular weight is 5000 is prepared by oxidative polymerization method (see embodiment 1);

[0046](2) Synthesis of polyaniline nanoparticles wrapped in phospholipids: 5 mg of the intrinsic polyaniline obtained in step (1) and 35 mg of sodium dodecylbenzenesulfonate were dissolved in 5 mL of chloroform; stirred at room temperature for 12 hours to obtain a green Conductive polyaniline solution (conductive polyaniline referred to as ES PANI), 5mL conductive polyaniline solution, 20mL chloroform, 35.2mgDPPC, 4mgDSPE-PEG2000 and 1.2mgFA-DSPE-PEG2000 were added to the container, ultrasonically dispersed for 2 minutes; Evaporate under reduced pressure at 40°C and 100rpm for 20 minutes to remove chloroform, and stand in vacuum for 6 hours to remove residual chloroform, add 5mL of water, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses phospholipid-polyaniline nanoparticles for photoacoustic imaging and / or photothermal therapy and a preparation method. The preparation method comprises the steps that 1, eigenstate polyaniline is prepared; 2, phospholipid-coated polyaniline nanoparticles are synthesized, wherein eigenstate polyaniline and sodium dodecyl benzene sulfonate are dissolved into chloroform, stirring is performed to obtain a green conductive polyaniline solution, the conductive polyaniline solution, chloroform, DPPC, DSPE-PEG2000 and FA-DSPE-PEG2000 are added into a container, ultrasonic dispersion is performed, chloroform is removed through evaporation, water is added, ultrasonic dispersion and centrifugation are performed, supernate is a clear and bright green solution, and then freeze drying is performed. The preparation method has the advantages of being simple, high in reaction controllability, low in energy dissipation and capable of easily achieving scalization and easily obtaining the raw materials; the prepared nanoparticles show the good water solubility and biocompatibility, the high near-infrared absorption performance and the good light and thermal stability and can be simultaneously used for photothermal therapy, photoacoustic imaging and infrared thermal imaging.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a polyaniline nanoparticle used for photoacoustic imaging and photothermal therapy and a preparation method. Background technique [0002] Photothermal therapy is a minimally invasive tumor treatment technology developed in recent years. It mainly kills tumor cells by directly irradiating light energy to the tumor site to increase its local temperature, which greatly reduces systemic toxicity. Therefore, photothermal therapy It is regarded as one of the techniques for treating tumors with great potential to replace surgery. To improve the efficiency and tumor selectivity of laser-induced photothermal therapy, photothermal therapeutic agents with light-absorbing properties are usually introduced into tumor sites. Since water and protein in biological tissues absorb near-infrared light relatively weakly, so near-infrared light has the best penetrability to tissue, an ideal photothermal thera...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K49/22A61K47/24
CPCA61K41/0052A61K47/24A61K49/221A61K49/225
Inventor 王金平过芳于梦谭丰苹李楠
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com